@article{RN150,
   author = {O'Brien, T. J. and Passeri, M. and Lorenzo, M. F. and Sulzer, J. K. and Lyman, W. B. and Swet, J. H. and Vrochides, D. and Baker, E. H. and Iannitti, D. A. and Davalos, R. V. and McKillop, I. H.},
   title = {Experimental High-Frequency Irreversible Electroporation Using a Single-Needle Delivery Approach for Nonthermal Pancreatic Ablation In Vivo},
   journal = {J Vasc Interv Radiol},
   volume = {30},
   number = {6},
   pages = {854-862.e7},
   note = {1535-7732
O'Brien, Timothy J
Passeri, Michael
Lorenzo, Melvin F
Sulzer, Jesse K
Lyman, William B
Swet, Jacob H
Vrochides, Dionisios
Baker, Erin H
Iannitti, David A
Davalos, Rafael V
McKillop, Iain H
Journal Article
United States
2019/05/28
J Vasc Interv Radiol. 2019 Jun;30(6):854-862.e7. doi: 10.1016/j.jvir.2019.01.032.},
   abstract = {PURPOSE: To investigate the feasibility of single-needle high-frequency irreversible electroporation (SN-HFIRE) to create reproducible tissue ablations in an in vivo pancreatic swine model. MATERIALS AND METHODS: SN-HFIRE was performed in swine pancreas in vivo in the absence of intraoperative paralytics or cardiac synchronization using 3 different voltage waveforms (1-5-1, 2-5-2, and 5-5-5 [on-off-on times (μs)], n = 6/setting) with a total energized time of 100 μs per burst. At necropsy, ablation size/shape was determined. Immunohistochemistry was performed to quantify apoptosis using an anticleaved caspase-3 antibody. A numerical model was developed to determine lethal thresholds for each waveform in pancreas. RESULTS: Mean tissue ablation time was 5.0 ± 0.2 minutes, and no cardiac abnormalities or muscle twitch was detected. Mean ablation area significantly increased with increasing pulse width (41.0 ± 5.1 mm(2) [range 32-66 mm(2)] vs 44 ± 2.1 mm(2) [range 38-56 mm(2)] vs 85.0 ± 7.0 mm(2) [range 63-155 mm(2)]; 1-5-1, 2-5-2, 5-5-5, respectively; p < 0.0002 5-5-5 vs 1-5-1 and 2-5-2). The majority of the ablation zone did not stain positive for cleaved caspase-3 (6.1 ± 2.8% [range 1.8-9.1%], 8.8 ± 1.3% [range 5.5-14.0%], and 11.0 ± 1.4% [range 7.1-14.2%] cleaved caspase-3 positive 1-5-1, 2-5-2, 5-5-5, respectively), with significantly more positive staining at the 5-5-5 pulse setting compared with 1-5-1 (p < 0.03). Numerical modeling determined a lethal threshold of 1114 ± 123 V/cm (1-5-1 waveform), 1039 ± 103 V/cm (2-5-2 waveform), and 693 ± 81 V/cm (5-5-5 waveform). CONCLUSIONS: SN-HFIRE induces rapid, predictable ablations in pancreatic tissue in vivo without the need for intraoperative paralytics or cardiac synchronization.},
   keywords = {Ablation Techniques/*instrumentation/methods
Animals
Apoptosis
Caspase 3/metabolism
Electroporation/*instrumentation/methods
Feasibility Studies
Female
Finite Element Analysis
Models, Animal
Models, Theoretical
*Needles
Numerical Analysis, Computer-Assisted
Pancreas/metabolism/pathology/*surgery
Sus scrofa},
   ISSN = {1051-0443},
   DOI = {10.1016/j.jvir.2019.01.032},
   year = {2019},
   type = {Journal Article}
}

{"_id":"Xd6tkeRj8h6ug4car","bibbaseid":"obrien-passeri-lorenzo-sulzer-lyman-swet-vrochides-baker-etal-experimentalhighfrequencyirreversibleelectroporationusingasingleneedledeliveryapproachfornonthermalpancreaticablationinvivo-2019","author_short":["O'Brien, T. J.","Passeri, M.","Lorenzo, M. F.","Sulzer, J. K.","Lyman, W. B.","Swet, J. H.","Vrochides, D.","Baker, E. H.","Iannitti, D. A.","Davalos, R. V.","McKillop, I. H."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["O'Brien"],"firstnames":["T.","J."],"suffixes":[]},{"propositions":[],"lastnames":["Passeri"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Lorenzo"],"firstnames":["M.","F."],"suffixes":[]},{"propositions":[],"lastnames":["Sulzer"],"firstnames":["J.","K."],"suffixes":[]},{"propositions":[],"lastnames":["Lyman"],"firstnames":["W.","B."],"suffixes":[]},{"propositions":[],"lastnames":["Swet"],"firstnames":["J.","H."],"suffixes":[]},{"propositions":[],"lastnames":["Vrochides"],"firstnames":["D."],"suffixes":[]},{"propositions":[],"lastnames":["Baker"],"firstnames":["E.","H."],"suffixes":[]},{"propositions":[],"lastnames":["Iannitti"],"firstnames":["D.","A."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]},{"propositions":[],"lastnames":["McKillop"],"firstnames":["I.","H."],"suffixes":[]}],"title":"Experimental High-Frequency Irreversible Electroporation Using a Single-Needle Delivery Approach for Nonthermal Pancreatic Ablation In Vivo","journal":"J Vasc Interv Radiol","volume":"30","number":"6","pages":"854-862.e7","note":"1535-7732 O'Brien, Timothy J Passeri, Michael Lorenzo, Melvin F Sulzer, Jesse K Lyman, William B Swet, Jacob H Vrochides, Dionisios Baker, Erin H Iannitti, David A Davalos, Rafael V McKillop, Iain H Journal Article United States 2019/05/28 J Vasc Interv Radiol. 2019 Jun;30(6):854-862.e7. doi: 10.1016/j.jvir.2019.01.032.","abstract":"PURPOSE: To investigate the feasibility of single-needle high-frequency irreversible electroporation (SN-HFIRE) to create reproducible tissue ablations in an in vivo pancreatic swine model. MATERIALS AND METHODS: SN-HFIRE was performed in swine pancreas in vivo in the absence of intraoperative paralytics or cardiac synchronization using 3 different voltage waveforms (1-5-1, 2-5-2, and 5-5-5 [on-off-on times (μs)], n = 6/setting) with a total energized time of 100 μs per burst. At necropsy, ablation size/shape was determined. Immunohistochemistry was performed to quantify apoptosis using an anticleaved caspase-3 antibody. A numerical model was developed to determine lethal thresholds for each waveform in pancreas. RESULTS: Mean tissue ablation time was 5.0 ± 0.2 minutes, and no cardiac abnormalities or muscle twitch was detected. Mean ablation area significantly increased with increasing pulse width (41.0 ± 5.1 mm(2) [range 32-66 mm(2)] vs 44 ± 2.1 mm(2) [range 38-56 mm(2)] vs 85.0 ± 7.0 mm(2) [range 63-155 mm(2)]; 1-5-1, 2-5-2, 5-5-5, respectively; p < 0.0002 5-5-5 vs 1-5-1 and 2-5-2). The majority of the ablation zone did not stain positive for cleaved caspase-3 (6.1 ± 2.8% [range 1.8-9.1%], 8.8 ± 1.3% [range 5.5-14.0%], and 11.0 ± 1.4% [range 7.1-14.2%] cleaved caspase-3 positive 1-5-1, 2-5-2, 5-5-5, respectively), with significantly more positive staining at the 5-5-5 pulse setting compared with 1-5-1 (p < 0.03). Numerical modeling determined a lethal threshold of 1114 ± 123 V/cm (1-5-1 waveform), 1039 ± 103 V/cm (2-5-2 waveform), and 693 ± 81 V/cm (5-5-5 waveform). CONCLUSIONS: SN-HFIRE induces rapid, predictable ablations in pancreatic tissue in vivo without the need for intraoperative paralytics or cardiac synchronization.","keywords":"Ablation Techniques/*instrumentation/methods Animals Apoptosis Caspase 3/metabolism Electroporation/*instrumentation/methods Feasibility Studies Female Finite Element Analysis Models, Animal Models, Theoretical *Needles Numerical Analysis, Computer-Assisted Pancreas/metabolism/pathology/*surgery Sus scrofa","issn":"1051-0443","doi":"10.1016/j.jvir.2019.01.032","year":"2019","bibtex":"@article{RN150,\n author = {O'Brien, T. J. and Passeri, M. and Lorenzo, M. F. and Sulzer, J. K. and Lyman, W. B. and Swet, J. H. and Vrochides, D. and Baker, E. H. and Iannitti, D. A. and Davalos, R. V. and McKillop, I. H.},\n title = {Experimental High-Frequency Irreversible Electroporation Using a Single-Needle Delivery Approach for Nonthermal Pancreatic Ablation In Vivo},\n journal = {J Vasc Interv Radiol},\n volume = {30},\n number = {6},\n pages = {854-862.e7},\n note = {1535-7732\nO'Brien, Timothy J\nPasseri, Michael\nLorenzo, Melvin F\nSulzer, Jesse K\nLyman, William B\nSwet, Jacob H\nVrochides, Dionisios\nBaker, Erin H\nIannitti, David A\nDavalos, Rafael V\nMcKillop, Iain H\nJournal Article\nUnited States\n2019/05/28\nJ Vasc Interv Radiol. 2019 Jun;30(6):854-862.e7. doi: 10.1016/j.jvir.2019.01.032.},\n abstract = {PURPOSE: To investigate the feasibility of single-needle high-frequency irreversible electroporation (SN-HFIRE) to create reproducible tissue ablations in an in vivo pancreatic swine model. MATERIALS AND METHODS: SN-HFIRE was performed in swine pancreas in vivo in the absence of intraoperative paralytics or cardiac synchronization using 3 different voltage waveforms (1-5-1, 2-5-2, and 5-5-5 [on-off-on times (μs)], n = 6/setting) with a total energized time of 100 μs per burst. At necropsy, ablation size/shape was determined. Immunohistochemistry was performed to quantify apoptosis using an anticleaved caspase-3 antibody. A numerical model was developed to determine lethal thresholds for each waveform in pancreas. RESULTS: Mean tissue ablation time was 5.0 ± 0.2 minutes, and no cardiac abnormalities or muscle twitch was detected. Mean ablation area significantly increased with increasing pulse width (41.0 ± 5.1 mm(2) [range 32-66 mm(2)] vs 44 ± 2.1 mm(2) [range 38-56 mm(2)] vs 85.0 ± 7.0 mm(2) [range 63-155 mm(2)]; 1-5-1, 2-5-2, 5-5-5, respectively; p < 0.0002 5-5-5 vs 1-5-1 and 2-5-2). The majority of the ablation zone did not stain positive for cleaved caspase-3 (6.1 ± 2.8% [range 1.8-9.1%], 8.8 ± 1.3% [range 5.5-14.0%], and 11.0 ± 1.4% [range 7.1-14.2%] cleaved caspase-3 positive 1-5-1, 2-5-2, 5-5-5, respectively), with significantly more positive staining at the 5-5-5 pulse setting compared with 1-5-1 (p < 0.03). Numerical modeling determined a lethal threshold of 1114 ± 123 V/cm (1-5-1 waveform), 1039 ± 103 V/cm (2-5-2 waveform), and 693 ± 81 V/cm (5-5-5 waveform). CONCLUSIONS: SN-HFIRE induces rapid, predictable ablations in pancreatic tissue in vivo without the need for intraoperative paralytics or cardiac synchronization.},\n keywords = {Ablation Techniques/*instrumentation/methods\nAnimals\nApoptosis\nCaspase 3/metabolism\nElectroporation/*instrumentation/methods\nFeasibility Studies\nFemale\nFinite Element Analysis\nModels, Animal\nModels, Theoretical\n*Needles\nNumerical Analysis, Computer-Assisted\nPancreas/metabolism/pathology/*surgery\nSus scrofa},\n ISSN = {1051-0443},\n DOI = {10.1016/j.jvir.2019.01.032},\n year = {2019},\n type = {Journal Article}\n}\n\n","author_short":["O'Brien, T. J.","Passeri, M.","Lorenzo, M. F.","Sulzer, J. K.","Lyman, W. B.","Swet, J. H.","Vrochides, D.","Baker, E. H.","Iannitti, D. A.","Davalos, R. V.","McKillop, I. H."],"key":"RN150","id":"RN150","bibbaseid":"obrien-passeri-lorenzo-sulzer-lyman-swet-vrochides-baker-etal-experimentalhighfrequencyirreversibleelectroporationusingasingleneedledeliveryapproachfornonthermalpancreaticablationinvivo-2019","role":"author","urls":{},"keyword":["Ablation Techniques/*instrumentation/methods Animals Apoptosis Caspase 3/metabolism Electroporation/*instrumentation/methods Feasibility Studies Female Finite Element Analysis Models","Animal Models","Theoretical *Needles Numerical Analysis","Computer-Assisted Pancreas/metabolism/pathology/*surgery Sus scrofa"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","ZPLjameRikygaiM9B","3XfNmZkLe6o8CvECW","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["ablation techniques/*instrumentation/methods animals apoptosis caspase 3/metabolism electroporation/*instrumentation/methods feasibility studies female finite element analysis models","animal models","theoretical *needles numerical analysis","computer-assisted pancreas/metabolism/pathology/*surgery sus scrofa"],"search_terms":["experimental","high","frequency","irreversible","electroporation","using","single","needle","delivery","approach","nonthermal","pancreatic","ablation","vivo","o'brien","passeri","lorenzo","sulzer","lyman","swet","vrochides","baker","iannitti","davalos","mckillop"],"title":"Experimental High-Frequency Irreversible Electroporation Using a Single-Needle Delivery Approach for Nonthermal Pancreatic Ablation In Vivo","year":2019}