NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence. Nagahage, I. S. P., Matsuda, K., Miyashita, K., Fujiwara, S., Mannapperuma, C., Yamada, T., Sakamoto, S., Ishikawa, T., Nagano, M., Ohtani, M., Kato, K., Uchimiya, H., Mitsuda, N., Kawai-Yamada, M., Demura, T., & Yamaguchi, M. Plant Direct, 7(9):e529, September, 2023. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/pld3.529![NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND-INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR-RELATED NAC-DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacting proteins with VNI2 using yeast two-hybrid assay and isolated two NAC domain transcription factors, Arabidopsis thaliana ACTIVATION FACTOR 2 (ATAF2) and NAC DOMAIN CONTAINING PROTEIN 102 (ANAC102). A transient gene expression assay showed that ATAF2 upregulates the expression of genes involved in leaf senescence, and VNI2 effectively inhibits the transcriptional activation activity of ATAF2. vni2 mutants accelerate leaf senescence, whereas ataf2 mutants delay leaf senescence. In addition, the accelerated leaf senescence phenotype of the vni2 mutant is recovered by simultaneous mutation of ATAF2. Our findings strongly suggest that VNI2 interacts with and inhibits ATAF2, resulting in negatively regulating leaf senescence.
@article{nagahage_nac_2023,
title = {{NAC} domain transcription factors {VNI2} and {ATAF2} form protein complexes and regulate leaf senescence},
volume = {7},
copyright = {© 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley \& Sons Ltd.},
issn = {2475-4455},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1002/pld3.529},
doi = {10.1002/pld3.529},
abstract = {The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND-INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR-RELATED NAC-DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacting proteins with VNI2 using yeast two-hybrid assay and isolated two NAC domain transcription factors, Arabidopsis thaliana ACTIVATION FACTOR 2 (ATAF2) and NAC DOMAIN CONTAINING PROTEIN 102 (ANAC102). A transient gene expression assay showed that ATAF2 upregulates the expression of genes involved in leaf senescence, and VNI2 effectively inhibits the transcriptional activation activity of ATAF2. vni2 mutants accelerate leaf senescence, whereas ataf2 mutants delay leaf senescence. In addition, the accelerated leaf senescence phenotype of the vni2 mutant is recovered by simultaneous mutation of ATAF2. Our findings strongly suggest that VNI2 interacts with and inhibits ATAF2, resulting in negatively regulating leaf senescence.},
language = {en},
number = {9},
urldate = {2023-09-22},
journal = {Plant Direct},
author = {Nagahage, Isura Sumeda Priyadarshana and Matsuda, Kohei and Miyashita, Kyoko and Fujiwara, Sumire and Mannapperuma, Chanaka and Yamada, Takuya and Sakamoto, Shingo and Ishikawa, Toshiki and Nagano, Minoru and Ohtani, Misato and Kato, Ko and Uchimiya, Hirofumi and Mitsuda, Nobutaka and Kawai-Yamada, Maki and Demura, Taku and Yamaguchi, Masatoshi},
month = sep,
year = {2023},
note = {\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/pld3.529},
keywords = {Arabidopsis thaliana, NAC domain protein, leaf senescence, protein–protein interaction, transcription factor},
pages = {e529},
}
Downloads: 0
{"_id":"AiePpZ7ZpRZcK3BuH","bibbaseid":"nagahage-matsuda-miyashita-fujiwara-mannapperuma-yamada-sakamoto-ishikawa-etal-nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence-2023","author_short":["Nagahage, I. S. P.","Matsuda, K.","Miyashita, K.","Fujiwara, S.","Mannapperuma, C.","Yamada, T.","Sakamoto, S.","Ishikawa, T.","Nagano, M.","Ohtani, M.","Kato, K.","Uchimiya, H.","Mitsuda, N.","Kawai-Yamada, M.","Demura, T.","Yamaguchi, M."],"bibdata":{"bibtype":"article","type":"article","title":"NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence","volume":"7","copyright":"© 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.","issn":"2475-4455","url":"https://onlinelibrary.wiley.com/doi/abs/10.1002/pld3.529","doi":"10.1002/pld3.529","abstract":"The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND-INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR-RELATED NAC-DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacting proteins with VNI2 using yeast two-hybrid assay and isolated two NAC domain transcription factors, Arabidopsis thaliana ACTIVATION FACTOR 2 (ATAF2) and NAC DOMAIN CONTAINING PROTEIN 102 (ANAC102). A transient gene expression assay showed that ATAF2 upregulates the expression of genes involved in leaf senescence, and VNI2 effectively inhibits the transcriptional activation activity of ATAF2. vni2 mutants accelerate leaf senescence, whereas ataf2 mutants delay leaf senescence. In addition, the accelerated leaf senescence phenotype of the vni2 mutant is recovered by simultaneous mutation of ATAF2. Our findings strongly suggest that VNI2 interacts with and inhibits ATAF2, resulting in negatively regulating leaf senescence.","language":"en","number":"9","urldate":"2023-09-22","journal":"Plant Direct","author":[{"propositions":[],"lastnames":["Nagahage"],"firstnames":["Isura","Sumeda","Priyadarshana"],"suffixes":[]},{"propositions":[],"lastnames":["Matsuda"],"firstnames":["Kohei"],"suffixes":[]},{"propositions":[],"lastnames":["Miyashita"],"firstnames":["Kyoko"],"suffixes":[]},{"propositions":[],"lastnames":["Fujiwara"],"firstnames":["Sumire"],"suffixes":[]},{"propositions":[],"lastnames":["Mannapperuma"],"firstnames":["Chanaka"],"suffixes":[]},{"propositions":[],"lastnames":["Yamada"],"firstnames":["Takuya"],"suffixes":[]},{"propositions":[],"lastnames":["Sakamoto"],"firstnames":["Shingo"],"suffixes":[]},{"propositions":[],"lastnames":["Ishikawa"],"firstnames":["Toshiki"],"suffixes":[]},{"propositions":[],"lastnames":["Nagano"],"firstnames":["Minoru"],"suffixes":[]},{"propositions":[],"lastnames":["Ohtani"],"firstnames":["Misato"],"suffixes":[]},{"propositions":[],"lastnames":["Kato"],"firstnames":["Ko"],"suffixes":[]},{"propositions":[],"lastnames":["Uchimiya"],"firstnames":["Hirofumi"],"suffixes":[]},{"propositions":[],"lastnames":["Mitsuda"],"firstnames":["Nobutaka"],"suffixes":[]},{"propositions":[],"lastnames":["Kawai-Yamada"],"firstnames":["Maki"],"suffixes":[]},{"propositions":[],"lastnames":["Demura"],"firstnames":["Taku"],"suffixes":[]},{"propositions":[],"lastnames":["Yamaguchi"],"firstnames":["Masatoshi"],"suffixes":[]}],"month":"September","year":"2023","note":"_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/pld3.529","keywords":"Arabidopsis thaliana, NAC domain protein, leaf senescence, protein–protein interaction, transcription factor","pages":"e529","bibtex":"@article{nagahage_nac_2023,\n\ttitle = {{NAC} domain transcription factors {VNI2} and {ATAF2} form protein complexes and regulate leaf senescence},\n\tvolume = {7},\n\tcopyright = {© 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley \\& Sons Ltd.},\n\tissn = {2475-4455},\n\turl = {https://onlinelibrary.wiley.com/doi/abs/10.1002/pld3.529},\n\tdoi = {10.1002/pld3.529},\n\tabstract = {The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND-INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR-RELATED NAC-DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacting proteins with VNI2 using yeast two-hybrid assay and isolated two NAC domain transcription factors, Arabidopsis thaliana ACTIVATION FACTOR 2 (ATAF2) and NAC DOMAIN CONTAINING PROTEIN 102 (ANAC102). A transient gene expression assay showed that ATAF2 upregulates the expression of genes involved in leaf senescence, and VNI2 effectively inhibits the transcriptional activation activity of ATAF2. vni2 mutants accelerate leaf senescence, whereas ataf2 mutants delay leaf senescence. In addition, the accelerated leaf senescence phenotype of the vni2 mutant is recovered by simultaneous mutation of ATAF2. Our findings strongly suggest that VNI2 interacts with and inhibits ATAF2, resulting in negatively regulating leaf senescence.},\n\tlanguage = {en},\n\tnumber = {9},\n\turldate = {2023-09-22},\n\tjournal = {Plant Direct},\n\tauthor = {Nagahage, Isura Sumeda Priyadarshana and Matsuda, Kohei and Miyashita, Kyoko and Fujiwara, Sumire and Mannapperuma, Chanaka and Yamada, Takuya and Sakamoto, Shingo and Ishikawa, Toshiki and Nagano, Minoru and Ohtani, Misato and Kato, Ko and Uchimiya, Hirofumi and Mitsuda, Nobutaka and Kawai-Yamada, Maki and Demura, Taku and Yamaguchi, Masatoshi},\n\tmonth = sep,\n\tyear = {2023},\n\tnote = {\\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/pld3.529},\n\tkeywords = {Arabidopsis thaliana, NAC domain protein, leaf senescence, protein–protein interaction, transcription factor},\n\tpages = {e529},\n}\n\n\n\n","author_short":["Nagahage, I. S. P.","Matsuda, K.","Miyashita, K.","Fujiwara, S.","Mannapperuma, C.","Yamada, T.","Sakamoto, S.","Ishikawa, T.","Nagano, M.","Ohtani, M.","Kato, K.","Uchimiya, H.","Mitsuda, N.","Kawai-Yamada, M.","Demura, T.","Yamaguchi, M."],"key":"nagahage_nac_2023","id":"nagahage_nac_2023","bibbaseid":"nagahage-matsuda-miyashita-fujiwara-mannapperuma-yamada-sakamoto-ishikawa-etal-nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence-2023","role":"author","urls":{"Paper":"https://onlinelibrary.wiley.com/doi/abs/10.1002/pld3.529"},"keyword":["Arabidopsis thaliana","NAC domain protein","leaf senescence","protein–protein interaction","transcription factor"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["3zTPPmKj8BiTcpc6C","9cGcv2t8pRzC92kzs"],"keywords":["arabidopsis thaliana","nac domain protein","leaf senescence","protein–protein interaction","transcription factor"],"search_terms":["nac","domain","transcription","factors","vni2","ataf2","form","protein","complexes","regulate","leaf","senescence","nagahage","matsuda","miyashita","fujiwara","mannapperuma","yamada","sakamoto","ishikawa","nagano","ohtani","kato","uchimiya","mitsuda","kawai-yamada","demura","yamaguchi"],"title":"NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence","year":2023}