Factors influencing heat-labile Escherichia coli enterotoxin activity. Mundell, D. H., Anselmo, C. R., & Wishnow, R. M. Infection and Immunity, 14(2):383--388, August, 1976. ![Factors influencing heat-labile Escherichia coli enterotoxin activity [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper abstract bibtex In this study, conditions for production, detection, and storage of heat-labile Escherichia coli enterotoxin (LT) in culture filtrates from E. coli H-10407 were defined by using the adrenal tumor cell assay system. An enriched medium containing 0.6% yeast extract, 2% Casamino Acids, and 0.25% glucose buffered at pH 8.5 produced the highest LT activity of the various test media. In E. coli strain H-10407, LT activity was markedly decreased if the initial pH of the culture media was reduced to pH 7.5 or less. In contrast to E. coli P-263, if strain H-10407 was grown in the presence of mitomycin C there was no increase in LT production. Crude-culture filtrates containing LT can be stored at 4 degrees C for several days without an appreciable loss of activity; however, for long-term storage lyophilization or freezing at -70 degrees C is recommended.
@article{mundell_factors_1976,
title = {Factors influencing heat-labile {Escherichia} coli enterotoxin activity},
volume = {14},
issn = {0019-9567},
url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC420895/},
abstract = {In this study, conditions for production, detection, and storage of heat-labile Escherichia coli enterotoxin (LT) in culture filtrates from E. coli H-10407 were defined by using the adrenal tumor cell assay system. An enriched medium containing 0.6\% yeast extract, 2\% Casamino Acids, and 0.25\% glucose buffered at pH 8.5 produced the highest LT activity of the various test media. In E. coli strain H-10407, LT activity was markedly decreased if the initial pH of the culture media was reduced to pH 7.5 or less. In contrast to E. coli P-263, if strain H-10407 was grown in the presence of mitomycin C there was no increase in LT production. Crude-culture filtrates containing LT can be stored at 4 degrees C for several days without an appreciable loss of activity; however, for long-term storage lyophilization or freezing at -70 degrees C is recommended.},
language = {ENG},
number = {2},
journal = {Infection and Immunity},
author = {Mundell, D. H. and Anselmo, C. R. and Wishnow, R. M.},
month = aug,
year = {1976},
pmid = {9363},
pmcid = {PMC420895},
keywords = {Culture Media, Enterotoxins, Escherichia coli, Hot Temperature, Hydrogen-Ion Concentration, Mitomycins, Temperature, Time Factors},
pages = {383--388}
}
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