Rapid ribosomal translocation depends on the conserved 18-55 base pair in P-site transfer RNA. Pan, D., Kirillov, S., Zhang, C., Hou, Y., & Cooperman, B. Nature Structural and Molecular Biology, 13(4):354-359, 2006. cited By 38![Rapid ribosomal translocation depends on the conserved 18-55 base pair in P-site transfer RNA [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The L shape of tRNA is stabilized by the 'tertiary core' region, which contains base-pairing interactions between the D and T loops. Distortions of the L shape accompany tRNA movement across the ribosomal surface. Here, using single-turnover rapid kinetics assays, we determine the effects of mutations within the tertiary core of P site-bound tRNA fMet on three measures of the rate of translocation, the part of the elongation cycle involving the most extensive tRNA movement. Mutations in the strictly conserved G18·U55 base pair result in as much as an 80-fold decrease in the rate of translocation, demonstrating the importance of the 18-55 interaction for rapid translocation. This implicates the core region as a locus for functionally important dynamic interactions with the ribosome and leads to the proposal that translocation of ribosome-bound tRNAs may be sequential rather than concerted. © 2006 Nature Publishing Group.
@ARTICLE{Pan2006354,
author={Pan, D. and Kirillov, S. and Zhang, C.-M. and Hou, Y.-M. and Cooperman, B.S.},
title={Rapid ribosomal translocation depends on the conserved 18-55 base pair in P-site transfer RNA},
journal={Nature Structural and Molecular Biology},
year={2006},
volume={13},
number={4},
pages={354-359},
doi={10.1038/nsmb1074},
note={cited By 38},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-33745044746&doi=10.1038%2fnsmb1074&partnerID=40&md5=44d2957da3baa1f98417ed8d4997f51f},
affiliation={Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323, United States; Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107, United States; Petersburg Nuclear Physics Institute RAS, 188300 Gatchina, Russian Federation},
abstract={The L shape of tRNA is stabilized by the 'tertiary core' region, which contains base-pairing interactions between the D and T loops. Distortions of the L shape accompany tRNA movement across the ribosomal surface. Here, using single-turnover rapid kinetics assays, we determine the effects of mutations within the tertiary core of P site-bound tRNA fMet on three measures of the rate of translocation, the part of the elongation cycle involving the most extensive tRNA movement. Mutations in the strictly conserved G18·U55 base pair result in as much as an 80-fold decrease in the rate of translocation, demonstrating the importance of the 18-55 interaction for rapid translocation. This implicates the core region as a locus for functionally important dynamic interactions with the ribosome and leads to the proposal that translocation of ribosome-bound tRNAs may be sequential rather than concerted. © 2006 Nature Publishing Group.},
funding_details={GM56662, GM071014},
}
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