@article{
 title = {Efficient transformation of Marchantia polymorpha that is haploid and has very small genome DNA},
 type = {article},
 year = {1997},
 identifiers = {[object Object]},
 pages = {519-523},
 volume = {84},
 websites = {http://linkinghub.elsevier.com/retrieve/pii/S0922-338X(97)81904-6},
 id = {e9d86d8e-da05-3ed0-92ce-1a3d16945dce},
 created = {2011-05-20T17:26:02.000Z},
 file_attached = {true},
 profile_id = {b6c31fe8-61c6-3818-89a8-62873f3171f3},
 group_id = {7bdcaa0c-1528-351f-a09a-f8da52223946},
 last_modified = {2014-11-07T03:19:59.000Z},
 read = {false},
 starred = {false},
 authored = {false},
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 abstract = {The genomic DNA content of a cultured cell of Marchantia polymorpha HYA-2F was examined using a flow cytometer. It was estimated to be 0.32 pg (C), with a G+C content of 57.1%. The DNA content was less than that of Arabidopsis thaliana. The frequency of transformation by Agrobacterium tumefaciens using a binary vector plasmid pBI121 in the presence of acetosyringone was approximately 10%. GUS expression analysis and Southern blotting analysis of the genomic DNA of transformants revealed that all regions of T-DNA on plasmid pBI121 were integrated into the genome of M. polymorpha.},
 bibtype = {article},
 author = {Nasu, M and Tani, K and Hattori, C and Honda, M and Shimaoka, T and Yamaguchi, N and Katoh, K},
 journal = {Journal of Fermentation and Bioengineering},
 number = {6}
}

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