@article{
 title = {A high-throughput screen for inhibitors of the prolyl isomerase, Pin1, identifies a seaweed polyphenol that reduces adipose cell differentiation},
 type = {article},
 year = {2014},
 identifiers = {[object Object]},
 keywords = {Adipose cell,High-throughput screen,Obesity,Prolyl isomerase,Seaweed polyphenol},
 volume = {78},
 id = {fdb49483-05f5-361d-8e58-ddba93b42d15},
 created = {2018-02-28T22:42:48.647Z},
 file_attached = {false},
 profile_id = {a39b8b7f-45f4-3d11-94d2-dbc38be2487d},
 last_modified = {2018-02-28T22:42:48.647Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {false},
 hidden = {false},
 private_publication = {true},
 abstract = {© 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry. The peptidyl prolyl cis/trans isomerase Pin1 enhances the uptake of triglycerides and the differentiation of fibroblasts into adipose cells in response to insulin stimulation. Pin1 downregulation could be a potential approach to prevent and treat obesity-related disorders. In order to identify an inhibitor of Pin1 that exhibited minimal cytotoxicity, we established a high-throughput screen for Pin1 inhibitors and used this method to identify an inhibitor from 1,056 crude fractions of two natural product libraries. The candidate, a phlorotannin called 974-B, was isolated from the seaweed, Ecklonia kurome. 974-B inhibited the differentiation of mouse embryonic fibroblasts and 3T3-L1 cells into adipose cells without inducing cytotoxicity. We discovered the Pin1 inhibitor, 974-B, from the seaweed, E. kurome, and showed that it blocks the differentiation of fibroblasts into adipose cells, suggesting that 974-B could be a lead drug candidate for obesity-related disorders.},
 bibtype = {article},
 author = {Mori, T. and Hidaka, M. and Ikuji, H. and Yoshizawa, I. and Toyohara, H. and Okuda, T. and Uchida, C. and Asano, T. and Yotsu-Yamashita, M. and Uchida, T.},
 journal = {Bioscience, Biotechnology and Biochemistry},
 number = {5}
}

{"_id":"KcRPkoZ9qyWuoe2HG","bibbaseid":"mori-hidaka-ikuji-yoshizawa-toyohara-okuda-uchida-asano-etal-ahighthroughputscreenforinhibitorsoftheprolylisomerasepin1identifiesaseaweedpolyphenolthatreducesadiposecelldifferentiation-2014","authorIDs":["5da02595c76cddda01000033"],"author_short":["Mori, T.","Hidaka, M.","Ikuji, H.","Yoshizawa, I.","Toyohara, H.","Okuda, T.","Uchida, C.","Asano, T.","Yotsu-Yamashita, M.","Uchida, T."],"bibdata":{"title":"A high-throughput screen for inhibitors of the prolyl isomerase, Pin1, identifies a seaweed polyphenol that reduces adipose cell differentiation","type":"article","year":"2014","identifiers":"[object Object]","keywords":"Adipose cell,High-throughput screen,Obesity,Prolyl isomerase,Seaweed polyphenol","volume":"78","id":"fdb49483-05f5-361d-8e58-ddba93b42d15","created":"2018-02-28T22:42:48.647Z","file_attached":false,"profile_id":"a39b8b7f-45f4-3d11-94d2-dbc38be2487d","last_modified":"2018-02-28T22:42:48.647Z","read":false,"starred":false,"authored":"true","confirmed":false,"hidden":false,"private_publication":"true","abstract":"© 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry. The peptidyl prolyl cis/trans isomerase Pin1 enhances the uptake of triglycerides and the differentiation of fibroblasts into adipose cells in response to insulin stimulation. Pin1 downregulation could be a potential approach to prevent and treat obesity-related disorders. In order to identify an inhibitor of Pin1 that exhibited minimal cytotoxicity, we established a high-throughput screen for Pin1 inhibitors and used this method to identify an inhibitor from 1,056 crude fractions of two natural product libraries. The candidate, a phlorotannin called 974-B, was isolated from the seaweed, Ecklonia kurome. 974-B inhibited the differentiation of mouse embryonic fibroblasts and 3T3-L1 cells into adipose cells without inducing cytotoxicity. We discovered the Pin1 inhibitor, 974-B, from the seaweed, E. kurome, and showed that it blocks the differentiation of fibroblasts into adipose cells, suggesting that 974-B could be a lead drug candidate for obesity-related disorders.","bibtype":"article","author":"Mori, T. and Hidaka, M. and Ikuji, H. and Yoshizawa, I. and Toyohara, H. and Okuda, T. and Uchida, C. and Asano, T. and Yotsu-Yamashita, M. and Uchida, T.","journal":"Bioscience, Biotechnology and Biochemistry","number":"5","bibtex":"@article{\n title = {A high-throughput screen for inhibitors of the prolyl isomerase, Pin1, identifies a seaweed polyphenol that reduces adipose cell differentiation},\n type = {article},\n year = {2014},\n identifiers = {[object Object]},\n keywords = {Adipose cell,High-throughput screen,Obesity,Prolyl isomerase,Seaweed polyphenol},\n volume = {78},\n id = {fdb49483-05f5-361d-8e58-ddba93b42d15},\n created = {2018-02-28T22:42:48.647Z},\n file_attached = {false},\n profile_id = {a39b8b7f-45f4-3d11-94d2-dbc38be2487d},\n last_modified = {2018-02-28T22:42:48.647Z},\n read = {false},\n starred = {false},\n authored = {true},\n confirmed = {false},\n hidden = {false},\n private_publication = {true},\n abstract = {© 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry. The peptidyl prolyl cis/trans isomerase Pin1 enhances the uptake of triglycerides and the differentiation of fibroblasts into adipose cells in response to insulin stimulation. Pin1 downregulation could be a potential approach to prevent and treat obesity-related disorders. In order to identify an inhibitor of Pin1 that exhibited minimal cytotoxicity, we established a high-throughput screen for Pin1 inhibitors and used this method to identify an inhibitor from 1,056 crude fractions of two natural product libraries. The candidate, a phlorotannin called 974-B, was isolated from the seaweed, Ecklonia kurome. 974-B inhibited the differentiation of mouse embryonic fibroblasts and 3T3-L1 cells into adipose cells without inducing cytotoxicity. We discovered the Pin1 inhibitor, 974-B, from the seaweed, E. kurome, and showed that it blocks the differentiation of fibroblasts into adipose cells, suggesting that 974-B could be a lead drug candidate for obesity-related disorders.},\n bibtype = {article},\n author = {Mori, T. and Hidaka, M. and Ikuji, H. and Yoshizawa, I. and Toyohara, H. and Okuda, T. and Uchida, C. and Asano, T. and Yotsu-Yamashita, M. and Uchida, T.},\n journal = {Bioscience, Biotechnology and Biochemistry},\n number = {5}\n}","author_short":["Mori, T.","Hidaka, M.","Ikuji, H.","Yoshizawa, I.","Toyohara, H.","Okuda, T.","Uchida, C.","Asano, T.","Yotsu-Yamashita, M.","Uchida, T."],"bibbaseid":"mori-hidaka-ikuji-yoshizawa-toyohara-okuda-uchida-asano-etal-ahighthroughputscreenforinhibitorsoftheprolylisomerasepin1identifiesaseaweedpolyphenolthatreducesadiposecelldifferentiation-2014","role":"author","urls":{},"keyword":["Adipose cell","High-throughput screen","Obesity","Prolyl isomerase","Seaweed polyphenol"],"downloads":0,"html":""},"bibtype":"article","creationDate":"2019-10-11T06:47:49.309Z","downloads":0,"keywords":["adipose cell","high-throughput screen","obesity","prolyl isomerase","seaweed polyphenol"],"search_terms":["high","throughput","screen","inhibitors","prolyl","isomerase","pin1","identifies","seaweed","polyphenol","reduces","adipose","cell","differentiation","mori","hidaka","ikuji","yoshizawa","toyohara","okuda","uchida","asano","yotsu-yamashita","uchida"],"title":"A high-throughput screen for inhibitors of the prolyl isomerase, Pin1, identifies a seaweed polyphenol that reduces adipose cell differentiation","year":2014}