Transgenic elite indica rice plants expressing CryIAc delta- endotoxin of Bacillus thuringiensis are resistant against yellow stem borer (Scirpophaga incertulas). Nayak, P., Basu, D., Das, S., Basu, A., Ghosh, D., Ramakrishnan, N., A., Ghosh, M., & Sen, S., K. Proceedings of the National Academy of Sciences of the United States of America, 94(6):2111-2116, 1997. abstract bibtex Generation of insect-resistant, transgenic crop plants by expression of the insecticidal crystal protein (ICP) gene of Bacillus thuringiensis (Bt) is a standard crop improvement approach, In such cases, adequate expression of the most appropriate ICP against the target insect pest of the crop species is desirable. It is also considered advantageous to generate Bt-transgenics with multiple toxin systems to control rapid development of pest resistance to the ICP, Larvae of yellow stem borer (YSB), Scirpophaga incertulas, a major lepidopteran insect pest of rice, cause massive losses of rice yield, Studies on insect feeding and on the binding properties of ICP to brush border membrane receptors in the midgut of YSB larvae revealed that cryI4b and cryI4e are two individually suitable candidate genes for developing YSB-resistant rice, Programs were undertaken to develop Bt-transgenic rice with these ICP genes independently in a single cultivar, A cryIAe gene was reconstructed and placed under control of the maize ubiquitin 1 promoter, along with the first intron of the maize ubiquitin 1 gene, and the nos terminator, Tile gene construct was delivered to embryogenic calli of IR64, an elite indica rice cultivar, using the particle bombardment method Six highly expressive independent transgenic ICP lines were identified, Molecular analyses and insect-feeding assays of two such lines revealed that the transferred synthetic cryI4e gene was expressed stably in the T-2 generation of these lines and that the transgenic rice plants were highly toxic to YSB larvae and lessened the damage caused by their feeding.
@article{
title = {Transgenic elite indica rice plants expressing CryIAc delta- endotoxin of Bacillus thuringiensis are resistant against yellow stem borer (Scirpophaga incertulas)},
type = {article},
year = {1997},
pages = {2111-2116},
volume = {94},
websites = {<Go to ISI>://A1997WP33400007},
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abstract = {Generation of insect-resistant, transgenic crop plants by expression of the insecticidal crystal protein (ICP) gene of Bacillus thuringiensis (Bt) is a standard crop improvement approach, In such cases, adequate expression of the most appropriate ICP against the target insect pest of the crop species is desirable. It is also considered advantageous to generate Bt-transgenics with multiple toxin systems to control rapid development of pest resistance to the ICP, Larvae of yellow stem borer (YSB), Scirpophaga incertulas, a major lepidopteran insect pest of rice, cause massive losses of rice yield, Studies on insect feeding and on the binding properties of ICP to brush border membrane receptors in the midgut of YSB larvae revealed that cryI4b and cryI4e are two individually suitable candidate genes for developing YSB-resistant rice, Programs were undertaken to develop Bt-transgenic rice with these ICP genes independently in a single cultivar, A cryIAe gene was reconstructed and placed under control of the maize ubiquitin 1 promoter, along with the first intron of the maize ubiquitin 1 gene, and the nos terminator, Tile gene construct was delivered to embryogenic calli of IR64, an elite indica rice cultivar, using the particle bombardment method Six highly expressive independent transgenic ICP lines were identified, Molecular analyses and insect-feeding assays of two such lines revealed that the transferred synthetic cryI4e gene was expressed stably in the T-2 generation of these lines and that the transgenic rice plants were highly toxic to YSB larvae and lessened the damage caused by their feeding.},
bibtype = {article},
author = {Nayak, P and Basu, D and Das, S and Basu, A and Ghosh, D and Ramakrishnan, N A and Ghosh, M and Sen, S K},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = {6}
}
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