Coupling two-step laser desorption/ionization with aerosol time-of-flight mass spectrometry for the analysis of individual organic particles. Morrical, B., D., Fergenson, D., P., & Prather, K., a. Journal Of The American Society For Mass Spectrometry, 9(10):1068-1073, 1998.
Coupling two-step laser desorption/ionization with aerosol time-of-flight mass spectrometry for the analysis of individual organic particles [pdf]Paper  abstract   bibtex   
Analysis of organic compounds in aerosol particles using real-time single Fat-tide techniques is difficult because of extensive fragmentation that occurs in the laser desorption/ionization step. In an effort to avoid such fragmentation processes, we coupled a "soft" two-step laser desorption/ionization technique (L2MS) with aerosol time-of-flight mass spectrometry (AT-OFMS). Ln these studies, we find this combination preserves intact organic molecules while providing the real-time mass spectra of suspended aerosol particles. Mass spectra of particles analyzed by one-step desorption mass spectrometry and L2MS are presented for comparison. These include 2,4-dihydroxybenzoic acid as a test case and wood and cigarette combustion particles as real world examples. This is the first published demonstration of L2MS performed on single particles not deposited on a substrate prior to analysis. (C) 1998 American Society for Mass Spectrometry.

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