@article{Moriyama2011,
	title = {{DNA} assembly and re-assembly activated by cationic comb-type copolymer},
	volume = {32},
	issn = {01429612},
	url = {http://dx.doi.org/10.1016/j.biomaterials.2010.11.064 http://www.ncbi.nlm.nih.gov/pubmed/21186054 http://www.sciencedirect.com/science/article/B6TWB-51ST6V0-4/2/93fada36d0fba3cbb3bb39227dba8ee0},
	doi = {10.1016/j.biomaterials.2010.11.064},
	abstract = {Guanine-rich oligonucleotides, such as TG4T and TG5T, assemble into a tetramolecular quadruplexes with layers of G-quartets stabilized by coordination to monovalent cations. Association rates of the quadruplexes are extremely slow, likely owing to electrostatic repulsion among the four strands. We have shown that comb-type copolymers with a polycation backbone and abundant hydrophilic graft chains form water-soluble polyelectrolyte complexes with DNA and promote DNA hybridization. Here, we report the effect of cationic comb-type copolymers on the kinetics of tetramolecular quadruplex formation. The copolymer significantly increased the association rate of tetramolecular quadruplexes without altering kinetic effects of metal cations in quadruplex formation. Dissociation rates of the quadruplexes were also accelerated by the copolymer suggesting that the copolymer has chaperone-like activity that reduces the energy barriers associated with dissociation and re-assembly of base pairs. This hypothesis was further supported by the observation that the copolymer activated the strand exchange reaction between the quadruplex and a constituting single-stranded. © 2010 Elsevier Ltd.},
	number = {9},
	journal = {Biomaterials},
	author = {Moriyama, Rui and Shimada, Naohiko and Kano, Arihiro and Maruyama, Atsushi},
	month = mar,
	year = {2011},
	pmid = {21186054},
	note = {tex.ids= moriyamaDNAAssemblyReassembly2011, moriyamaDNAAssemblyReassembly2011a
ISBN: 1878-5905 (Electronic)0̊142-9612 (Linking)
publisher: Elsevier Ltd},
	keywords = {Cationic comb-type copolymer, Cationic comb-type copolymer G-quadruplex Interpol, Cations, DNA, DNA: chemistry, DNA: metabolism, Dextrans, Dextrans: chemistry, Fluorescence, G-Quadruplexes, G-quadruplex, Interpolyelectrolyte complex, Kinetics, Nucleic Acid Denaturation, Nucleic Acid Denaturation: drug effects, Polylysine, Polylysine: pharmacology, Polymers, Polymers: pharmacology, Spectrometry, Strand exchange reaction, Temperature},
	pages = {2351--2358},
}

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We have shown that comb-type copolymers with a polycation backbone and abundant hydrophilic graft chains form water-soluble polyelectrolyte complexes with DNA and promote DNA hybridization. Here, we report the effect of cationic comb-type copolymers on the kinetics of tetramolecular quadruplex formation. The copolymer significantly increased the association rate of tetramolecular quadruplexes without altering kinetic effects of metal cations in quadruplex formation. Dissociation rates of the quadruplexes were also accelerated by the copolymer suggesting that the copolymer has chaperone-like activity that reduces the energy barriers associated with dissociation and re-assembly of base pairs. This hypothesis was further supported by the observation that the copolymer activated the strand exchange reaction between the quadruplex and a constituting single-stranded. © 2010 Elsevier Ltd.","number":"9","journal":"Biomaterials","author":[{"propositions":[],"lastnames":["Moriyama"],"firstnames":["Rui"],"suffixes":[]},{"propositions":[],"lastnames":["Shimada"],"firstnames":["Naohiko"],"suffixes":[]},{"propositions":[],"lastnames":["Kano"],"firstnames":["Arihiro"],"suffixes":[]},{"propositions":[],"lastnames":["Maruyama"],"firstnames":["Atsushi"],"suffixes":[]}],"month":"March","year":"2011","pmid":"21186054","note":"tex.ids= moriyamaDNAAssemblyReassembly2011, moriyamaDNAAssemblyReassembly2011a ISBN: 1878-5905 (Electronic)0̊142-9612 (Linking) publisher: Elsevier Ltd","keywords":"Cationic comb-type copolymer, Cationic comb-type copolymer G-quadruplex Interpol, Cations, DNA, DNA: chemistry, DNA: metabolism, Dextrans, Dextrans: chemistry, Fluorescence, G-Quadruplexes, G-quadruplex, Interpolyelectrolyte complex, Kinetics, Nucleic Acid Denaturation, Nucleic Acid Denaturation: drug effects, Polylysine, Polylysine: pharmacology, Polymers, Polymers: pharmacology, Spectrometry, Strand exchange reaction, Temperature","pages":"2351–2358","bibtex":"@article{Moriyama2011,\n\ttitle = {{DNA} assembly and re-assembly activated by cationic comb-type copolymer},\n\tvolume = {32},\n\tissn = {01429612},\n\turl = {http://dx.doi.org/10.1016/j.biomaterials.2010.11.064 http://www.ncbi.nlm.nih.gov/pubmed/21186054 http://www.sciencedirect.com/science/article/B6TWB-51ST6V0-4/2/93fada36d0fba3cbb3bb39227dba8ee0},\n\tdoi = {10.1016/j.biomaterials.2010.11.064},\n\tabstract = {Guanine-rich oligonucleotides, such as TG4T and TG5T, assemble into a tetramolecular quadruplexes with layers of G-quartets stabilized by coordination to monovalent cations. Association rates of the quadruplexes are extremely slow, likely owing to electrostatic repulsion among the four strands. We have shown that comb-type copolymers with a polycation backbone and abundant hydrophilic graft chains form water-soluble polyelectrolyte complexes with DNA and promote DNA hybridization. Here, we report the effect of cationic comb-type copolymers on the kinetics of tetramolecular quadruplex formation. The copolymer significantly increased the association rate of tetramolecular quadruplexes without altering kinetic effects of metal cations in quadruplex formation. Dissociation rates of the quadruplexes were also accelerated by the copolymer suggesting that the copolymer has chaperone-like activity that reduces the energy barriers associated with dissociation and re-assembly of base pairs. This hypothesis was further supported by the observation that the copolymer activated the strand exchange reaction between the quadruplex and a constituting single-stranded. © 2010 Elsevier Ltd.},\n\tnumber = {9},\n\tjournal = {Biomaterials},\n\tauthor = {Moriyama, Rui and Shimada, Naohiko and Kano, Arihiro and Maruyama, Atsushi},\n\tmonth = mar,\n\tyear = {2011},\n\tpmid = {21186054},\n\tnote = {tex.ids= moriyamaDNAAssemblyReassembly2011, moriyamaDNAAssemblyReassembly2011a\nISBN: 1878-5905 (Electronic)0̊142-9612 (Linking)\npublisher: Elsevier Ltd},\n\tkeywords = {Cationic comb-type copolymer, Cationic comb-type copolymer G-quadruplex Interpol, Cations, DNA, DNA: chemistry, DNA: metabolism, Dextrans, Dextrans: chemistry, Fluorescence, G-Quadruplexes, G-quadruplex, Interpolyelectrolyte complex, Kinetics, Nucleic Acid Denaturation, Nucleic Acid Denaturation: drug effects, Polylysine, Polylysine: pharmacology, Polymers, Polymers: pharmacology, Spectrometry, Strand exchange reaction, Temperature},\n\tpages = {2351--2358},\n}\n\n","author_short":["Moriyama, R.","Shimada, N.","Kano, A.","Maruyama, A."],"key":"Moriyama2011-1","id":"Moriyama2011-1","bibbaseid":"moriyama-shimada-kano-maruyama-dnaassemblyandreassemblyactivatedbycationiccombtypecopolymer-2011","role":"author","urls":{"Paper":"http://dx.doi.org/10.1016/j.biomaterials.2010.11.064 http://www.ncbi.nlm.nih.gov/pubmed/21186054 http://www.sciencedirect.com/science/article/B6TWB-51ST6V0-4/2/93fada36d0fba3cbb3bb39227dba8ee0"},"keyword":["Cationic comb-type copolymer","Cationic comb-type copolymer G-quadruplex Interpol","Cations","DNA","DNA: chemistry","DNA: metabolism","Dextrans","Dextrans: chemistry","Fluorescence","G-Quadruplexes","G-quadruplex","Interpolyelectrolyte complex","Kinetics","Nucleic Acid Denaturation","Nucleic Acid Denaturation: drug effects","Polylysine","Polylysine: pharmacology","Polymers","Polymers: pharmacology","Spectrometry","Strand exchange reaction","Temperature"],"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/zotero/eric.larG4","dataSources":["4i5C7S78DvJNsaHyg","5L2zM5wNE5CBYNuea"],"keywords":["cationic comb-type copolymer","cationic comb-type copolymer g-quadruplex interpol","cations","dna","dna: chemistry","dna: metabolism","dextrans","dextrans: chemistry","fluorescence","g-quadruplexes","g-quadruplex","interpolyelectrolyte complex","kinetics","nucleic acid denaturation","nucleic acid denaturation: drug effects","polylysine","polylysine: pharmacology","polymers","polymers: pharmacology","spectrometry","strand exchange reaction","temperature"],"search_terms":["dna","assembly","assembly","activated","cationic","comb","type","copolymer","moriyama","shimada","kano","maruyama"],"title":"DNA assembly and re-assembly activated by cationic comb-type copolymer","year":2011}