@article{Nash2010-pv,
	abstract = {A new diagnostic system for the enrichment and detection of
              protein biomarkers from human plasma is presented. Gold
              nanoparticles (AuNPs) were surface-modified with a diblock
              copolymer synthesized using reversible addition-fragmentation
              chain transfer (RAFT) polymerization. The diblock copolymer
              contained a thermally responsive poly(N-isopropylacrylamide)
              (pNIPAAm) block, a cationic amine-containing block, and a
              semi-telechelic PEG₂-biotin end group. When a mixed suspension of
              23 nm pNIPAAm-modified AuNPs was heated with pNIPAAm-coated 10 nm
              iron oxide magnetic nanoparticles (mNPs) in human plasma, the
              thermally responsive pNIPAAm directed the formation of mixed
              AuNP/mNP aggregates that could be separated efficiently with a
              magnet. Model studies showed that this mixed nanoparticle system
              could efficiently purify and strongly enrich the model biomarker
              protein streptavidin in spiked human plasma. A 10 ng/mL
              streptavidin sample was mixed with the biotinylated
              pNIPAAm-modified AuNPs and magnetically separated in the mixed
              nanoparticle system with pNIPAAm mNPs. The aggregates were
              concentrated into a 50-fold smaller fluid volume at room
              temperature where the gold nanoparticle reagent redissolved with
              the streptavidin target still bound. The concentrated gold-labeled
              streptavidin could be subsequently analyzed directly using lateral
              flow immunochromatography. This rapid capture and enrichment
              module thus utilizes the mixed stimuli-responsive nanoparticle
              system to achieve concentration of a gold-labeled biomarker that
              can be directly analyzed using lateral flow or other rapid
              diagnostic strategies.},
	author = {Nash, Michael A and Yager, Paul and Hoffman, Allan S and Stayton, Patrick S},
	date-added = {2026-03-04 17:57:53 +0100},
	date-modified = {2026-03-04 17:57:53 +0100},
	journal = {Bioconjug. Chem.},
	language = {en},
	month = dec,
	number = 12,
	pages = {2197--2204},
	title = {Mixed stimuli-responsive magnetic and gold nanoparticle system for rapid purification, enrichment, and detection of biomarkers},
	volume = 21,
	year = 2010}

{"_id":"vYvmhGLE8LmJy2pjz","bibbaseid":"nash-yager-hoffman-stayton-mixedstimuliresponsivemagneticandgoldnanoparticlesystemforrapidpurificationenrichmentanddetectionofbiomarkers-2010","author_short":["Nash, M. A","Yager, P.","Hoffman, A. S","Stayton, P. S"],"bibdata":{"bibtype":"article","type":"article","abstract":"A new diagnostic system for the enrichment and detection of protein biomarkers from human plasma is presented. Gold nanoparticles (AuNPs) were surface-modified with a diblock copolymer synthesized using reversible addition-fragmentation chain transfer (RAFT) polymerization. The diblock copolymer contained a thermally responsive poly(N-isopropylacrylamide) (pNIPAAm) block, a cationic amine-containing block, and a semi-telechelic PEG₂-biotin end group. When a mixed suspension of 23 nm pNIPAAm-modified AuNPs was heated with pNIPAAm-coated 10 nm iron oxide magnetic nanoparticles (mNPs) in human plasma, the thermally responsive pNIPAAm directed the formation of mixed AuNP/mNP aggregates that could be separated efficiently with a magnet. Model studies showed that this mixed nanoparticle system could efficiently purify and strongly enrich the model biomarker protein streptavidin in spiked human plasma. A 10 ng/mL streptavidin sample was mixed with the biotinylated pNIPAAm-modified AuNPs and magnetically separated in the mixed nanoparticle system with pNIPAAm mNPs. The aggregates were concentrated into a 50-fold smaller fluid volume at room temperature where the gold nanoparticle reagent redissolved with the streptavidin target still bound. The concentrated gold-labeled streptavidin could be subsequently analyzed directly using lateral flow immunochromatography. This rapid capture and enrichment module thus utilizes the mixed stimuli-responsive nanoparticle system to achieve concentration of a gold-labeled biomarker that can be directly analyzed using lateral flow or other rapid diagnostic strategies.","author":[{"propositions":[],"lastnames":["Nash"],"firstnames":["Michael","A"],"suffixes":[]},{"propositions":[],"lastnames":["Yager"],"firstnames":["Paul"],"suffixes":[]},{"propositions":[],"lastnames":["Hoffman"],"firstnames":["Allan","S"],"suffixes":[]},{"propositions":[],"lastnames":["Stayton"],"firstnames":["Patrick","S"],"suffixes":[]}],"date-added":"2026-03-04 17:57:53 +0100","date-modified":"2026-03-04 17:57:53 +0100","journal":"Bioconjug. Chem.","language":"en","month":"December","number":"12","pages":"2197–2204","title":"Mixed stimuli-responsive magnetic and gold nanoparticle system for rapid purification, enrichment, and detection of biomarkers","volume":"21","year":"2010","bibtex":"@article{Nash2010-pv,\n\tabstract = {A new diagnostic system for the enrichment and detection of\n protein biomarkers from human plasma is presented. Gold\n nanoparticles (AuNPs) were surface-modified with a diblock\n copolymer synthesized using reversible addition-fragmentation\n chain transfer (RAFT) polymerization. The diblock copolymer\n contained a thermally responsive poly(N-isopropylacrylamide)\n (pNIPAAm) block, a cationic amine-containing block, and a\n semi-telechelic PEG₂-biotin end group. When a mixed suspension of\n 23 nm pNIPAAm-modified AuNPs was heated with pNIPAAm-coated 10 nm\n iron oxide magnetic nanoparticles (mNPs) in human plasma, the\n thermally responsive pNIPAAm directed the formation of mixed\n AuNP/mNP aggregates that could be separated efficiently with a\n magnet. Model studies showed that this mixed nanoparticle system\n could efficiently purify and strongly enrich the model biomarker\n protein streptavidin in spiked human plasma. A 10 ng/mL\n streptavidin sample was mixed with the biotinylated\n pNIPAAm-modified AuNPs and magnetically separated in the mixed\n nanoparticle system with pNIPAAm mNPs. The aggregates were\n concentrated into a 50-fold smaller fluid volume at room\n temperature where the gold nanoparticle reagent redissolved with\n the streptavidin target still bound. The concentrated gold-labeled\n streptavidin could be subsequently analyzed directly using lateral\n flow immunochromatography. This rapid capture and enrichment\n module thus utilizes the mixed stimuli-responsive nanoparticle\n system to achieve concentration of a gold-labeled biomarker that\n can be directly analyzed using lateral flow or other rapid\n diagnostic strategies.},\n\tauthor = {Nash, Michael A and Yager, Paul and Hoffman, Allan S and Stayton, Patrick S},\n\tdate-added = {2026-03-04 17:57:53 +0100},\n\tdate-modified = {2026-03-04 17:57:53 +0100},\n\tjournal = {Bioconjug. Chem.},\n\tlanguage = {en},\n\tmonth = dec,\n\tnumber = 12,\n\tpages = {2197--2204},\n\ttitle = {Mixed stimuli-responsive magnetic and gold nanoparticle system for rapid purification, enrichment, and detection of biomarkers},\n\tvolume = 21,\n\tyear = 2010}\n\n","author_short":["Nash, M. A","Yager, P.","Hoffman, A. S","Stayton, P. S"],"key":"Nash2010-pv","id":"Nash2010-pv","bibbaseid":"nash-yager-hoffman-stayton-mixedstimuliresponsivemagneticandgoldnanoparticlesystemforrapidpurificationenrichmentanddetectionofbiomarkers-2010","role":"author","urls":{},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/f/67jgF7EnRSTkEvKwJ/Nash, Michael_BibBase.bib","dataSources":["zaKhN8TTHysCxBGoB","LHZJjLhDCo2CQAhuq","p9KnZehb2w3gt9uiA","xcC6dkB6kQNAd76MK"],"keywords":[],"search_terms":["mixed","stimuli","responsive","magnetic","gold","nanoparticle","system","rapid","purification","enrichment","detection","biomarkers","nash","yager","hoffman","stayton"],"title":"Mixed stimuli-responsive magnetic and gold nanoparticle system for rapid purification, enrichment, and detection of biomarkers","year":2010}