@article{
 title = {Delivery of minimally dispersed liquid interfaces for sequential surface chemistry},
 type = {article},
 year = {2016},
 pages = {3015-3023},
 volume = {16},
 websites = {https://pubs.rsc.org/en/content/articlelanding/2016/lc/c6lc00473c#!divAbstract%0A},
 publisher = {Royal Society of Chemistry},
 id = {4b03b9fe-dc9a-3425-9f83-8b139d3b77a4},
 created = {2019-01-20T06:08:16.520Z},
 file_attached = {false},
 profile_id = {dc1fdcdf-637d-32ee-a353-6a1d76918405},
 last_modified = {2019-01-27T02:16:12.681Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {true},
 hidden = {false},
 citation_key = {ostromohov2016delivery},
 source_type = {article},
 private_publication = {false},
 abstract = {We present a method for sequential delivery of reagents to a reaction site with minimal dispersion of their interfaces. Using segmented flow to encapsulate the reagents as droplets, the dispersion between reagent plugs remains confined in a limited volume, while being transmitted to the reaction surface. In close prox-imity to the target surface, we use a passive array of microstructures for removal of the oil phase such that the original reagent sequence is reconstructed, and only the aqueous phase reaches the reaction surface. We provide a detailed analysis of the conditions under which the method can be applied and demonstrate maintaining a transition time of 560 ms between reagents transported to a reaction site over a distance of 60 cm. We implemented the method using a vertical microfluidic probe on an open surface, allowing contact-free interaction with biological samples, and demonstrated two examples of assays implemented using the method: measurements of receptor–ligand reaction kinetics and of the fluorescence response of immobilized GFP to local variations in pH. We believe that the method can be useful for studying the dy-namic response of cells and proteins to various stimuli, as well as for highly automated multi-step assays.},
 bibtype = {article},
 author = {Ostromohov, N. and Bercovici, M. and Kaigala, G. V.},
 doi = {10.1039/c6lc00473c},
 journal = {Lab on a Chip},
 number = {16}
}

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