Sugar-activated ion transport in canine lingual epithelium. Implications for sugar taste transduction. Mierson, S, DeSimone, S K, Heck, G L, & DeSimone, J A J Gen Physiol, 92(1):87–111, 1988. Place: UNITED STATES ISBN: 0022-1295abstract bibtex There is good evidence indicating that ion-transport pathways in the apical regions of lingual epithelial cells, including taste bud cells, may play a role in salt taste reception. In this article, we present evidence that, in the case of the dog, there also exists a sugar-activated ion-transport pathway that is linked to sugar taste transduction. Evidence was drawn from two parallel lines of experiments: (a) ion-transport studies on the isolated canine lingual epithelium, and (b) recordings from the canine chorda tympani. The results in vitro showed that both mono- and disaccharides in the mucosal bath stimulate a dose-dependent increase in the short-circuit current over the concentration range coincident with mammalian sugar taste responses. Transepithelial current evoked by glucose, fructose, or sucrose in either 30 mM NaCl or in Krebs-Henseleit buffer (K-H) was partially blocked by amiloride. Among current carriers activated by saccharides, the current response was greater with Na than with K. Ion flux measurements in K-H during stimulation with 3-O-methylglucose showed that the sugar-evoked current was due to an increase in the Na influx. Ouabain or amiloride reduced the sugar-evoked Na influx without effect on sugar transport as measured with tritiated 3-O-methylglucose. Amiloride inhibited the canine chorda tympani response to 0.5 M NaCl by 70-80% and the response to 0.5 M KCl by approximately 40%. This agreed with the percent inhibition by amiloride of the short-circuit current supported in vitro by NaCl and KCl. Amiloride also partially inhibited the chorda tympani responses to sucrose and to fructose. The results indicate that in the dog: (a) the ion transporter subserving Na taste also subserves part of the response to K, and (b) a sugar-activated, Na-preferring ion-transport system is one mechanism mediating sugar taste transduction. Results in the literature indicate a similar sweet taste mechanism for humans.
@article{mierson_sugar-activated_1988,
title = {Sugar-activated ion transport in canine lingual epithelium. {Implications} for sugar taste transduction.},
volume = {92},
abstract = {There is good evidence indicating that ion-transport pathways in the apical regions of lingual epithelial cells, including taste bud cells, may play a role in salt taste reception. In this article, we present evidence that, in the case of the dog, there also exists a sugar-activated ion-transport pathway that is linked to sugar taste transduction. Evidence was drawn from two parallel lines of experiments: (a) ion-transport studies on the isolated canine lingual epithelium, and (b) recordings from the canine chorda tympani. The results in vitro showed that both mono- and disaccharides in the mucosal bath stimulate a dose-dependent increase in the short-circuit current over the concentration range coincident with mammalian sugar taste responses. Transepithelial current evoked by glucose, fructose, or sucrose in either 30 mM NaCl or in Krebs-Henseleit buffer (K-H) was partially blocked by amiloride. Among current carriers activated by saccharides, the current response was greater with Na than with K. Ion flux measurements in K-H during stimulation with 3-O-methylglucose showed that the sugar-evoked current was due to an increase in the Na influx. Ouabain or amiloride reduced the sugar-evoked Na influx without effect on sugar transport as measured with tritiated 3-O-methylglucose. Amiloride inhibited the canine chorda tympani response to 0.5 M NaCl by 70-80\% and the response to 0.5 M KCl by approximately 40\%. This agreed with the percent inhibition by amiloride of the short-circuit current supported in vitro by NaCl and KCl. Amiloride also partially inhibited the chorda tympani responses to sucrose and to fructose. The results indicate that in the dog: (a) the ion transporter subserving Na taste also subserves part of the response to K, and (b) a sugar-activated, Na-preferring ion-transport system is one mechanism mediating sugar taste transduction. Results in the literature indicate a similar sweet taste mechanism for humans.},
language = {eng},
number = {1},
journal = {J Gen Physiol},
author = {Mierson, S and DeSimone, S K and Heck, G L and DeSimone, J A},
year = {1988},
pmid = {3171536},
note = {Place: UNITED STATES
ISBN: 0022-1295},
keywords = {Amiloride, Animals, Biological Transport, Carbohydrates, Chorda Tympani Nerve, Dogs, Electric Conductivity, Epithelium, Ions, Phlorhizin, Potassium Chloride, Sodium Chloride, Taste, Tongue, comparative study, research support, non-u.s. gov't, research support, u.s. gov't, non-p.h.s.},
pages = {87--111},
}
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The results in vitro showed that both mono- and disaccharides in the mucosal bath stimulate a dose-dependent increase in the short-circuit current over the concentration range coincident with mammalian sugar taste responses. Transepithelial current evoked by glucose, fructose, or sucrose in either 30 mM NaCl or in Krebs-Henseleit buffer (K-H) was partially blocked by amiloride. Among current carriers activated by saccharides, the current response was greater with Na than with K. Ion flux measurements in K-H during stimulation with 3-O-methylglucose showed that the sugar-evoked current was due to an increase in the Na influx. Ouabain or amiloride reduced the sugar-evoked Na influx without effect on sugar transport as measured with tritiated 3-O-methylglucose. Amiloride inhibited the canine chorda tympani response to 0.5 M NaCl by 70-80% and the response to 0.5 M KCl by approximately 40%. This agreed with the percent inhibition by amiloride of the short-circuit current supported in vitro by NaCl and KCl. Amiloride also partially inhibited the chorda tympani responses to sucrose and to fructose. The results indicate that in the dog: (a) the ion transporter subserving Na taste also subserves part of the response to K, and (b) a sugar-activated, Na-preferring ion-transport system is one mechanism mediating sugar taste transduction. Results in the literature indicate a similar sweet taste mechanism for humans.","language":"eng","number":"1","journal":"J Gen Physiol","author":[{"propositions":[],"lastnames":["Mierson"],"firstnames":["S"],"suffixes":[]},{"propositions":[],"lastnames":["DeSimone"],"firstnames":["S","K"],"suffixes":[]},{"propositions":[],"lastnames":["Heck"],"firstnames":["G","L"],"suffixes":[]},{"propositions":[],"lastnames":["DeSimone"],"firstnames":["J","A"],"suffixes":[]}],"year":"1988","pmid":"3171536","note":"Place: UNITED STATES ISBN: 0022-1295","keywords":"Amiloride, Animals, Biological Transport, Carbohydrates, Chorda Tympani Nerve, Dogs, Electric Conductivity, Epithelium, Ions, Phlorhizin, Potassium Chloride, Sodium Chloride, Taste, Tongue, comparative study, research support, non-u.s. gov't, research support, u.s. gov't, non-p.h.s.","pages":"87–111","bibtex":"@article{mierson_sugar-activated_1988,\n\ttitle = {Sugar-activated ion transport in canine lingual epithelium. {Implications} for sugar taste transduction.},\n\tvolume = {92},\n\tabstract = {There is good evidence indicating that ion-transport pathways in the apical regions of lingual epithelial cells, including taste bud cells, may play a role in salt taste reception. In this article, we present evidence that, in the case of the dog, there also exists a sugar-activated ion-transport pathway that is linked to sugar taste transduction. Evidence was drawn from two parallel lines of experiments: (a) ion-transport studies on the isolated canine lingual epithelium, and (b) recordings from the canine chorda tympani. The results in vitro showed that both mono- and disaccharides in the mucosal bath stimulate a dose-dependent increase in the short-circuit current over the concentration range coincident with mammalian sugar taste responses. Transepithelial current evoked by glucose, fructose, or sucrose in either 30 mM NaCl or in Krebs-Henseleit buffer (K-H) was partially blocked by amiloride. Among current carriers activated by saccharides, the current response was greater with Na than with K. Ion flux measurements in K-H during stimulation with 3-O-methylglucose showed that the sugar-evoked current was due to an increase in the Na influx. Ouabain or amiloride reduced the sugar-evoked Na influx without effect on sugar transport as measured with tritiated 3-O-methylglucose. Amiloride inhibited the canine chorda tympani response to 0.5 M NaCl by 70-80\\% and the response to 0.5 M KCl by approximately 40\\%. This agreed with the percent inhibition by amiloride of the short-circuit current supported in vitro by NaCl and KCl. Amiloride also partially inhibited the chorda tympani responses to sucrose and to fructose. The results indicate that in the dog: (a) the ion transporter subserving Na taste also subserves part of the response to K, and (b) a sugar-activated, Na-preferring ion-transport system is one mechanism mediating sugar taste transduction. Results in the literature indicate a similar sweet taste mechanism for humans.},\n\tlanguage = {eng},\n\tnumber = {1},\n\tjournal = {J Gen Physiol},\n\tauthor = {Mierson, S and DeSimone, S K and Heck, G L and DeSimone, J A},\n\tyear = {1988},\n\tpmid = {3171536},\n\tnote = {Place: UNITED STATES\nISBN: 0022-1295},\n\tkeywords = {Amiloride, Animals, Biological Transport, Carbohydrates, Chorda Tympani Nerve, Dogs, Electric Conductivity, Epithelium, Ions, Phlorhizin, Potassium Chloride, Sodium Chloride, Taste, Tongue, comparative study, research support, non-u.s. gov't, research support, u.s. gov't, non-p.h.s.},\n\tpages = {87--111},\n}\n\n","author_short":["Mierson, S","DeSimone, S K","Heck, G L","DeSimone, J A"],"key":"mierson_sugar-activated_1988","id":"mierson_sugar-activated_1988","bibbaseid":"mierson-desimone-heck-desimone-sugaractivatediontransportincaninelingualepitheliumimplicationsforsugartastetransduction-1988","role":"author","urls":{},"keyword":["Amiloride","Animals","Biological Transport","Carbohydrates","Chorda Tympani Nerve","Dogs","Electric Conductivity","Epithelium","Ions","Phlorhizin","Potassium Chloride","Sodium Chloride","Taste","Tongue","comparative study","research support","non-u.s. gov't","research support","u.s. gov't","non-p.h.s."],"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/zotero/michaeltarr","dataSources":["5qXSH7BrePnXHtcrf"],"keywords":["amiloride","animals","biological transport","carbohydrates","chorda tympani nerve","dogs","electric conductivity","epithelium","ions","phlorhizin","potassium chloride","sodium chloride","taste","tongue","comparative study","research support","non-u.s. gov't","research support","u.s. gov't","non-p.h.s."],"search_terms":["sugar","activated","ion","transport","canine","lingual","epithelium","implications","sugar","taste","transduction","mierson","desimone","heck","desimone"],"title":"Sugar-activated ion transport in canine lingual epithelium. Implications for sugar taste transduction.","year":1988}