Functional Genomic Analysis of the AUXIN RESPONSE FACTOR Gene Family Members in Arabidopsis thaliana: Unique and Overlapping Functions of ARF7 and ARF19. Okushima, Y., Overvoorde, P. J., Arima, K., Alonso, J. M., Chan, A., Chang, C., Ecker, J. R., Hughes, B., Lui, A., Nguyen, D., Onodera, C., Quach, H., Smith, A., Yu, G., & Theologis, A. Plant Cell, 17(2):444--463, February, 2005.
Functional Genomic Analysis of the AUXIN RESPONSE FACTOR Gene Family Members in Arabidopsis thaliana: Unique and Overlapping Functions of ARF7 and ARF19 [link]Paper  doi  abstract   bibtex   
The AUXIN RESPONSE FACTOR (ARF) gene family products, together with the AUXIN/INDOLE-3-ACETIC ACID proteins, regulate auxin-mediated transcriptional activation/repression. The biological function(s) of most ARFs is poorly understood. Here, we report the identification and characterization of T-DNA insertion lines for 18 of the 23 ARF gene family members in Arabidopsis thaliana. Most of the lines fail to show an obvious growth phenotype except of the previously identified arf2/hss, arf3/ett, arf5/mp, and arf7/nph4 mutants, suggesting that there are functional redundancies among the ARF proteins. Subsequently, we generated double mutants. arf7 arf19 has a strong auxin-related phenotype not observed in the arf7 and arf19 single mutants, including severely impaired lateral root formation and abnormal gravitropism in both hypocotyl and root. Global gene expression analysis revealed that auxin-induced gene expression is severely impaired in the arf7 single and arf7 arf19 double mutants. For example, the expression of several genes, such as those encoding members of LATERAL ORGAN BOUNDARIES domain proteins and AUXIN-REGULATED GENE INVOLVED IN ORGAN SIZE, are disrupted in the double mutant. The data suggest that the ARF7 and ARF19 proteins play essential roles in auxin-mediated plant development by regulating both unique and partially overlapping sets of target genes. These observations provide molecular insight into the unique and overlapping functions of ARF gene family members in Arabidopsis.
@article{ okushima_functional_2005,
  title = {Functional Genomic Analysis of the {AUXIN} {RESPONSE} {FACTOR} Gene Family Members in Arabidopsis thaliana: Unique and Overlapping Functions of {ARF}7 and {ARF}19},
  volume = {17},
  shorttitle = {Functional Genomic Analysis of the {AUXIN} {RESPONSE} {FACTOR} Gene Family Members in Arabidopsis thaliana},
  url = {http://www.plantcell.org/cgi/content/abstract/17/2/444},
  doi = {10.1105/tpc.104.028316},
  abstract = {The {AUXIN} {RESPONSE} {FACTOR} ({ARF}) gene family products, together with the {AUXIN}/{INDOLE}-3-{ACETIC} {ACID} proteins, regulate auxin-mediated transcriptional activation/repression. The biological function(s) of most {ARFs} is poorly understood. Here, we report the identification and characterization of T-{DNA} insertion lines for 18 of the 23 {ARF} gene family members in Arabidopsis thaliana. Most of the lines fail to show an obvious growth phenotype except of the previously identified arf2/hss, arf3/ett, arf5/mp, and arf7/nph4 mutants, suggesting that there are functional redundancies among the {ARF} proteins. Subsequently, we generated double mutants. arf7 arf19 has a strong auxin-related phenotype not observed in the arf7 and arf19 single mutants, including severely impaired lateral root formation and abnormal gravitropism in both hypocotyl and root. Global gene expression analysis revealed that auxin-induced gene expression is severely impaired in the arf7 single and arf7 arf19 double mutants. For example, the expression of several genes, such as those encoding members of {LATERAL} {ORGAN} {BOUNDARIES} domain proteins and {AUXIN}-{REGULATED} {GENE} {INVOLVED} {IN} {ORGAN} {SIZE}, are disrupted in the double mutant. The data suggest that the {ARF}7 and {ARF}19 proteins play essential roles in auxin-mediated plant development by regulating both unique and partially overlapping sets of target genes. These observations provide molecular insight into the unique and overlapping functions of {ARF} gene family members in Arabidopsis.},
  number = {2},
  urldate = {2011-01-19},
  journal = {Plant Cell},
  author = {Okushima, Yoko and Overvoorde, Paul J. and Arima, Kazunari and Alonso, Jose M. and Chan, April and Chang, Charlie and Ecker, Joseph R. and Hughes, Beth and Lui, Amy and Nguyen, Diana and Onodera, Courtney and Quach, Hong and Smith, Alison and Yu, Guixia and Theologis, Athanasios},
  month = {February},
  year = {2005},
  pages = {444--463}
}
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