Effects of freeze-thaw stress on bacterial populations in soil microcosms. Morley, C. R., Trofymow, J. A., Coleman, D. C., & Cambardella, C. Microbial Ecology, 9(4):329–340, December, 1983.
Effects of freeze-thaw stress on bacterial populations in soil microcosms [link]Paper  doi  abstract   bibtex   
To test the effect of freezing on soil biota, isolated from the shortgrass prairie of northeastern Colorado, a series of experiments were performed using gnotobiotic soil microcosms.Pseudomonas paucimobilis was used to examine the effects of freezing on bacteria of different growth stages. Secondly, the effect of multiple freeze-thaw cycles was tested on an assemblage of bacterial species. Lastly, the effect of freezing on predator-prey interactions was studied usingP. paucimobilis and an amoebal predator,Acanthamoeba polyphaga. A temperature of −9°C was not detrimental toP. paucimobilis at any growth stage. A single severe freeze-thaw cycle (−27°C to 23°C) resulted in 40–60% mortality ofP. paucimobilis and the mixed bacteria, although additional freezing events did not reduce the populations further. Multiple freeze-thaw cycles (−9°C to 23°C) gave 40–60% mortality ofP. paucimobilis and the mixed bacteria. Predator-prey population cycles were possibly desynchronized by freeze-thaw events.
@article{morley_effects_1983,
	title = {Effects of freeze-thaw stress on bacterial populations in soil microcosms},
	volume = {9},
	issn = {1432-184X},
	url = {https://doi.org/10.1007/BF02019022},
	doi = {10.1007/BF02019022},
	abstract = {To test the effect of freezing on soil biota, isolated from the shortgrass prairie of northeastern Colorado, a series of experiments were performed using gnotobiotic soil microcosms.Pseudomonas paucimobilis was used to examine the effects of freezing on bacteria of different growth stages. Secondly, the effect of multiple freeze-thaw cycles was tested on an assemblage of bacterial species. Lastly, the effect of freezing on predator-prey interactions was studied usingP. paucimobilis and an amoebal predator,Acanthamoeba polyphaga. A temperature of −9°C was not detrimental toP. paucimobilis at any growth stage. A single severe freeze-thaw cycle (−27°C to 23°C) resulted in 40–60\% mortality ofP. paucimobilis and the mixed bacteria, although additional freezing events did not reduce the populations further. Multiple freeze-thaw cycles (−9°C to 23°C) gave 40–60\% mortality ofP. paucimobilis and the mixed bacteria. Predator-prey population cycles were possibly desynchronized by freeze-thaw events.},
	language = {en},
	number = {4},
	urldate = {2023-04-04},
	journal = {Microbial Ecology},
	author = {Morley, C. R. and Trofymow, J. A. and Coleman, D. C. and Cambardella, C.},
	month = dec,
	year = {1983},
	keywords = {Bacterial Population, Bacterial Species, Growth Stage, Nature Conservation, Pseudomonas},
	pages = {329--340},
}

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