@article{
 title = {Transgenic Broccoli Expressing a Bacillus-Thuringiensis Insecticidal Crystal Protein - Implications for Pest Resistance Management Strategies},
 type = {article},
 year = {1995},
 pages = {309-317},
 volume = {1},
 websites = {<Go to ISI>://A1995TK04600001},
 id = {9afcf2ca-95c1-3592-84e9-8949df9a7e67},
 created = {2012-01-05T13:07:25.000Z},
 file_attached = {false},
 profile_id = {1a467167-0a41-3583-a6a3-034c31031332},
 group_id = {0e532975-1a47-38a4-ace8-4fe5968bcd72},
 last_modified = {2012-01-05T13:14:39.000Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 source_type = {Journal Article},
 abstract = {We used Agrobacterium tumefaciens to transform flowering stalk explants of five genotypes of broccoli with a construct containing the neomycin phosphotransferase gene and a Bacillus thuringiensis (Bt) gene [CryIA(c) type] optimized for plant expression. Overall transformation efficiency was 6.4%; 181 kanamycin-resistant plants were recovered. Of the 162 kanamycin-resistant plants tested, 112 (69%) caused 100% morality of 1st-instar larvae of a Bt-susceptible diamondback moth strain. Southern blots of some resistant transformants confirmed presence of the Bt gene. Selected plants that gave 100% mortality of susceptible larvae allowed survival of a strain of diamondback moth that had evolved resistance to Bt in the field. F-1 hybrids between resistant and susceptible insects did not survive. Analysis of progeny from 26 resistant transgenic lines showed 16 that gave segregation ratios consistent with a single T-DNA integration. Southern analysis was used to verify those plants possessing a single T-DNA integration. Because these transgenic plants kill susceptible larvae and F, larvae, but serve as a suitable host for resistant ones, they provide an excellent model for tests of Bt resistance management strategies.},
 bibtype = {article},
 author = {Metz, T D and Roush, R T and Tang, J D and Shelton, A M and Earle, E D},
 journal = {Molecular Breeding},
 number = {4}
}

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