Modeling of Transmembrane Potential in Realistic Multicellular Structures before Electroporation. Murovec, T., Sweeney, D. C., Latouche, E., Davalos, R. V., & Brosseau, C. Biophys J, 111(10):2286-2295, 2016. 1542-0086 Murovec, Tomo Sweeney, Daniel C Latouche, Eduardo Davalos, Rafael V Brosseau, Christian R21 CA173092/CA/NCI NIH HHS/United States Journal Article United States 2016/11/17 Biophys J. 2016 Nov 15;111(10):2286-2295. doi: 10.1016/j.bpj.2016.10.005.doi abstract bibtex Many approaches for studying the transmembrane potential (TMP) induced during the treatment of biological cells with pulsed electric fields have been reported. From the simple analytical models to more complex numerical models requiring significant computational resources, a gamut of methods have been used to recapitulate multicellular environments in silico. Cells have been modeled as simple shapes in two dimensions as well as more complex geometries attempting to replicate realistic cell shapes. In this study, we describe a method for extracting realistic cell morphologies from fluorescence microscopy images to generate the piecewise continuous mesh used to develop a finite element model in two dimensions. The preelectroporation TMP induced in tightly packed cells is analyzed for two sets of pulse parameters inspired by clinical irreversible electroporation treatments. We show that high-frequency bipolar pulse trains are better, and more homogeneously raise the TMP of tightly packed cells to a simulated electroporation threshold than conventional irreversible electroporation pulse trains, at the expense of larger applied potentials. Our results demonstrate the viability of our method and emphasize the importance of considering multicellular effects in the numerical models used for studying the response of biological tissues exposed to electric fields.
@article{RN172,
author = {Murovec, T. and Sweeney, D. C. and Latouche, E. and Davalos, R. V. and Brosseau, C.},
title = {Modeling of Transmembrane Potential in Realistic Multicellular Structures before Electroporation},
journal = {Biophys J},
volume = {111},
number = {10},
pages = {2286-2295},
note = {1542-0086
Murovec, Tomo
Sweeney, Daniel C
Latouche, Eduardo
Davalos, Rafael V
Brosseau, Christian
R21 CA173092/CA/NCI NIH HHS/United States
Journal Article
United States
2016/11/17
Biophys J. 2016 Nov 15;111(10):2286-2295. doi: 10.1016/j.bpj.2016.10.005.},
abstract = {Many approaches for studying the transmembrane potential (TMP) induced during the treatment of biological cells with pulsed electric fields have been reported. From the simple analytical models to more complex numerical models requiring significant computational resources, a gamut of methods have been used to recapitulate multicellular environments in silico. Cells have been modeled as simple shapes in two dimensions as well as more complex geometries attempting to replicate realistic cell shapes. In this study, we describe a method for extracting realistic cell morphologies from fluorescence microscopy images to generate the piecewise continuous mesh used to develop a finite element model in two dimensions. The preelectroporation TMP induced in tightly packed cells is analyzed for two sets of pulse parameters inspired by clinical irreversible electroporation treatments. We show that high-frequency bipolar pulse trains are better, and more homogeneously raise the TMP of tightly packed cells to a simulated electroporation threshold than conventional irreversible electroporation pulse trains, at the expense of larger applied potentials. Our results demonstrate the viability of our method and emphasize the importance of considering multicellular effects in the numerical models used for studying the response of biological tissues exposed to electric fields.},
keywords = {Animals
*Electroporation
Finite Element Analysis
*Membrane Potentials
Mice
Microscopy, Fluorescence
*Models, Biological},
ISSN = {0006-3495 (Print)
0006-3495},
DOI = {10.1016/j.bpj.2016.10.005},
year = {2016},
type = {Journal Article}
}
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V. and Brosseau, C.},\n title = {Modeling of Transmembrane Potential in Realistic Multicellular Structures before Electroporation},\n journal = {Biophys J},\n volume = {111},\n number = {10},\n pages = {2286-2295},\n note = {1542-0086\nMurovec, Tomo\nSweeney, Daniel C\nLatouche, Eduardo\nDavalos, Rafael V\nBrosseau, Christian\nR21 CA173092/CA/NCI NIH HHS/United States\nJournal Article\nUnited States\n2016/11/17\nBiophys J. 2016 Nov 15;111(10):2286-2295. doi: 10.1016/j.bpj.2016.10.005.},\n abstract = {Many approaches for studying the transmembrane potential (TMP) induced during the treatment of biological cells with pulsed electric fields have been reported. From the simple analytical models to more complex numerical models requiring significant computational resources, a gamut of methods have been used to recapitulate multicellular environments in silico. 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