B lymphocytes undergo TLR2-dependent apoptosis upon Shigella infection. Nothelfer, K., Arena, E. T., Pinaud, L., Neunlist, M., Mozeleski, B., Belotserkovsky, I., Parsot, C., Dinadayala, P., Burger-Kentischer, A., Raqib, R., Sansonetti, P. J., & Phalipon, A. The Journal of Experimental Medicine, 211(6):1215--1229, June, 2014. doi abstract bibtex Antibody-mediated immunity to Shigella, the causative agent of bacillary dysentery, requires several episodes of infection to get primed and is short-lasting, suggesting that the B cell response is functionally impaired. We show that upon ex vivo infection of human colonic tissue, invasive S. flexneri interacts with and occasionally invades B lymphocytes. The induction of a type three secretion apparatus (T3SA)-dependent B cell death is observed in the human CL-01 B cell line in vitro, as well as in mouse B lymphocytes in vivo. In addition to cell death occurring in Shigella-invaded CL-01 B lymphocytes, we provide evidence that the T3SA needle tip protein IpaD can induce cell death in noninvaded cells. IpaD binds to and induces B cell apoptosis via TLR2, a signaling receptor thus far considered to result in activation of B lymphocytes. The presence of bacterial co-signals is required to sensitize B cells to apoptosis and to up-regulate tlr2, thus enhancing IpaD binding. Apoptotic B lymphocytes in contact with Shigella-IpaD are detected in rectal biopsies of infected individuals. This study therefore adds direct B lymphocyte targeting to the diversity of mechanisms used by Shigella to dampen the host immune response.
@article{ nothelfer_b_2014,
title = {B lymphocytes undergo {TLR}2-dependent apoptosis upon {Shigella} infection},
volume = {211},
doi = {10.1084/jem.20130914},
abstract = {Antibody-mediated immunity to Shigella, the causative agent of bacillary dysentery, requires several episodes of infection to get primed and is short-lasting, suggesting that the B cell response is functionally impaired. We show that upon ex vivo infection of human colonic tissue, invasive S. flexneri interacts with and occasionally invades B lymphocytes. The induction of a type three secretion apparatus (T3SA)-dependent B cell death is observed in the human CL-01 B cell line in vitro, as well as in mouse B lymphocytes in vivo. In addition to cell death occurring in Shigella-invaded CL-01 B lymphocytes, we provide evidence that the T3SA needle tip protein IpaD can induce cell death in noninvaded cells. IpaD binds to and induces B cell apoptosis via TLR2, a signaling receptor thus far considered to result in activation of B lymphocytes. The presence of bacterial co-signals is required to sensitize B cells to apoptosis and to up-regulate tlr2, thus enhancing IpaD binding. Apoptotic B lymphocytes in contact with Shigella-IpaD are detected in rectal biopsies of infected individuals. This study therefore adds direct B lymphocyte targeting to the diversity of mechanisms used by Shigella to dampen the host immune response.},
language = {en},
number = {6},
journal = {The Journal of Experimental Medicine},
author = {Nothelfer, Katharina and Arena, Ellen T. and Pinaud, Laurie and Neunlist, Michel and Mozeleski, Brian and Belotserkovsky, Ilia and Parsot, Claude and Dinadayala, Premkumar and Burger-Kentischer, Anke and Raqib, Rubhana and Sansonetti, Philippe J. and Phalipon, Armelle},
month = {June},
year = {2014},
pages = {1215--1229}
}
Downloads: 0
{"_id":"MAg7wt57ijrsc8X36","authorIDs":[],"author_short":["Nothelfer, K.","Arena, E.<nbsp>T.","Pinaud, L.","Neunlist, M.","Mozeleski, B.","Belotserkovsky, I.","Parsot, C.","Dinadayala, P.","Burger-Kentischer, A.","Raqib, R.","Sansonetti, P.<nbsp>J.","Phalipon, A."],"bibbaseid":"nothelfer-arena-pinaud-neunlist-mozeleski-belotserkovsky-parsot-dinadayala-burgerkentischer-raqib-sansonetti-phalipon-blymphocytesundergotlr2dependentapoptosisuponshigellainfection-2014","bibdata":{"abstract":"Antibody-mediated immunity to Shigella, the causative agent of bacillary dysentery, requires several episodes of infection to get primed and is short-lasting, suggesting that the B cell response is functionally impaired. We show that upon ex vivo infection of human colonic tissue, invasive S. flexneri interacts with and occasionally invades B lymphocytes. The induction of a type three secretion apparatus (T3SA)-dependent B cell death is observed in the human CL-01 B cell line in vitro, as well as in mouse B lymphocytes in vivo. In addition to cell death occurring in Shigella-invaded CL-01 B lymphocytes, we provide evidence that the T3SA needle tip protein IpaD can induce cell death in noninvaded cells. IpaD binds to and induces B cell apoptosis via TLR2, a signaling receptor thus far considered to result in activation of B lymphocytes. The presence of bacterial co-signals is required to sensitize B cells to apoptosis and to up-regulate tlr2, thus enhancing IpaD binding. Apoptotic B lymphocytes in contact with Shigella-IpaD are detected in rectal biopsies of infected individuals. This study therefore adds direct B lymphocyte targeting to the diversity of mechanisms used by Shigella to dampen the host immune response.","author":["Nothelfer, Katharina","Arena, Ellen T.","Pinaud, Laurie","Neunlist, Michel","Mozeleski, Brian","Belotserkovsky, Ilia","Parsot, Claude","Dinadayala, Premkumar","Burger-Kentischer, Anke","Raqib, Rubhana","Sansonetti, Philippe J.","Phalipon, Armelle"],"author_short":["Nothelfer, K.","Arena, E.<nbsp>T.","Pinaud, L.","Neunlist, M.","Mozeleski, B.","Belotserkovsky, I.","Parsot, C.","Dinadayala, P.","Burger-Kentischer, A.","Raqib, R.","Sansonetti, P.<nbsp>J.","Phalipon, A."],"bibtex":"@article{ nothelfer_b_2014,\n title = {B lymphocytes undergo {TLR}2-dependent apoptosis upon {Shigella} infection},\n volume = {211},\n doi = {10.1084/jem.20130914},\n abstract = {Antibody-mediated immunity to Shigella, the causative agent of bacillary dysentery, requires several episodes of infection to get primed and is short-lasting, suggesting that the B cell response is functionally impaired. We show that upon ex vivo infection of human colonic tissue, invasive S. flexneri interacts with and occasionally invades B lymphocytes. The induction of a type three secretion apparatus (T3SA)-dependent B cell death is observed in the human CL-01 B cell line in vitro, as well as in mouse B lymphocytes in vivo. In addition to cell death occurring in Shigella-invaded CL-01 B lymphocytes, we provide evidence that the T3SA needle tip protein IpaD can induce cell death in noninvaded cells. IpaD binds to and induces B cell apoptosis via TLR2, a signaling receptor thus far considered to result in activation of B lymphocytes. The presence of bacterial co-signals is required to sensitize B cells to apoptosis and to up-regulate tlr2, thus enhancing IpaD binding. Apoptotic B lymphocytes in contact with Shigella-IpaD are detected in rectal biopsies of infected individuals. This study therefore adds direct B lymphocyte targeting to the diversity of mechanisms used by Shigella to dampen the host immune response.},\n language = {en},\n number = {6},\n journal = {The Journal of Experimental Medicine},\n author = {Nothelfer, Katharina and Arena, Ellen T. and Pinaud, Laurie and Neunlist, Michel and Mozeleski, Brian and Belotserkovsky, Ilia and Parsot, Claude and Dinadayala, Premkumar and Burger-Kentischer, Anke and Raqib, Rubhana and Sansonetti, Philippe J. and Phalipon, Armelle},\n month = {June},\n year = {2014},\n pages = {1215--1229}\n}","bibtype":"article","doi":"10.1084/jem.20130914","id":"nothelfer_b_2014","journal":"The Journal of Experimental Medicine","key":"nothelfer_b_2014","language":"en","month":"June","number":"6","pages":"1215--1229","title":"B lymphocytes undergo TLR2-dependent apoptosis upon Shigella infection","type":"article","volume":"211","year":"2014","bibbaseid":"nothelfer-arena-pinaud-neunlist-mozeleski-belotserkovsky-parsot-dinadayala-burgerkentischer-raqib-sansonetti-phalipon-blymphocytesundergotlr2dependentapoptosisuponshigellainfection-2014","role":"author","urls":{},"downloads":0},"bibtype":"article","biburl":"https://copy.com/hwYBEyalcoSXeycD","creationDate":"2015-03-20T15:16:03.532Z","downloads":0,"keywords":[],"search_terms":["lymphocytes","undergo","tlr2","dependent","apoptosis","upon","shigella","infection","nothelfer","arena","pinaud","neunlist","mozeleski","belotserkovsky","parsot","dinadayala","burger-kentischer","raqib","sansonetti","phalipon"],"title":"B lymphocytes undergo TLR2-dependent apoptosis upon Shigella infection","year":2014,"dataSources":["5MkK9khFbZvmsLDrT"]}