Food resources of stream macroinvertebrates determined by natural-abundance stable C and N isotopes and a 15N tracer addition. Mulholland, P. J., Tank, J., Sanzone, D. M., Wollheim, W. M., Peterson, B., Webster, J., & Meyer, J. Journal North America Benthological Society, 2000. Paper abstract bibtex Trophic relationships were examined using natural-abundance 13C and 15N analyses and a 15N-tracer addition experiment in Walker Branch, a 1st-order forested stream in eastern Tennessee. In the 15N-tracer addition experiment, we added 15N: 14N ratios in different taxa and biomass compartments over distance and time. Samples collected from a station upstream from the 15N addition provided data on natural-abundance 13C: 14N ratios. The natural-abundance 15N analysis proved to be of limited value in identifying food resources of macroinvertebrates because 15N values were not greatly different among food resources, In general, the natural-abundance stable isotope approach was most useful for determining whether epilithon or detritus were important food resources for organisms that may use both (e.g., the snail Elimia clavaeformis), and to provide corroborative evidence of food resources of taxa for which the 15 N tracer results were not definitive. The 15N tracer results showed that the mayflies Stenonema supp. And Baetis spp. assimilated primarily epilithon, although Baetis spp. assimilated primarily epilithon, although Baetis appeared to assimilate a portion of the epilithon (e.g., algal cells) with more rapid N turnover than the bulk pool sampled. Although Elimia did not reach isotopic equilibrium during the tracer experiment, application of a N-turnover model to the field data suggested that it assimilated a combination of epilithon and detritus. The amphipod Gammarus minus appeared to depend mostly on fine benthic organic matter (FBOM), and the coleopteran Anchytarsus bicolor on epixylon. The caddisfly Diplectroma modesta appeared to assimilate primarily a fast N-turnover portion of the FBOM pool, and Simuliidae a fast N-turnover component of the suspended particulate organic matter pool rather than the bulk pool sampled. Together, the natural-abundance stable C and N isotope analyses and the experimental 15N tracer approach proved to be very useful tools for identifying food resources in this stream ecosystem.
@article{mulholland_food_2000,
title = {Food resources of stream macroinvertebrates determined by natural-abundance stable {C} and {N} isotopes and a 15N tracer addition.},
volume = {19},
url = {http://cwt33.ecology.uga.edu/publications/1400.pdf},
abstract = {Trophic relationships were examined using natural-abundance 13C and 15N analyses and a 15N-tracer addition experiment in Walker Branch, a 1st-order forested stream in eastern Tennessee. In the 15N-tracer addition experiment, we added 15N: 14N ratios in different taxa and biomass compartments over distance and time. Samples collected from a station upstream from the 15N addition provided data on natural-abundance 13C: 14N ratios. The natural-abundance 15N analysis proved to be of limited value in identifying food resources of macroinvertebrates because 15N values were not greatly different among food resources, In general, the natural-abundance stable isotope approach was most useful for determining whether epilithon or detritus were important food resources for organisms that may use both (e.g., the snail Elimia clavaeformis), and to provide corroborative evidence of food resources of taxa for which the 15 N tracer results were not definitive. The 15N tracer results showed that the mayflies Stenonema supp. And Baetis spp. assimilated primarily epilithon, although Baetis spp. assimilated primarily epilithon, although Baetis appeared to assimilate a portion of the epilithon (e.g., algal cells) with more rapid N turnover than the bulk pool sampled. Although Elimia did not reach isotopic equilibrium during the tracer experiment, application of a N-turnover model to the field data suggested that it assimilated a combination of epilithon and detritus. The amphipod Gammarus minus appeared to depend mostly on fine benthic organic matter (FBOM), and the coleopteran Anchytarsus bicolor on epixylon. The caddisfly Diplectroma modesta appeared to assimilate primarily a fast N-turnover portion of the FBOM pool, and Simuliidae a fast N-turnover component of the suspended particulate organic matter pool rather than the bulk pool sampled. Together, the natural-abundance stable C and N isotope analyses and the experimental 15N tracer approach proved to be very useful tools for identifying food resources in this stream ecosystem.},
number = {1},
journal = {Journal North America Benthological Society},
author = {Mulholland, Patrick J. and Tank, J.L. and Sanzone, D. M. and Wollheim, Wilfred M. and Peterson, B.J. and Webster, J.R. and Meyer, J.L.},
year = {2000},
keywords = {CWT}
}
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M.","Peterson, B.","Webster, J.","Meyer, J."],"year":2000,"bibtype":"article","biburl":"https://utexas.box.com/shared/static/1aa39ptglchcfuw9c04ozm0pqjlxu4rw.bib","bibdata":{"bibtype":"article","type":"article","title":"Food resources of stream macroinvertebrates determined by natural-abundance stable C and N isotopes and a 15N tracer addition.","volume":"19","url":"http://cwt33.ecology.uga.edu/publications/1400.pdf","abstract":"Trophic relationships were examined using natural-abundance 13C and 15N analyses and a 15N-tracer addition experiment in Walker Branch, a 1st-order forested stream in eastern Tennessee. In the 15N-tracer addition experiment, we added 15N: 14N ratios in different taxa and biomass compartments over distance and time. Samples collected from a station upstream from the 15N addition provided data on natural-abundance 13C: 14N ratios. The natural-abundance 15N analysis proved to be of limited value in identifying food resources of macroinvertebrates because 15N values were not greatly different among food resources, In general, the natural-abundance stable isotope approach was most useful for determining whether epilithon or detritus were important food resources for organisms that may use both (e.g., the snail Elimia clavaeformis), and to provide corroborative evidence of food resources of taxa for which the 15 N tracer results were not definitive. The 15N tracer results showed that the mayflies Stenonema supp. And Baetis spp. assimilated primarily epilithon, although Baetis spp. assimilated primarily epilithon, although Baetis appeared to assimilate a portion of the epilithon (e.g., algal cells) with more rapid N turnover than the bulk pool sampled. Although Elimia did not reach isotopic equilibrium during the tracer experiment, application of a N-turnover model to the field data suggested that it assimilated a combination of epilithon and detritus. The amphipod Gammarus minus appeared to depend mostly on fine benthic organic matter (FBOM), and the coleopteran Anchytarsus bicolor on epixylon. The caddisfly Diplectroma modesta appeared to assimilate primarily a fast N-turnover portion of the FBOM pool, and Simuliidae a fast N-turnover component of the suspended particulate organic matter pool rather than the bulk pool sampled. 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The 15N tracer results showed that the mayflies Stenonema supp. And Baetis spp. assimilated primarily epilithon, although Baetis spp. assimilated primarily epilithon, although Baetis appeared to assimilate a portion of the epilithon (e.g., algal cells) with more rapid N turnover than the bulk pool sampled. Although Elimia did not reach isotopic equilibrium during the tracer experiment, application of a N-turnover model to the field data suggested that it assimilated a combination of epilithon and detritus. The amphipod Gammarus minus appeared to depend mostly on fine benthic organic matter (FBOM), and the coleopteran Anchytarsus bicolor on epixylon. The caddisfly Diplectroma modesta appeared to assimilate primarily a fast N-turnover portion of the FBOM pool, and Simuliidae a fast N-turnover component of the suspended particulate organic matter pool rather than the bulk pool sampled. 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