Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts. Montero, L., Sánchez-Camargo, A. P., García-Cañas, V., Tanniou, A., Stiger-Pouvreau, V., Russo, M., Rastrelli, L., Cifuentes, A., Herrero, M., & Ibáñez, E. Journal of Chromatography A, 1428:115--125, 2016.
Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts [link]Paper  doi  abstract   bibtex   
In the present work, the phlorotannin composition of different Sargassum muticum samples collected at different locations along the North Atlantic coasts as well as the bioactivities related to these components were investigated. After pressurized liquid extraction, the samples collected at the extreme locations of a latitudinal gradient from Portugal and Norway, were found to be the richest on total phenols and, particularly, on phlorotannins, containing up to 148.97 and 5.12 mg phloroglucinol equivalents g−1, respectively. The extracts obtained from these locations were further purified and chemically characterized using a modified HILIC × RP-DAD-MS/MS method. The application of this methodology allowed the tentative identification of a great variability of phlorotannins with different degrees of polymerization (from 3 to 11) and structures, determined for the first time in S. muticum. The most-abundant phlorotannins on these samples were fuhalols, hydroxyfuhalols and phlorethols, showing also particularities and important differences depending on the geographical location. Afterwards, the antiproliferative activity of these extracts against HT-29 adenocarcinoma colon cancer cells was studied. Results revealed that the richest S. muticum samples in terms of total phlorotannins, i.e., those from Norway, presented the highest activity, showing a good cytotoxic potential at concentrations in the medium micromolar range.
@article{montero_anti-proliferative_2016,
	title = {Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga {Sargassum} muticum collected on {North}-{Atlantic} coasts},
	volume = {1428},
	issn = {0021-9673},
	url = {http://www.sciencedirect.com/science/article/pii/S0021967315010213},
	doi = {10.1016/j.chroma.2015.07.053},
	abstract = {In the present work, the phlorotannin composition of different Sargassum muticum samples collected at different locations along the North Atlantic coasts as well as the bioactivities related to these components were investigated. After pressurized liquid extraction, the samples collected at the extreme locations of a latitudinal gradient from Portugal and Norway, were found to be the richest on total phenols and, particularly, on phlorotannins, containing up to 148.97 and 5.12 mg phloroglucinol equivalents g−1, respectively. The extracts obtained from these locations were further purified and chemically characterized using a modified HILIC × RP-DAD-MS/MS method. The application of this methodology allowed the tentative identification of a great variability of phlorotannins with different degrees of polymerization (from 3 to 11) and structures, determined for the first time in S. muticum. The most-abundant phlorotannins on these samples were fuhalols, hydroxyfuhalols and phlorethols, showing also particularities and important differences depending on the geographical location. Afterwards, the antiproliferative activity of these extracts against HT-29 adenocarcinoma colon cancer cells was studied. Results revealed that the richest S. muticum samples in terms of total phlorotannins, i.e., those from Norway, presented the highest activity, showing a good cytotoxic potential at concentrations in the medium micromolar range.},
	urldate = {2015-07-24TZ},
	journal = {Journal of Chromatography A},
	author = {Montero, Lidia and Sánchez-Camargo, Andrea P. and García-Cañas, Virginia and Tanniou, Anaëlle and Stiger-Pouvreau, Valérie and Russo, Mariateresa and Rastrelli, Luca and Cifuentes, Alejandro and Herrero, Miguel and Ibáñez, Elena},
	year = {2016},
	keywords = {ACL, Anti-proliferative activity, Brown algae, Comprehensive LC, E2, Phenolic compounds, Phlorotannins, Sargassum muticum},
	pages = {115--125}
}
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