Improving the nutritional value of Golden Rice through increased pro-vitamin A content. Paine, J., A., Shipton, C., A., Chaggar, S., Howells, R., M., Kennedy, M., J., Vernon, G., Wright, S., Y., Hinchliffe, E., Adams, J., L., Silverstone, A., L., & Drake, R. Nature Biotechnology, 23(4):482-487, Nature Publishing Group, 2005.
abstract   bibtex   
'Golden Rice' is a variety of rice engineered to produceß-carotene (pro-vitamin A) to help combat vitamin A deficiency, and it has been predicted that its contribution to alleviating vitamin A deficiency would be substantially improved through even higherß-carotene content. We hypothesized that the daffodil gene encoding phytoene synthase (psy), one of the two genes used to develop Golden Rice, was the limiting step inß-carotene accumulation. Through systematic testing of other plant psys, we identified a psy from maize that substantially increased carotenoid accumulation in a model plant system. We went on to develop 'Golden Rice 2' introducing this psy in combination with the Erwinia uredovora carotene desaturase (crtI) used to generate the original Golden Rice. We observed an increase in total carotenoids of up to 23-fold (maximum 37µg/g) compared to the original Golden Rice and a preferential accumulation ofß-carotene. [ABSTRACT FROM AUTHOR] Copyright of Nature Biotechnology is the property of Nature Publishing Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
@article{
 title = {Improving the nutritional value of Golden Rice through increased pro-vitamin A content},
 type = {article},
 year = {2005},
 identifiers = {[object Object]},
 keywords = {CAROTENES,ERWINIA,GENES,NUTRITION -- Evaluation,RICE -- Varieties,VITAMIN A deficiency},
 pages = {482-487},
 volume = {23},
 websites = {http://www.lib.ncsu.edu/cgi-bin/proxy.pl?server=http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=16668992&site=ehost-live&scope=site},
 publisher = {Nature Publishing Group},
 id = {499be237-9484-3756-9c8a-b1e82c1a3ae3},
 created = {2012-01-05T13:08:30.000Z},
 file_attached = {false},
 profile_id = {1a467167-0a41-3583-a6a3-034c31031332},
 group_id = {0e532975-1a47-38a4-ace8-4fe5968bcd72},
 last_modified = {2012-01-05T13:14:49.000Z},
 tags = {golden rice,human and animal health,nutrition,nutritional enhancement},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 source_type = {Journal Article},
 abstract = {'Golden Rice' is a variety of rice engineered to produceß-carotene (pro-vitamin A) to help combat vitamin A deficiency, and it has been predicted that its contribution to alleviating vitamin A deficiency would be substantially improved through even higherß-carotene content. We hypothesized that the daffodil gene encoding phytoene synthase (psy), one of the two genes used to develop Golden Rice, was the limiting step inß-carotene accumulation. Through systematic testing of other plant psys, we identified a psy from maize that substantially increased carotenoid accumulation in a model plant system. We went on to develop 'Golden Rice 2' introducing this psy in combination with the Erwinia uredovora carotene desaturase (crtI) used to generate the original Golden Rice. We observed an increase in total carotenoids of up to 23-fold (maximum 37µg/g) compared to the original Golden Rice and a preferential accumulation ofß-carotene. [ABSTRACT FROM AUTHOR] Copyright of Nature Biotechnology is the property of Nature Publishing Group and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)},
 bibtype = {article},
 author = {Paine, Jacqueline A and Shipton, Catherine A and Chaggar, Sunandha and Howells, Rhian M and Kennedy, Mike J and Vernon, Gareth and Wright, Susan Y and Hinchliffe, Edward and Adams, Jessica L and Silverstone, Aron L and Drake, Rachel},
 journal = {Nature Biotechnology},
 number = {4}
}

Downloads: 0