@article{messinger_detection_1995,
	title = {Detection of one slowly exchanging substrate water molecule in the {S3} state of photosystem {II}.},
	volume = {92},
	url = {https://www.pnas.org/doi/10.1073/pnas.92.8.3209},
	doi = {10.1073/pnas.92.8.3209},
	abstract = {The exchangeability of the substrate water molecules at the catalytic site of water oxidation in photosystem II has been probed by isotope-exchange measurements using mass spectrometric detection of flash-induced oxygen evolution. A stirred sample chamber was constructed to reduce the lag time between injection of H2(18)O and the detecting flash by a factor of more than 1000 compared to the original experiments by R. Radmer and O. Ollinger [(1986) FEBS Lett. 195, 285-289]. Our data show that there is a slow (t1/2 approximately 500 ms, 10 degrees C) and a fast (t1/2 {\textless}25 ms, 10 degrees C) exchanging substrate water molecule in the S3 state of photosystem II. The slow exchange is coupled with an activation energy of about 75 kJ/mol and is discussed in terms of a terminal manganese oxo ligand, while the faster exchanging substrate molecule may represent a water molecule not directly bound to the manganese center.},
	number = {8},
	urldate = {2024-11-28},
	journal = {Proceedings of the National Academy of Sciences},
	author = {Messinger, J and Badger, M and Wydrzynski, T},
	month = apr,
	year = {1995},
	note = {Publisher: Proceedings of the National Academy of Sciences},
	pages = {3209--3213},
}

{"_id":"9aKQHkhCiyroE67Ki","bibbaseid":"messinger-badger-wydrzynski-detectionofoneslowlyexchangingsubstratewatermoleculeinthes3stateofphotosystemii-1995","author_short":["Messinger, J","Badger, M","Wydrzynski, T"],"bibdata":{"bibtype":"article","type":"article","title":"Detection of one slowly exchanging substrate water molecule in the S3 state of photosystem II.","volume":"92","url":"https://www.pnas.org/doi/10.1073/pnas.92.8.3209","doi":"10.1073/pnas.92.8.3209","abstract":"The exchangeability of the substrate water molecules at the catalytic site of water oxidation in photosystem II has been probed by isotope-exchange measurements using mass spectrometric detection of flash-induced oxygen evolution. A stirred sample chamber was constructed to reduce the lag time between injection of H2(18)O and the detecting flash by a factor of more than 1000 compared to the original experiments by R. Radmer and O. Ollinger [(1986) FEBS Lett. 195, 285-289]. Our data show that there is a slow (t1/2 approximately 500 ms, 10 degrees C) and a fast (t1/2 \\textless25 ms, 10 degrees C) exchanging substrate water molecule in the S3 state of photosystem II. The slow exchange is coupled with an activation energy of about 75 kJ/mol and is discussed in terms of a terminal manganese oxo ligand, while the faster exchanging substrate molecule may represent a water molecule not directly bound to the manganese center.","number":"8","urldate":"2024-11-28","journal":"Proceedings of the National Academy of Sciences","author":[{"propositions":[],"lastnames":["Messinger"],"firstnames":["J"],"suffixes":[]},{"propositions":[],"lastnames":["Badger"],"firstnames":["M"],"suffixes":[]},{"propositions":[],"lastnames":["Wydrzynski"],"firstnames":["T"],"suffixes":[]}],"month":"April","year":"1995","note":"Publisher: Proceedings of the National Academy of Sciences","pages":"3209–3213","bibtex":"@article{messinger_detection_1995,\n\ttitle = {Detection of one slowly exchanging substrate water molecule in the {S3} state of photosystem {II}.},\n\tvolume = {92},\n\turl = {https://www.pnas.org/doi/10.1073/pnas.92.8.3209},\n\tdoi = {10.1073/pnas.92.8.3209},\n\tabstract = {The exchangeability of the substrate water molecules at the catalytic site of water oxidation in photosystem II has been probed by isotope-exchange measurements using mass spectrometric detection of flash-induced oxygen evolution. A stirred sample chamber was constructed to reduce the lag time between injection of H2(18)O and the detecting flash by a factor of more than 1000 compared to the original experiments by R. Radmer and O. Ollinger [(1986) FEBS Lett. 195, 285-289]. Our data show that there is a slow (t1/2 approximately 500 ms, 10 degrees C) and a fast (t1/2 {\\textless}25 ms, 10 degrees C) exchanging substrate water molecule in the S3 state of photosystem II. The slow exchange is coupled with an activation energy of about 75 kJ/mol and is discussed in terms of a terminal manganese oxo ligand, while the faster exchanging substrate molecule may represent a water molecule not directly bound to the manganese center.},\n\tnumber = {8},\n\turldate = {2024-11-28},\n\tjournal = {Proceedings of the National Academy of Sciences},\n\tauthor = {Messinger, J and Badger, M and Wydrzynski, T},\n\tmonth = apr,\n\tyear = {1995},\n\tnote = {Publisher: Proceedings of the National Academy of Sciences},\n\tpages = {3209--3213},\n}\n\n\n\n","author_short":["Messinger, J","Badger, M","Wydrzynski, T"],"key":"messinger_detection_1995","id":"messinger_detection_1995","bibbaseid":"messinger-badger-wydrzynski-detectionofoneslowlyexchangingsubstratewatermoleculeinthes3stateofphotosystemii-1995","role":"author","urls":{"Paper":"https://www.pnas.org/doi/10.1073/pnas.92.8.3209"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["9cGcv2t8pRzC92kzs"],"keywords":[],"search_terms":["detection","one","slowly","exchanging","substrate","water","molecule","state","photosystem","messinger","badger","wydrzynski"],"title":"Detection of one slowly exchanging substrate water molecule in the S3 state of photosystem II.","year":1995}