Auxin-induced signaling protein nanoclustering contributes to cell polarity formation

Auxin-induced signaling protein nanoclustering contributes to cell polarity formation. Pan, X., Fang, L., Liu, J., Senay-Aras, B., Lin, W., Zheng, S., Zhang, T., Guo, J., Manor, U., Van Norman, J., Chen, W., & Yang, Z. Nature Communications, 11(1):3914, 2020.

Paper doi abstract bibtex

Cell polarity is fundamental to the development of both eukaryotes and prokaryotes, yet the mechanisms behind its formation are not well understood. Here we found that, phytohormone auxin-induced, sterol-dependent nanoclustering of cell surface transmembrane receptor kinase 1 (TMK1) is critical for the formation of polarized domains at the plasma membrane (PM) during the morphogenesis of cotyledon pavement cells (PC) in Arabidopsis. Auxin-induced TMK1 nanoclustering stabilizes flotillin1-associated ordered nanodomains, which in turn promote the nanoclustering of ROP6 GTPase that acts downstream of TMK1 to regulate cortical microtubule organization. In turn, cortical microtubules further stabilize TMK1- and flotillin1-containing nanoclusters at the PM. Hence, we propose a new paradigm for polarity formation: A diffusive signal triggers cell polarization by promoting cell surface receptor-mediated nanoclustering of signaling components and cytoskeleton-mediated positive feedback that reinforces these nanodomains into polarized domains.

@article{Pan2020,
  abstract = {Cell polarity is fundamental to the development of both eukaryotes and prokaryotes, yet the mechanisms behind its formation are not well understood. Here we found that, phytohormone auxin-induced, sterol-dependent nanoclustering of cell surface transmembrane receptor kinase 1 (TMK1) is critical for the formation of polarized domains at the plasma membrane (PM) during the morphogenesis of cotyledon pavement cells (PC) in Arabidopsis. Auxin-induced TMK1 nanoclustering stabilizes flotillin1-associated ordered nanodomains, which in turn promote the nanoclustering of ROP6 GTPase that acts downstream of TMK1 to regulate cortical microtubule organization. In turn, cortical microtubules further stabilize TMK1- and flotillin1-containing nanoclusters at the PM. Hence, we propose a new paradigm for polarity formation: A diffusive signal triggers cell polarization by promoting cell surface receptor-mediated nanoclustering of signaling components and cytoskeleton-mediated positive feedback that reinforces these nanodomains into polarized domains.},
  author = {Pan, Xue and Fang, Linjing and Liu, Jianfeng and Senay-Aras, Betul and Lin, Wenwei and Zheng, Shuan and Zhang, Tong and Guo, Jingzhe and Manor, Uri and {Van Norman}, Jaimie and Chen, Weitao and Yang, Zhenbiao},
  doi = {10.1038/s41467-020-17602-w},
  issn = {2041-1723},
  journal = {Nature Communications},
  number = {1},
  pages = {3914},
  title = {{Auxin-induced signaling protein nanoclustering contributes to cell polarity formation}},
  url = {https://doi.org/10.1038/s41467-020-17602-w},
  volume = {11},
  year = {2020}
}

Downloads: 0

{"_id":"ZyBjgcANiCHiESuou","bibbaseid":"pan-fang-liu-senayaras-lin-zheng-zhang-guo-etal-auxininducedsignalingproteinnanoclusteringcontributestocellpolarityformation-2020","author_short":["Pan, X.","Fang, L.","Liu, J.","Senay-Aras, B.","Lin, W.","Zheng, S.","Zhang, T.","Guo, J.","Manor, U.","Van Norman, J.","Chen, W.","Yang, Z."],"bibdata":{"bibtype":"article","type":"article","abstract":"Cell polarity is fundamental to the development of both eukaryotes and prokaryotes, yet the mechanisms behind its formation are not well understood. Here we found that, phytohormone auxin-induced, sterol-dependent nanoclustering of cell surface transmembrane receptor kinase 1 (TMK1) is critical for the formation of polarized domains at the plasma membrane (PM) during the morphogenesis of cotyledon pavement cells (PC) in Arabidopsis. Auxin-induced TMK1 nanoclustering stabilizes flotillin1-associated ordered nanodomains, which in turn promote the nanoclustering of ROP6 GTPase that acts downstream of TMK1 to regulate cortical microtubule organization. In turn, cortical microtubules further stabilize TMK1- and flotillin1-containing nanoclusters at the PM. Hence, we propose a new paradigm for polarity formation: A diffusive signal triggers cell polarization by promoting cell surface receptor-mediated nanoclustering of signaling components and cytoskeleton-mediated positive feedback that reinforces these nanodomains into polarized domains.","author":[{"propositions":[],"lastnames":["Pan"],"firstnames":["Xue"],"suffixes":[]},{"propositions":[],"lastnames":["Fang"],"firstnames":["Linjing"],"suffixes":[]},{"propositions":[],"lastnames":["Liu"],"firstnames":["Jianfeng"],"suffixes":[]},{"propositions":[],"lastnames":["Senay-Aras"],"firstnames":["Betul"],"suffixes":[]},{"propositions":[],"lastnames":["Lin"],"firstnames":["Wenwei"],"suffixes":[]},{"propositions":[],"lastnames":["Zheng"],"firstnames":["Shuan"],"suffixes":[]},{"propositions":[],"lastnames":["Zhang"],"firstnames":["Tong"],"suffixes":[]},{"propositions":[],"lastnames":["Guo"],"firstnames":["Jingzhe"],"suffixes":[]},{"propositions":[],"lastnames":["Manor"],"firstnames":["Uri"],"suffixes":[]},{"propositions":[],"lastnames":["Van Norman"],"firstnames":["Jaimie"],"suffixes":[]},{"propositions":[],"lastnames":["Chen"],"firstnames":["Weitao"],"suffixes":[]},{"propositions":[],"lastnames":["Yang"],"firstnames":["Zhenbiao"],"suffixes":[]}],"doi":"10.1038/s41467-020-17602-w","issn":"2041-1723","journal":"Nature Communications","number":"1","pages":"3914","title":"Auxin-induced signaling protein nanoclustering contributes to cell polarity formation","url":"https://doi.org/10.1038/s41467-020-17602-w","volume":"11","year":"2020","bibtex":"@article{Pan2020,\r\n abstract = {Cell polarity is fundamental to the development of both eukaryotes and prokaryotes, yet the mechanisms behind its formation are not well understood. Here we found that, phytohormone auxin-induced, sterol-dependent nanoclustering of cell surface transmembrane receptor kinase 1 (TMK1) is critical for the formation of polarized domains at the plasma membrane (PM) during the morphogenesis of cotyledon pavement cells (PC) in Arabidopsis. Auxin-induced TMK1 nanoclustering stabilizes flotillin1-associated ordered nanodomains, which in turn promote the nanoclustering of ROP6 GTPase that acts downstream of TMK1 to regulate cortical microtubule organization. In turn, cortical microtubules further stabilize TMK1- and flotillin1-containing nanoclusters at the PM. Hence, we propose a new paradigm for polarity formation: A diffusive signal triggers cell polarization by promoting cell surface receptor-mediated nanoclustering of signaling components and cytoskeleton-mediated positive feedback that reinforces these nanodomains into polarized domains.},\r\n author = {Pan, Xue and Fang, Linjing and Liu, Jianfeng and Senay-Aras, Betul and Lin, Wenwei and Zheng, Shuan and Zhang, Tong and Guo, Jingzhe and Manor, Uri and {Van Norman}, Jaimie and Chen, Weitao and Yang, Zhenbiao},\r\n doi = {10.1038/s41467-020-17602-w},\r\n issn = {2041-1723},\r\n journal = {Nature Communications},\r\n number = {1},\r\n pages = {3914},\r\n title = {{Auxin-induced signaling protein nanoclustering contributes to cell polarity formation}},\r\n url = {https://doi.org/10.1038/s41467-020-17602-w},\r\n volume = {11},\r\n year = {2020}\r\n}\r\n\r\n","author_short":["Pan, X.","Fang, L.","Liu, J.","Senay-Aras, B.","Lin, W.","Zheng, S.","Zhang, T.","Guo, J.","Manor, U.","Van Norman, J.","Chen, W.","Yang, Z."],"key":"Pan2020","id":"Pan2020","bibbaseid":"pan-fang-liu-senayaras-lin-zheng-zhang-guo-etal-auxininducedsignalingproteinnanoclusteringcontributestocellpolarityformation-2020","role":"author","urls":{"Paper":"https://doi.org/10.1038/s41467-020-17602-w"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://github.com/salkmanorlab/manor_publications/raw/main/manorlab_pubs.bib","dataSources":["edXsq84pNeYoA6wo6","dr3nCm3wyZkR7nFZf"],"keywords":[],"search_terms":["auxin","induced","signaling","protein","nanoclustering","contributes","cell","polarity","formation","pan","fang","liu","senay-aras","lin","zheng","zhang","guo","manor","van norman","chen","yang"],"title":"Auxin-induced signaling protein nanoclustering contributes to cell polarity formation","year":2020}