@article{
 title = {Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node},
 type = {article},
 year = {2002},
 identifiers = {[object Object]},
 pages = {1869-1873},
 volume = {296},
 websites = {http://www.sciencemag.org/cgi/doi/10.1126/science.1070051},
 id = {a35cb912-0c3c-384c-b11c-54368f9c122f},
 created = {2018-03-05T02:58:05.413Z},
 file_attached = {true},
 profile_id = {fc9ef84f-888b-33fd-b1d3-fbf022f2f0c8},
 group_id = {21bf6821-cbba-3be9-9d50-072f76d57584},
 last_modified = {2018-03-05T02:58:05.519Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 citation_key = {Miller2002},
 private_publication = {false},
 abstract = {Lymphocyte motility is vital for trafficking within lymphoid organs and for initiating contact with antigen-presenting cells. Visualization of these processes has previously been limited to in vitro systems. We describe the use of two-photon laser microscopy to image the dynamic behavior of individual living lymphocytes deep within intact lymph nodes. In their native environment, T cells achieved peak velocities of more than 25 micrometers per minute, displaying a motility coefficient that is five to six times that of B cells. Antigenic challenge changed T cell trajectories from random walks to "swarms" and stable clusters. Real-time two-photon imaging reveals lymphocyte behaviors that are fundamental to the initiation of the immune response.},
 bibtype = {article},
 author = {Miller, M. J.},
 journal = {Science},
 number = {5574}
}

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