Spatiotemporal gene expression trajectories reveal developmental hierarchies of the human cortex. Nowakowski, T. J, Bhaduri, A., Pollen, A. A, Alvarado, B., Mostajo-Radji, M. A, Di Lullo, E., Haeussler, M., Sandoval-Espinosa, C., Liu, S. J., Velmeshev, D., Ounadjela, J. R., Shuga, J., Wang, X., Lim, D. A, West, J. A, Leyrat, A. A, Kent, W J., & Kriegstein, A. R Science, 358(6368):1318–1323, December, 2017. abstract bibtex Systematic analyses of spatiotemporal gene expression trajectories during organogenesis have been challenging because diverse cell types at different stages of maturation and differentiation coexist in the emerging tissues. We identified discrete cell types as well as temporally and spatially restricted trajectories of radial glia maturation and neurogenesis in developing human telencephalon. These lineage-specific trajectories reveal the expression of neurogenic transcription factors in early radial glia and enriched activation of mammalian target of rapamycin signaling in outer radial glia. Across cortical areas, modest transcriptional differences among radial glia cascade into robust typological distinctions among maturing neurons. Together, our results support a mixed model of topographical, typological, and temporal hierarchies governing cell-type diversity in the developing human telencephalon, including distinct excitatory lineages emerging in rostral and caudal cerebral cortex.
@ARTICLE{Nowakowski2017-et,
title = "Spatiotemporal gene expression trajectories reveal developmental
hierarchies of the human cortex",
author = "Nowakowski, Tomasz J and Bhaduri, Aparna and Pollen, Alex A and
Alvarado, Beatriz and Mostajo-Radji, Mohammed A and Di Lullo,
Elizabeth and Haeussler, Maximilian and Sandoval-Espinosa, Carmen
and Liu, Siyuan John and Velmeshev, Dmitry and Ounadjela, Johain
Ryad and Shuga, Joe and Wang, Xiaohui and Lim, Daniel A and West,
Jay A and Leyrat, Anne A and Kent, W James and Kriegstein, Arnold
R",
abstract = "Systematic analyses of spatiotemporal gene expression
trajectories during organogenesis have been challenging because
diverse cell types at different stages of maturation and
differentiation coexist in the emerging tissues. We identified
discrete cell types as well as temporally and spatially
restricted trajectories of radial glia maturation and
neurogenesis in developing human telencephalon. These
lineage-specific trajectories reveal the expression of neurogenic
transcription factors in early radial glia and enriched
activation of mammalian target of rapamycin signaling in outer
radial glia. Across cortical areas, modest transcriptional
differences among radial glia cascade into robust typological
distinctions among maturing neurons. Together, our results
support a mixed model of topographical, typological, and temporal
hierarchies governing cell-type diversity in the developing human
telencephalon, including distinct excitatory lineages emerging in
rostral and caudal cerebral cortex.",
journal = "Science",
volume = 358,
number = 6368,
pages = "1318--1323",
month = dec,
year = 2017,
language = "en"
}
Downloads: 0
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R"],"bibdata":{"bibtype":"article","type":"article","title":"Spatiotemporal gene expression trajectories reveal developmental hierarchies of the human cortex","author":[{"propositions":[],"lastnames":["Nowakowski"],"firstnames":["Tomasz","J"],"suffixes":[]},{"propositions":[],"lastnames":["Bhaduri"],"firstnames":["Aparna"],"suffixes":[]},{"propositions":[],"lastnames":["Pollen"],"firstnames":["Alex","A"],"suffixes":[]},{"propositions":[],"lastnames":["Alvarado"],"firstnames":["Beatriz"],"suffixes":[]},{"propositions":[],"lastnames":["Mostajo-Radji"],"firstnames":["Mohammed","A"],"suffixes":[]},{"propositions":[],"lastnames":["Di","Lullo"],"firstnames":["Elizabeth"],"suffixes":[]},{"propositions":[],"lastnames":["Haeussler"],"firstnames":["Maximilian"],"suffixes":[]},{"propositions":[],"lastnames":["Sandoval-Espinosa"],"firstnames":["Carmen"],"suffixes":[]},{"propositions":[],"lastnames":["Liu"],"firstnames":["Siyuan","John"],"suffixes":[]},{"propositions":[],"lastnames":["Velmeshev"],"firstnames":["Dmitry"],"suffixes":[]},{"propositions":[],"lastnames":["Ounadjela"],"firstnames":["Johain","Ryad"],"suffixes":[]},{"propositions":[],"lastnames":["Shuga"],"firstnames":["Joe"],"suffixes":[]},{"propositions":[],"lastnames":["Wang"],"firstnames":["Xiaohui"],"suffixes":[]},{"propositions":[],"lastnames":["Lim"],"firstnames":["Daniel","A"],"suffixes":[]},{"propositions":[],"lastnames":["West"],"firstnames":["Jay","A"],"suffixes":[]},{"propositions":[],"lastnames":["Leyrat"],"firstnames":["Anne","A"],"suffixes":[]},{"propositions":[],"lastnames":["Kent"],"firstnames":["W","James"],"suffixes":[]},{"propositions":[],"lastnames":["Kriegstein"],"firstnames":["Arnold","R"],"suffixes":[]}],"abstract":"Systematic analyses of spatiotemporal gene expression trajectories during organogenesis have been challenging because diverse cell types at different stages of maturation and differentiation coexist in the emerging tissues. We identified discrete cell types as well as temporally and spatially restricted trajectories of radial glia maturation and neurogenesis in developing human telencephalon. These lineage-specific trajectories reveal the expression of neurogenic transcription factors in early radial glia and enriched activation of mammalian target of rapamycin signaling in outer radial glia. Across cortical areas, modest transcriptional differences among radial glia cascade into robust typological distinctions among maturing neurons. Together, our results support a mixed model of topographical, typological, and temporal hierarchies governing cell-type diversity in the developing human telencephalon, including distinct excitatory lineages emerging in rostral and caudal cerebral cortex.","journal":"Science","volume":"358","number":"6368","pages":"1318–1323","month":"December","year":"2017","language":"en","bibtex":"@ARTICLE{Nowakowski2017-et,\n title = \"Spatiotemporal gene expression trajectories reveal developmental\n hierarchies of the human cortex\",\n author = \"Nowakowski, Tomasz J and Bhaduri, Aparna and Pollen, Alex A and\n Alvarado, Beatriz and Mostajo-Radji, Mohammed A and Di Lullo,\n Elizabeth and Haeussler, Maximilian and Sandoval-Espinosa, Carmen\n and Liu, Siyuan John and Velmeshev, Dmitry and Ounadjela, Johain\n Ryad and Shuga, Joe and Wang, Xiaohui and Lim, Daniel A and West,\n Jay A and Leyrat, Anne A and Kent, W James and Kriegstein, Arnold\n R\",\n abstract = \"Systematic analyses of spatiotemporal gene expression\n trajectories during organogenesis have been challenging because\n diverse cell types at different stages of maturation and\n differentiation coexist in the emerging tissues. We identified\n discrete cell types as well as temporally and spatially\n restricted trajectories of radial glia maturation and\n neurogenesis in developing human telencephalon. These\n lineage-specific trajectories reveal the expression of neurogenic\n transcription factors in early radial glia and enriched\n activation of mammalian target of rapamycin signaling in outer\n radial glia. Across cortical areas, modest transcriptional\n differences among radial glia cascade into robust typological\n distinctions among maturing neurons. Together, our results\n support a mixed model of topographical, typological, and temporal\n hierarchies governing cell-type diversity in the developing human\n telencephalon, including distinct excitatory lineages emerging in\n rostral and caudal cerebral cortex.\",\n journal = \"Science\",\n volume = 358,\n number = 6368,\n pages = \"1318--1323\",\n month = dec,\n year = 2017,\n language = \"en\"\n}\n\n","author_short":["Nowakowski, T. 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