@article{RN113,
   author = {Murphy, K. R. and Aycock, K. N. and Hay, A. N. and Rossmeisl, J. H. and Davalos, R. V. and Dervisis, N. G.},
   title = {High-frequency irreversible electroporation brain tumor ablation: exploring the dynamics of cell death and recovery},
   journal = {Bioelectrochemistry},
   volume = {144},
   pages = {108001},
   note = {1878-562x
Murphy, Kelsey R
Aycock, Kenneth N
Hay, Alayna N
Rossmeisl, John H
Davalos, Rafael V
Dervisis, Nikolaos G
R01 CA213423/CA/NCI NIH HHS/United States
R01 CA240476/CA/NCI NIH HHS/United States
Journal Article
Netherlands
2021/11/30
Bioelectrochemistry. 2022 Apr;144:108001. doi: 10.1016/j.bioelechem.2021.108001. Epub 2021 Nov 17.},
   abstract = {Improved therapeutics for malignant brain tumors are urgently needed. High-frequency irreversible electroporation (H-FIRE) is a minimally invasive, nonthermal tissue ablation technique, which utilizes high-frequency, bipolar electric pulses to precisely kill tumor cells. The mechanisms of H-FIRE-induced tumor cell death and potential for cellular recovery are incompletely characterized. We hypothesized that tumor cells treated with specific H-FIRE electric field doses can survive and retain proliferative capacity. F98 glioma and LL/2 Lewis lung carcinoma cell suspensions were treated with H-FIRE to model primary and metastatic brain cancer, respectively. Cell membrane permeability, apoptosis, metabolic viability, and proliferative capacity were temporally measured using exclusion dyes, condensed chromatin staining, WST-8 fluorescence, and clonogenic assays, respectively. Both tumor cell lines exhibited dose-dependent permeabilization, with 1,500 V/cm permitting and 3,000 V/cm inhibiting membrane recovery 24 h post-treatment. Cells treated with 1,500 V/cm demonstrated significant and progressive recovery of apoptosis and metabolic activity, in contrast to cells treated with higher H-FIRE doses. Cancer cells treated with recovery-permitting doses of H-FIRE maintained while those treated with recovery-inhibiting doses lost proliferative capacity. Taken together, our data suggest that H-FIRE induces reversible and irreversible cellular damage in a dose-dependent manner, and the presence of dose-dependent recovery mechanisms permits tumor cell proliferation.},
   keywords = {*Brain Neoplasms
Apoptosis
Brain cancer
Cell death
High-frequency irreversible electroporation
Proliferation
Recovery},
   ISSN = {1567-5394 (Print)
1567-5394},
   DOI = {10.1016/j.bioelechem.2021.108001},
   year = {2022},
   type = {Journal Article}
}

{"_id":"5889pQjEsPKtiRdgv","bibbaseid":"murphy-aycock-hay-rossmeisl-davalos-dervisis-highfrequencyirreversibleelectroporationbraintumorablationexploringthedynamicsofcelldeathandrecovery-2022","author_short":["Murphy, K. R.","Aycock, K. N.","Hay, A. N.","Rossmeisl, J. H.","Davalos, R. V.","Dervisis, N. G."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Murphy"],"firstnames":["K.","R."],"suffixes":[]},{"propositions":[],"lastnames":["Aycock"],"firstnames":["K.","N."],"suffixes":[]},{"propositions":[],"lastnames":["Hay"],"firstnames":["A.","N."],"suffixes":[]},{"propositions":[],"lastnames":["Rossmeisl"],"firstnames":["J.","H."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]},{"propositions":[],"lastnames":["Dervisis"],"firstnames":["N.","G."],"suffixes":[]}],"title":"High-frequency irreversible electroporation brain tumor ablation: exploring the dynamics of cell death and recovery","journal":"Bioelectrochemistry","volume":"144","pages":"108001","note":"1878-562x Murphy, Kelsey R Aycock, Kenneth N Hay, Alayna N Rossmeisl, John H Davalos, Rafael V Dervisis, Nikolaos G R01 CA213423/CA/NCI NIH HHS/United States R01 CA240476/CA/NCI NIH HHS/United States Journal Article Netherlands 2021/11/30 Bioelectrochemistry. 2022 Apr;144:108001. doi: 10.1016/j.bioelechem.2021.108001. Epub 2021 Nov 17.","abstract":"Improved therapeutics for malignant brain tumors are urgently needed. High-frequency irreversible electroporation (H-FIRE) is a minimally invasive, nonthermal tissue ablation technique, which utilizes high-frequency, bipolar electric pulses to precisely kill tumor cells. The mechanisms of H-FIRE-induced tumor cell death and potential for cellular recovery are incompletely characterized. We hypothesized that tumor cells treated with specific H-FIRE electric field doses can survive and retain proliferative capacity. F98 glioma and LL/2 Lewis lung carcinoma cell suspensions were treated with H-FIRE to model primary and metastatic brain cancer, respectively. Cell membrane permeability, apoptosis, metabolic viability, and proliferative capacity were temporally measured using exclusion dyes, condensed chromatin staining, WST-8 fluorescence, and clonogenic assays, respectively. Both tumor cell lines exhibited dose-dependent permeabilization, with 1,500 V/cm permitting and 3,000 V/cm inhibiting membrane recovery 24 h post-treatment. Cells treated with 1,500 V/cm demonstrated significant and progressive recovery of apoptosis and metabolic activity, in contrast to cells treated with higher H-FIRE doses. Cancer cells treated with recovery-permitting doses of H-FIRE maintained while those treated with recovery-inhibiting doses lost proliferative capacity. Taken together, our data suggest that H-FIRE induces reversible and irreversible cellular damage in a dose-dependent manner, and the presence of dose-dependent recovery mechanisms permits tumor cell proliferation.","keywords":"*Brain Neoplasms Apoptosis Brain cancer Cell death High-frequency irreversible electroporation Proliferation Recovery","issn":"1567-5394 (Print) 1567-5394","doi":"10.1016/j.bioelechem.2021.108001","year":"2022","bibtex":"@article{RN113,\n author = {Murphy, K. R. and Aycock, K. N. and Hay, A. N. and Rossmeisl, J. H. and Davalos, R. V. and Dervisis, N. G.},\n title = {High-frequency irreversible electroporation brain tumor ablation: exploring the dynamics of cell death and recovery},\n journal = {Bioelectrochemistry},\n volume = {144},\n pages = {108001},\n note = {1878-562x\nMurphy, Kelsey R\nAycock, Kenneth N\nHay, Alayna N\nRossmeisl, John H\nDavalos, Rafael V\nDervisis, Nikolaos G\nR01 CA213423/CA/NCI NIH HHS/United States\nR01 CA240476/CA/NCI NIH HHS/United States\nJournal Article\nNetherlands\n2021/11/30\nBioelectrochemistry. 2022 Apr;144:108001. doi: 10.1016/j.bioelechem.2021.108001. Epub 2021 Nov 17.},\n abstract = {Improved therapeutics for malignant brain tumors are urgently needed. High-frequency irreversible electroporation (H-FIRE) is a minimally invasive, nonthermal tissue ablation technique, which utilizes high-frequency, bipolar electric pulses to precisely kill tumor cells. The mechanisms of H-FIRE-induced tumor cell death and potential for cellular recovery are incompletely characterized. We hypothesized that tumor cells treated with specific H-FIRE electric field doses can survive and retain proliferative capacity. F98 glioma and LL/2 Lewis lung carcinoma cell suspensions were treated with H-FIRE to model primary and metastatic brain cancer, respectively. Cell membrane permeability, apoptosis, metabolic viability, and proliferative capacity were temporally measured using exclusion dyes, condensed chromatin staining, WST-8 fluorescence, and clonogenic assays, respectively. Both tumor cell lines exhibited dose-dependent permeabilization, with 1,500 V/cm permitting and 3,000 V/cm inhibiting membrane recovery 24 h post-treatment. Cells treated with 1,500 V/cm demonstrated significant and progressive recovery of apoptosis and metabolic activity, in contrast to cells treated with higher H-FIRE doses. Cancer cells treated with recovery-permitting doses of H-FIRE maintained while those treated with recovery-inhibiting doses lost proliferative capacity. Taken together, our data suggest that H-FIRE induces reversible and irreversible cellular damage in a dose-dependent manner, and the presence of dose-dependent recovery mechanisms permits tumor cell proliferation.},\n keywords = {*Brain Neoplasms\nApoptosis\nBrain cancer\nCell death\nHigh-frequency irreversible electroporation\nProliferation\nRecovery},\n ISSN = {1567-5394 (Print)\n1567-5394},\n DOI = {10.1016/j.bioelechem.2021.108001},\n year = {2022},\n type = {Journal Article}\n}\n\n","author_short":["Murphy, K. R.","Aycock, K. N.","Hay, A. N.","Rossmeisl, J. H.","Davalos, R. V.","Dervisis, N. G."],"key":"RN113","id":"RN113","bibbaseid":"murphy-aycock-hay-rossmeisl-davalos-dervisis-highfrequencyirreversibleelectroporationbraintumorablationexploringthedynamicsofcelldeathandrecovery-2022","role":"author","urls":{},"keyword":["*Brain Neoplasms Apoptosis Brain cancer Cell death High-frequency irreversible electroporation Proliferation Recovery"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","ZPLjameRikygaiM9B","3XfNmZkLe6o8CvECW","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["*brain neoplasms apoptosis brain cancer cell death high-frequency irreversible electroporation proliferation recovery"],"search_terms":["high","frequency","irreversible","electroporation","brain","tumor","ablation","exploring","dynamics","cell","death","recovery","murphy","aycock","hay","rossmeisl","davalos","dervisis"],"title":"High-frequency irreversible electroporation brain tumor ablation: exploring the dynamics of cell death and recovery","year":2022}