@ARTICLE{Owens1998-hc,
  title    = "Patterns of intracellular calcium fluctuation in precursor cells
              of the neocortical ventricular zone",
  author   = "Owens, D F and Kriegstein, A R",
  abstract = "Changes in intracellular free calcium concentration ([Ca2+]i) are
              known to influence a variety of events in developing neurons.
              Although spontaneous changes of [Ca2+]i have been examined in
              immature cortical neurons, the calcium dynamics of cortical
              precursor cells have received less attention. Using an intact
              cortical mantle and confocal laser microscopy, we examined the
              spatiotemporal patterns of spontaneous [Ca2+]i fluctuations in
              neocortical ventricular zone (VZ) cells in situ. The majority of
              activity consisted of single cells that displayed independent
              [Ca2+]i fluctuations. These events occurred in cells throughout
              the depth of the VZ. Immunohistochemical staining confirmed that
              these events occurred primarily in precursor cells rather than in
              postmitotic neurons. When imaging near the ventricular surface,
              synchronous spontaneous [Ca2+]i increases were frequently
              observed in pairs of adjacent cells. Cellular morphology,
              time-lapse imaging, and nuclear staining demonstrated that this
              activity occurred in mitotically active cells. A third and
              infrequently encountered pattern of activity consisted of
              coordinated spontaneous increases in [Ca2+]i in groups of
              neighboring VZ cells. The morphological characteristics of these
              cells and immunohistochemical staining suggested that the
              coordinated events occurred in gap junction-coupled precursor
              cells. All three patterns of activity were dependent on the
              release of Ca2+ from intracellular stores. These results
              demonstrate distinct patterns of spontaneous [Ca2+]i change in
              cortical precursor cells and raise the possibility that these
              dynamics may contribute to the regulation of neurogenesis.",
  journal  = "J Neurosci",
  volume   =  18,
  number   =  14,
  pages    = "5374--5388",
  month    =  jul,
  year     =  1998,
  language = "en"
}

{"_id":"zHnvKE256LRHRbffc","bibbaseid":"owens-kriegstein-patternsofintracellularcalciumfluctuationinprecursorcellsoftheneocorticalventricularzone-1998","author_short":["Owens, D F","Kriegstein, A R"],"bibdata":{"bibtype":"article","type":"article","title":"Patterns of intracellular calcium fluctuation in precursor cells of the neocortical ventricular zone","author":[{"propositions":[],"lastnames":["Owens"],"firstnames":["D","F"],"suffixes":[]},{"propositions":[],"lastnames":["Kriegstein"],"firstnames":["A","R"],"suffixes":[]}],"abstract":"Changes in intracellular free calcium concentration ([Ca2+]i) are known to influence a variety of events in developing neurons. Although spontaneous changes of [Ca2+]i have been examined in immature cortical neurons, the calcium dynamics of cortical precursor cells have received less attention. Using an intact cortical mantle and confocal laser microscopy, we examined the spatiotemporal patterns of spontaneous [Ca2+]i fluctuations in neocortical ventricular zone (VZ) cells in situ. The majority of activity consisted of single cells that displayed independent [Ca2+]i fluctuations. These events occurred in cells throughout the depth of the VZ. Immunohistochemical staining confirmed that these events occurred primarily in precursor cells rather than in postmitotic neurons. When imaging near the ventricular surface, synchronous spontaneous [Ca2+]i increases were frequently observed in pairs of adjacent cells. Cellular morphology, time-lapse imaging, and nuclear staining demonstrated that this activity occurred in mitotically active cells. A third and infrequently encountered pattern of activity consisted of coordinated spontaneous increases in [Ca2+]i in groups of neighboring VZ cells. The morphological characteristics of these cells and immunohistochemical staining suggested that the coordinated events occurred in gap junction-coupled precursor cells. All three patterns of activity were dependent on the release of Ca2+ from intracellular stores. These results demonstrate distinct patterns of spontaneous [Ca2+]i change in cortical precursor cells and raise the possibility that these dynamics may contribute to the regulation of neurogenesis.","journal":"J Neurosci","volume":"18","number":"14","pages":"5374–5388","month":"July","year":"1998","language":"en","bibtex":"@ARTICLE{Owens1998-hc,\n title = \"Patterns of intracellular calcium fluctuation in precursor cells\n of the neocortical ventricular zone\",\n author = \"Owens, D F and Kriegstein, A R\",\n abstract = \"Changes in intracellular free calcium concentration ([Ca2+]i) are\n known to influence a variety of events in developing neurons.\n Although spontaneous changes of [Ca2+]i have been examined in\n immature cortical neurons, the calcium dynamics of cortical\n precursor cells have received less attention. Using an intact\n cortical mantle and confocal laser microscopy, we examined the\n spatiotemporal patterns of spontaneous [Ca2+]i fluctuations in\n neocortical ventricular zone (VZ) cells in situ. The majority of\n activity consisted of single cells that displayed independent\n [Ca2+]i fluctuations. These events occurred in cells throughout\n the depth of the VZ. Immunohistochemical staining confirmed that\n these events occurred primarily in precursor cells rather than in\n postmitotic neurons. When imaging near the ventricular surface,\n synchronous spontaneous [Ca2+]i increases were frequently\n observed in pairs of adjacent cells. Cellular morphology,\n time-lapse imaging, and nuclear staining demonstrated that this\n activity occurred in mitotically active cells. A third and\n infrequently encountered pattern of activity consisted of\n coordinated spontaneous increases in [Ca2+]i in groups of\n neighboring VZ cells. The morphological characteristics of these\n cells and immunohistochemical staining suggested that the\n coordinated events occurred in gap junction-coupled precursor\n cells. All three patterns of activity were dependent on the\n release of Ca2+ from intracellular stores. These results\n demonstrate distinct patterns of spontaneous [Ca2+]i change in\n cortical precursor cells and raise the possibility that these\n dynamics may contribute to the regulation of neurogenesis.\",\n journal = \"J Neurosci\",\n volume = 18,\n number = 14,\n pages = \"5374--5388\",\n month = jul,\n year = 1998,\n language = \"en\"\n}\n\n","author_short":["Owens, D F","Kriegstein, A R"],"key":"Owens1998-hc","id":"Owens1998-hc","bibbaseid":"owens-kriegstein-patternsofintracellularcalciumfluctuationinprecursorcellsoftheneocorticalventricularzone-1998","role":"author","urls":{},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/f/EJMp3HRuxirjxpcXh/references.bib","dataSources":["sAFYeB74DpbdXM9NN","4zx9n2tbeLTix3Wxr","k3cdWrThyTh5o59Rm","hq9pebjzmsTuyxGGx","h8Atv2SAy4PmShg5j"],"keywords":[],"search_terms":["patterns","intracellular","calcium","fluctuation","precursor","cells","neocortical","ventricular","zone","owens","kriegstein"],"title":"Patterns of intracellular calcium fluctuation in precursor cells of the neocortical ventricular zone","year":1998}