Formation mechanism of human serum albumin monolayers on positively charged polymer microparticles. Nattich-Rak, M., Sadowska, M., Adamczyk, Z., Cieśla, M., & Ka̧kol, M. Colloids Surfaces B Biointerfaces, 159:929–936, nov, 2017. ![Formation mechanism of human serum albumin monolayers on positively charged polymer microparticles [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Human serum albumin (HSA) adsorption on positively and negatively charged polystyrene microparticles was studied at various pHs and NaCl concentrations. Thorough electrophoretic mobility measurements were carried out that enabled to monitor in situ the progress of protein adsorption. The maximum coverage of irreversibly adsorbed HSA on microparticles was determined by different concentration depletion methods, one of them involving AFM imaging of residual molecules. An anomalous adsorption of HSA on the positive microparticles was observed at pH 3.5 where the maximum coverage attained 1.0 mg m^\−2\ for NaCl concentrations of 0.05 M despite that the molecules were on average positively charged. For comparison, the maximum coverage of HSA on negatively charged microparticles was equal to 1.3 mg m^\−2\ at this pH and NaCl concentration. At pH 7.4 the maximum coverage on positive microparticles was equal to 2.1 mg m^\−2\ for 0.05 M NaCl concentration. On the other hand, for negative microparticles, negligible adsorption of HSA was observed at pH 7.4 and 9.7. These experimental data were adequately interpreted in terms of the random sequential adsorption approach exploiting the bead model of the HSA molecule. Different orientations of adsorbed molecules, inert alia, the edge-on orientation prevailing for positively charged microparticles at pH 7.4, were confirmed. This was explained in terms of a heterogeneous charge distribution over the HSA molecule prevailing for a wide range of pHs.
@article{Nattich-Rak2017,
abstract = {Human serum albumin (HSA) adsorption on positively and negatively charged polystyrene microparticles was studied at various pHs and NaCl concentrations. Thorough electrophoretic mobility measurements were carried out that enabled to monitor in situ the progress of protein adsorption. The maximum coverage of irreversibly adsorbed HSA on microparticles was determined by different concentration depletion methods, one of them involving AFM imaging of residual molecules. An anomalous adsorption of HSA on the positive microparticles was observed at pH 3.5 where the maximum coverage attained 1.0 mg m{\^{}}{\{}−2{\}} for NaCl concentrations of 0.05 M despite that the molecules were on average positively charged. For comparison, the maximum coverage of HSA on negatively charged microparticles was equal to 1.3 mg m{\^{}}{\{}−2{\}} at this pH and NaCl concentration. At pH 7.4 the maximum coverage on positive microparticles was equal to 2.1 mg m{\^{}}{\{}−2{\}} for 0.05 M NaCl concentration. On the other hand, for negative microparticles, negligible adsorption of HSA was observed at pH 7.4 and 9.7. These experimental data were adequately interpreted in terms of the random sequential adsorption approach exploiting the bead model of the HSA molecule. Different orientations of adsorbed molecules, inert alia, the edge-on orientation prevailing for positively charged microparticles at pH 7.4, were confirmed. This was explained in terms of a heterogeneous charge distribution over the HSA molecule prevailing for a wide range of pHs.},
author = {Nattich-Rak, Ma{\l}gorzata and Sadowska, Marta and Adamczyk, Zbigniew and Cie{\'{s}}la, Micha{\l} and K{\c{a}}kol, Ma{\l}gorzata},
doi = {10.1016/j.colsurfb.2017.08.051},
file = {:home/ciesla/OneDrive/artyku{\l}y/moje/47. Formation Mechanism of Human Serum Albumin Monolayers on Positively Charged Polymer Microparticles.pdf:pdf},
issn = {09277765},
journal = {Colloids Surfaces B Biointerfaces},
keywords = {Adsorption of HSA on microparticles,HSA adsorption on microparticles,HSA monolayers,Maximum coverage of HSA on microparticles,Monolayers of HSA on microparticles,Zeta potential of HSA covered microparticles},
month = {nov},
pages = {929--936},
title = {{Formation mechanism of human serum albumin monolayers on positively charged polymer microparticles}},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0927776517305684},
volume = {159},
year = {2017}
}
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The maximum coverage of irreversibly adsorbed HSA on microparticles was determined by different concentration depletion methods, one of them involving AFM imaging of residual molecules. An anomalous adsorption of HSA on the positive microparticles was observed at pH 3.5 where the maximum coverage attained 1.0 mg m^\\−2\\ for NaCl concentrations of 0.05 M despite that the molecules were on average positively charged. For comparison, the maximum coverage of HSA on negatively charged microparticles was equal to 1.3 mg m^\\−2\\ at this pH and NaCl concentration. At pH 7.4 the maximum coverage on positive microparticles was equal to 2.1 mg m^\\−2\\ for 0.05 M NaCl concentration. On the other hand, for negative microparticles, negligible adsorption of HSA was observed at pH 7.4 and 9.7. These experimental data were adequately interpreted in terms of the random sequential adsorption approach exploiting the bead model of the HSA molecule. 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