Translation inhibition and resource balance in the TX-TL cell-free gene expression system. Nagaraj, V. H., Greene, J. M., Sengupta, A. M., , & Sontag, E. Synthetic Biology, 2:ysx005, 2017. abstract bibtex Utilizing the synthetic transcription-translation (TX-TL) system, this paper studies the impact of nucleotide triphosphates (NTPs) and magnesium (Mg2+), on gene expression, in the context of the counterintuitive phenomenon of suppression of gene expression at high NTP concentration. Measuring translation rates for different Mg2+ and NTP concentrations, we observe a complex resource dependence. We demonstrate that translation is the rate-limiting process that is directly inhibited by high NTP concentrations. Additional Mg2+ can partially reverse this inhibition. In several experiments, we observe two maxima of the translation rate viewed as a function of both Mg2+ and NTP concentration, which can be explained in terms of an NTP-independent effect on the ribosome complex and an NTP- Mg2+ titration effect. The non-trivial compensatory effects of abundance of different vital resources signals the presence of complex regulatory mechanisms to achieve optimal gene expression.
@ARTICLE{txtl2017,
AUTHOR = {V. H. Nagaraj and J. M. Greene and A. M. Sengupta and
and E.D. Sontag},
JOURNAL = {Synthetic Biology},
TITLE = {Translation inhibition and resource balance in the TX-TL
cell-free gene expression system},
YEAR = {2017},
OPTMONTH = {},
OPTNOTE = {},
OPTNUMBER = {},
PAGES = {ysx005},
VOLUME = {2},
KEYWORDS = {cell-free systems, in vitro synthetic biology},
PDF = {../../FTPDIR/nagaraj_greene_sengupta_sontag_reprint_2017.pdf},
ABSTRACT = {Utilizing the synthetic transcription-translation
(TX-TL) system, this paper studies the impact of nucleotide
triphosphates (NTPs) and magnesium (Mg2+), on gene expression, in the
context of the counterintuitive phenomenon of suppression of gene
expression at high NTP concentration. Measuring translation rates for
different Mg2+ and NTP concentrations, we observe a complex resource
dependence. We demonstrate that translation is the rate-limiting
process that is directly inhibited by high NTP concentrations.
Additional Mg2+ can partially reverse this inhibition. In several
experiments, we observe two maxima of the translation rate viewed as
a function of both Mg2+ and NTP concentration, which can be explained
in terms of an NTP-independent effect on the ribosome complex and an
NTP- Mg2+ titration effect. The non-trivial compensatory effects of
abundance of different vital resources signals the presence of
complex regulatory mechanisms to achieve optimal gene expression.}
}
Downloads: 0
{"_id":"ntWihEENCPFjAdSuh","bibbaseid":"nagaraj-greene-sengupta--sontag-translationinhibitionandresourcebalanceinthetxtlcellfreegeneexpressionsystem-2017","downloads":0,"creationDate":"2018-10-18T05:07:05.854Z","title":"Translation inhibition and resource balance in the TX-TL cell-free gene expression system","author_short":["Nagaraj, V. H.","Greene, J. M.","Sengupta, A. M.","","Sontag, E."],"year":2017,"bibtype":"article","biburl":"http://www.sontaglab.org/PUBDIR/Biblio/complete-bibliography.bib","bibdata":{"bibtype":"article","type":"article","author":[{"firstnames":["V.","H."],"propositions":[],"lastnames":["Nagaraj"],"suffixes":[]},{"firstnames":["J.","M."],"propositions":[],"lastnames":["Greene"],"suffixes":[]},{"firstnames":["A.","M."],"propositions":[],"lastnames":["Sengupta"],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":[""],"suffixes":[]},{"firstnames":["E.D."],"propositions":[],"lastnames":["Sontag"],"suffixes":[]}],"journal":"Synthetic Biology","title":"Translation inhibition and resource balance in the TX-TL cell-free gene expression system","year":"2017","optmonth":"","optnote":"","optnumber":"","pages":"ysx005","volume":"2","keywords":"cell-free systems, in vitro synthetic biology","pdf":"../../FTPDIR/nagaraj_greene_sengupta_sontag_reprint_2017.pdf","abstract":"Utilizing the synthetic transcription-translation (TX-TL) system, this paper studies the impact of nucleotide triphosphates (NTPs) and magnesium (Mg2+), on gene expression, in the context of the counterintuitive phenomenon of suppression of gene expression at high NTP concentration. Measuring translation rates for different Mg2+ and NTP concentrations, we observe a complex resource dependence. We demonstrate that translation is the rate-limiting process that is directly inhibited by high NTP concentrations. Additional Mg2+ can partially reverse this inhibition. In several experiments, we observe two maxima of the translation rate viewed as a function of both Mg2+ and NTP concentration, which can be explained in terms of an NTP-independent effect on the ribosome complex and an NTP- Mg2+ titration effect. The non-trivial compensatory effects of abundance of different vital resources signals the presence of complex regulatory mechanisms to achieve optimal gene expression.","bibtex":"@ARTICLE{txtl2017,\n AUTHOR = {V. H. Nagaraj and J. M. Greene and A. M. Sengupta and \n and E.D. Sontag},\n JOURNAL = {Synthetic Biology},\n TITLE = {Translation inhibition and resource balance in the TX-TL \n cell-free gene expression system},\n YEAR = {2017},\n OPTMONTH = {},\n OPTNOTE = {},\n OPTNUMBER = {},\n PAGES = {ysx005},\n VOLUME = {2},\n KEYWORDS = {cell-free systems, in vitro synthetic biology},\n PDF = {../../FTPDIR/nagaraj_greene_sengupta_sontag_reprint_2017.pdf},\n ABSTRACT = {Utilizing the synthetic transcription-translation \n (TX-TL) system, this paper studies the impact of nucleotide \n triphosphates (NTPs) and magnesium (Mg2+), on gene expression, in the \n context of the counterintuitive phenomenon of suppression of gene \n expression at high NTP concentration. Measuring translation rates for \n different Mg2+ and NTP concentrations, we observe a complex resource \n dependence. We demonstrate that translation is the rate-limiting \n process that is directly inhibited by high NTP concentrations. \n Additional Mg2+ can partially reverse this inhibition. In several \n experiments, we observe two maxima of the translation rate viewed as \n a function of both Mg2+ and NTP concentration, which can be explained \n in terms of an NTP-independent effect on the ribosome complex and an \n NTP- Mg2+ titration effect. The non-trivial compensatory effects of \n abundance of different vital resources signals the presence of \n complex regulatory mechanisms to achieve optimal gene expression.}\n}\n\n","author_short":["Nagaraj, V. H.","Greene, J. M.","Sengupta, A. M.","","Sontag, E."],"key":"txtl2017","id":"txtl2017","bibbaseid":"nagaraj-greene-sengupta--sontag-translationinhibitionandresourcebalanceinthetxtlcellfreegeneexpressionsystem-2017","role":"author","urls":{},"keyword":["cell-free systems","in vitro synthetic biology"],"downloads":0,"html":""},"search_terms":["translation","inhibition","resource","balance","cell","free","gene","expression","system","nagaraj","greene","sengupta","","sontag"],"keywords":["cell-free systems","in vitro synthetic biology"],"authorIDs":["5bc814f9db768e100000015a"],"dataSources":["DKqZbTmd7peqE4THw"]}