Excitatory GABA responses in embryonic and neonatal cortical slices demonstrated by gramicidin perforated-patch recordings and calcium imaging. Owens, D F, Boyce, L H, Davis, M B, & Kriegstein, A R J Neurosci, 16(20):6414–6423, October, 1996. abstract bibtex Gramicidin perforated-patch-clamp recordings in brain slices were used to obtain an accurate assessment of the developmental change in the GABAA receptor reversal potential (EGABAA) in embryonic and early postnatal rat neocortical cells including neuroepithelial precursor cells, cortical plate neurons, and postnatal neocortical neurons. Our results demonstrate that there is a progressive negative shift in EGABAA with the most positive values found in the youngest cortical precursor cells. At the early stages of neocortical development, EGABAA is determined by the chloride (Cl-) gradient, and the internal chloride concentration ([Cl-]i) decreases with development. EGABAA is positive to the resting potential, indicating that GABA serves to depolarize developing neocortical cells. Consistent with this conclusion, GABAA receptor activation with muscimol was found-to increase the internal calcium concentration ([Ca2+]i) in both embryonic and early postnatal neocortical cells through the activation of voltage-gated calcium channels (VGCCs). Postnatal cells exhibit spontaneous postsynaptic synaptic currents, which are eliminated by bicuculline methiodide (BMI) but not glutamate receptor antagonists and reverse at the Cl- equilibrium potential. Likewise, brief spontaneous increases in [Ca2+]i, sensitive to BMI and TTX, are observed at the same ages, suggesting that endogenous synaptic GABAA receptor activation can depolarize cells and activate VGCCs. These results suggest that GABAA receptor-mediated depolarization may influence early neocortical developmental events, including neurogenesis and synaptogenesis, through the activation of Ca(2+)-dependent signal transduction pathways.
@ARTICLE{Owens1996-gh,
title = "Excitatory {GABA} responses in embryonic and neonatal cortical
slices demonstrated by gramicidin perforated-patch recordings and
calcium imaging",
author = "Owens, D F and Boyce, L H and Davis, M B and Kriegstein, A R",
abstract = "Gramicidin perforated-patch-clamp recordings in brain slices were
used to obtain an accurate assessment of the developmental change
in the GABAA receptor reversal potential (EGABAA) in embryonic
and early postnatal rat neocortical cells including
neuroepithelial precursor cells, cortical plate neurons, and
postnatal neocortical neurons. Our results demonstrate that there
is a progressive negative shift in EGABAA with the most positive
values found in the youngest cortical precursor cells. At the
early stages of neocortical development, EGABAA is determined by
the chloride (Cl-) gradient, and the internal chloride
concentration ([Cl-]i) decreases with development. EGABAA is
positive to the resting potential, indicating that GABA serves to
depolarize developing neocortical cells. Consistent with this
conclusion, GABAA receptor activation with muscimol was found-to
increase the internal calcium concentration ([Ca2+]i) in both
embryonic and early postnatal neocortical cells through the
activation of voltage-gated calcium channels (VGCCs). Postnatal
cells exhibit spontaneous postsynaptic synaptic currents, which
are eliminated by bicuculline methiodide (BMI) but not glutamate
receptor antagonists and reverse at the Cl- equilibrium
potential. Likewise, brief spontaneous increases in [Ca2+]i,
sensitive to BMI and TTX, are observed at the same ages,
suggesting that endogenous synaptic GABAA receptor activation can
depolarize cells and activate VGCCs. These results suggest that
GABAA receptor-mediated depolarization may influence early
neocortical developmental events, including neurogenesis and
synaptogenesis, through the activation of Ca(2+)-dependent signal
transduction pathways.",
journal = "J Neurosci",
volume = 16,
number = 20,
pages = "6414--6423",
month = oct,
year = 1996,
language = "en"
}
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{"_id":"vkNeEfWR9sfna6DTN","bibbaseid":"owens-boyce-davis-kriegstein-excitatorygabaresponsesinembryonicandneonatalcorticalslicesdemonstratedbygramicidinperforatedpatchrecordingsandcalciumimaging-1996","author_short":["Owens, D F","Boyce, L H","Davis, M B","Kriegstein, A R"],"bibdata":{"bibtype":"article","type":"article","title":"Excitatory GABA responses in embryonic and neonatal cortical slices demonstrated by gramicidin perforated-patch recordings and calcium imaging","author":[{"propositions":[],"lastnames":["Owens"],"firstnames":["D","F"],"suffixes":[]},{"propositions":[],"lastnames":["Boyce"],"firstnames":["L","H"],"suffixes":[]},{"propositions":[],"lastnames":["Davis"],"firstnames":["M","B"],"suffixes":[]},{"propositions":[],"lastnames":["Kriegstein"],"firstnames":["A","R"],"suffixes":[]}],"abstract":"Gramicidin perforated-patch-clamp recordings in brain slices were used to obtain an accurate assessment of the developmental change in the GABAA receptor reversal potential (EGABAA) in embryonic and early postnatal rat neocortical cells including neuroepithelial precursor cells, cortical plate neurons, and postnatal neocortical neurons. Our results demonstrate that there is a progressive negative shift in EGABAA with the most positive values found in the youngest cortical precursor cells. At the early stages of neocortical development, EGABAA is determined by the chloride (Cl-) gradient, and the internal chloride concentration ([Cl-]i) decreases with development. EGABAA is positive to the resting potential, indicating that GABA serves to depolarize developing neocortical cells. Consistent with this conclusion, GABAA receptor activation with muscimol was found-to increase the internal calcium concentration ([Ca2+]i) in both embryonic and early postnatal neocortical cells through the activation of voltage-gated calcium channels (VGCCs). Postnatal cells exhibit spontaneous postsynaptic synaptic currents, which are eliminated by bicuculline methiodide (BMI) but not glutamate receptor antagonists and reverse at the Cl- equilibrium potential. Likewise, brief spontaneous increases in [Ca2+]i, sensitive to BMI and TTX, are observed at the same ages, suggesting that endogenous synaptic GABAA receptor activation can depolarize cells and activate VGCCs. These results suggest that GABAA receptor-mediated depolarization may influence early neocortical developmental events, including neurogenesis and synaptogenesis, through the activation of Ca(2+)-dependent signal transduction pathways.","journal":"J Neurosci","volume":"16","number":"20","pages":"6414–6423","month":"October","year":"1996","language":"en","bibtex":"@ARTICLE{Owens1996-gh,\n title = \"Excitatory {GABA} responses in embryonic and neonatal cortical\n slices demonstrated by gramicidin perforated-patch recordings and\n calcium imaging\",\n author = \"Owens, D F and Boyce, L H and Davis, M B and Kriegstein, A R\",\n abstract = \"Gramicidin perforated-patch-clamp recordings in brain slices were\n used to obtain an accurate assessment of the developmental change\n in the GABAA receptor reversal potential (EGABAA) in embryonic\n and early postnatal rat neocortical cells including\n neuroepithelial precursor cells, cortical plate neurons, and\n postnatal neocortical neurons. Our results demonstrate that there\n is a progressive negative shift in EGABAA with the most positive\n values found in the youngest cortical precursor cells. At the\n early stages of neocortical development, EGABAA is determined by\n the chloride (Cl-) gradient, and the internal chloride\n concentration ([Cl-]i) decreases with development. EGABAA is\n positive to the resting potential, indicating that GABA serves to\n depolarize developing neocortical cells. Consistent with this\n conclusion, GABAA receptor activation with muscimol was found-to\n increase the internal calcium concentration ([Ca2+]i) in both\n embryonic and early postnatal neocortical cells through the\n activation of voltage-gated calcium channels (VGCCs). Postnatal\n cells exhibit spontaneous postsynaptic synaptic currents, which\n are eliminated by bicuculline methiodide (BMI) but not glutamate\n receptor antagonists and reverse at the Cl- equilibrium\n potential. Likewise, brief spontaneous increases in [Ca2+]i,\n sensitive to BMI and TTX, are observed at the same ages,\n suggesting that endogenous synaptic GABAA receptor activation can\n depolarize cells and activate VGCCs. These results suggest that\n GABAA receptor-mediated depolarization may influence early\n neocortical developmental events, including neurogenesis and\n synaptogenesis, through the activation of Ca(2+)-dependent signal\n transduction pathways.\",\n journal = \"J Neurosci\",\n volume = 16,\n number = 20,\n pages = \"6414--6423\",\n month = oct,\n year = 1996,\n language = \"en\"\n}\n\n","author_short":["Owens, D F","Boyce, L H","Davis, M B","Kriegstein, A R"],"key":"Owens1996-gh","id":"Owens1996-gh","bibbaseid":"owens-boyce-davis-kriegstein-excitatorygabaresponsesinembryonicandneonatalcorticalslicesdemonstratedbygramicidinperforatedpatchrecordingsandcalciumimaging-1996","role":"author","urls":{},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/f/EJMp3HRuxirjxpcXh/references.bib","dataSources":["sAFYeB74DpbdXM9NN","4zx9n2tbeLTix3Wxr","k3cdWrThyTh5o59Rm","hq9pebjzmsTuyxGGx","h8Atv2SAy4PmShg5j"],"keywords":[],"search_terms":["excitatory","gaba","responses","embryonic","neonatal","cortical","slices","demonstrated","gramicidin","perforated","patch","recordings","calcium","imaging","owens","boyce","davis","kriegstein"],"title":"Excitatory GABA responses in embryonic and neonatal cortical slices demonstrated by gramicidin perforated-patch recordings and calcium imaging","year":1996}