Multiplex-PCR for simultaneous detection of 3 bacterial fish pathogens, Flavobacterium columnare, Edwardsiella ictaluri, and Aeromonas hydrophila. Panangala, V. S., Shoemaker, C. A., Santen, V. L. v., Dybvig, K., & Klesius, P. H. Diseases of Aquatic Organisms, 74(3):199–208, March, 2007. ![Multiplex-PCR for simultaneous detection of 3 bacterial fish pathogens, Flavobacterium columnare, Edwardsiella ictaluri, and Aeromonas hydrophila [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex A multiplex PCR (m-PCR) method was developed for simultaneous detection of 3 important fish pathogens in warm water aquaculture. The m-PCR to amplify target DNA fragments from Flavobacterium columnare (504 bp), Edwardsiella ictaluri (407 bp) and Aeromonas hydrophila (209 bp) was optimized by adjustment of reaction buffers and a touchdown protocol. The lower detection limit for each of the 3 bacteria was 20 pg of nucleic acid template from each bacteria per m-PCR reaction mixture. The sensitivity threshold for detection of the 3 bacteria in tissues ranged between 3.4 × 102 and 2.5 × 105 cells g–1 of tissue (channel catfish Ictalurus punctatus Rafinesque). The diagnostic sensitivity and specificity of the m-PCR was evaluated with 10 representative isolates of each of the 3 bacteria and 11 other Gram-negative and 2 Gram-positive bacteria that are taxonomically related or ubiquitous in the aquatic environment. Except for a single species (A. salmonicida subsp. salmonicida), each set of primers specifically amplified the target DNA of the cognate species of bacteria. m-PCR was compared with bacteriological culture for identification of bacteria in experimentally infected fish. The m-PCR appears promising for the rapid, sensitive and simultaneous detection of Flavobacterium columnare, E. ictaluri and A. hydrophila in infected fish compared to the time-consuming traditional bacteriological culture techniques.
@article{panangala_multiplex-pcr_2007,
title = {Multiplex-{PCR} for simultaneous detection of 3 bacterial fish pathogens, {Flavobacterium} columnare, {Edwardsiella} ictaluri, and {Aeromonas} hydrophila},
volume = {74},
issn = {0177-5103, 1616-1580},
url = {https://www.int-res.com/abstracts/dao/v74/n3/p199-208/},
doi = {10.3354/dao074199},
abstract = {A multiplex PCR (m-PCR) method was developed for simultaneous detection of 3 important fish pathogens in warm water aquaculture. The m-PCR to amplify target DNA fragments from Flavobacterium columnare (504 bp), Edwardsiella ictaluri (407 bp) and Aeromonas hydrophila (209 bp) was optimized by adjustment of reaction buffers and a touchdown protocol. The lower detection limit for each of the 3 bacteria was 20 pg of nucleic acid template from each bacteria per m-PCR reaction mixture. The sensitivity threshold for detection of the 3 bacteria in tissues ranged between 3.4 × 102 and 2.5 × 105 cells g–1 of tissue (channel catfish Ictalurus punctatus Rafinesque). The diagnostic sensitivity and specificity of the m-PCR was evaluated with 10 representative isolates of each of the 3 bacteria and 11 other Gram-negative and 2 Gram-positive bacteria that are taxonomically related or ubiquitous in the aquatic environment. Except for a single species (A. salmonicida subsp. salmonicida), each set of primers specifically amplified the target DNA of the cognate species of bacteria. m-PCR was compared with bacteriological culture for identification of bacteria in experimentally infected fish. The m-PCR appears promising for the rapid, sensitive and simultaneous detection of Flavobacterium columnare, E. ictaluri and A. hydrophila in infected fish compared to the time-consuming traditional bacteriological culture techniques.},
language = {en},
number = {3},
urldate = {2024-05-27},
journal = {Diseases of Aquatic Organisms},
author = {Panangala, Victor S. and Shoemaker, Craig A. and Santen, Vicky L. van and Dybvig, Kevin and Klesius, Phillip H.},
month = mar,
year = {2007},
keywords = {Aeromonas, Edwardsiella, Fish, Flavobacterium, Multiplex-PCR},
pages = {199--208},
}
Downloads: 0
{"_id":"XNw9x8FwtayL7Yuqp","bibbaseid":"panangala-shoemaker-santen-dybvig-klesius-multiplexpcrforsimultaneousdetectionof3bacterialfishpathogensflavobacteriumcolumnareedwardsiellaictaluriandaeromonashydrophila-2007","author_short":["Panangala, V. S.","Shoemaker, C. A.","Santen, V. L. v.","Dybvig, K.","Klesius, P. H."],"bibdata":{"bibtype":"article","type":"article","title":"Multiplex-PCR for simultaneous detection of 3 bacterial fish pathogens, Flavobacterium columnare, Edwardsiella ictaluri, and Aeromonas hydrophila","volume":"74","issn":"0177-5103, 1616-1580","url":"https://www.int-res.com/abstracts/dao/v74/n3/p199-208/","doi":"10.3354/dao074199","abstract":"A multiplex PCR (m-PCR) method was developed for simultaneous detection of 3 important fish pathogens in warm water aquaculture. The m-PCR to amplify target DNA fragments from Flavobacterium columnare (504 bp), Edwardsiella ictaluri (407 bp) and Aeromonas hydrophila (209 bp) was optimized by adjustment of reaction buffers and a touchdown protocol. The lower detection limit for each of the 3 bacteria was 20 pg of nucleic acid template from each bacteria per m-PCR reaction mixture. The sensitivity threshold for detection of the 3 bacteria in tissues ranged between 3.4 × 102 and 2.5 × 105 cells g–1 of tissue (channel catfish Ictalurus punctatus Rafinesque). The diagnostic sensitivity and specificity of the m-PCR was evaluated with 10 representative isolates of each of the 3 bacteria and 11 other Gram-negative and 2 Gram-positive bacteria that are taxonomically related or ubiquitous in the aquatic environment. Except for a single species (A. salmonicida subsp. salmonicida), each set of primers specifically amplified the target DNA of the cognate species of bacteria. m-PCR was compared with bacteriological culture for identification of bacteria in experimentally infected fish. The m-PCR appears promising for the rapid, sensitive and simultaneous detection of Flavobacterium columnare, E. ictaluri and A. hydrophila in infected fish compared to the time-consuming traditional bacteriological culture techniques.","language":"en","number":"3","urldate":"2024-05-27","journal":"Diseases of Aquatic Organisms","author":[{"propositions":[],"lastnames":["Panangala"],"firstnames":["Victor","S."],"suffixes":[]},{"propositions":[],"lastnames":["Shoemaker"],"firstnames":["Craig","A."],"suffixes":[]},{"propositions":[],"lastnames":["Santen"],"firstnames":["Vicky","L.","van"],"suffixes":[]},{"propositions":[],"lastnames":["Dybvig"],"firstnames":["Kevin"],"suffixes":[]},{"propositions":[],"lastnames":["Klesius"],"firstnames":["Phillip","H."],"suffixes":[]}],"month":"March","year":"2007","keywords":"Aeromonas, Edwardsiella, Fish, Flavobacterium, Multiplex-PCR","pages":"199–208","bibtex":"@article{panangala_multiplex-pcr_2007,\n\ttitle = {Multiplex-{PCR} for simultaneous detection of 3 bacterial fish pathogens, {Flavobacterium} columnare, {Edwardsiella} ictaluri, and {Aeromonas} hydrophila},\n\tvolume = {74},\n\tissn = {0177-5103, 1616-1580},\n\turl = {https://www.int-res.com/abstracts/dao/v74/n3/p199-208/},\n\tdoi = {10.3354/dao074199},\n\tabstract = {A multiplex PCR (m-PCR) method was developed for simultaneous detection of 3 important fish pathogens in warm water aquaculture. The m-PCR to amplify target DNA fragments from Flavobacterium columnare (504 bp), Edwardsiella ictaluri (407 bp) and Aeromonas hydrophila (209 bp) was optimized by adjustment of reaction buffers and a touchdown protocol. The lower detection limit for each of the 3 bacteria was 20 pg of nucleic acid template from each bacteria per m-PCR reaction mixture. The sensitivity threshold for detection of the 3 bacteria in tissues ranged between 3.4 × 102 and 2.5 × 105 cells g–1 of tissue (channel catfish Ictalurus punctatus Rafinesque). The diagnostic sensitivity and specificity of the m-PCR was evaluated with 10 representative isolates of each of the 3 bacteria and 11 other Gram-negative and 2 Gram-positive bacteria that are taxonomically related or ubiquitous in the aquatic environment. Except for a single species (A. salmonicida subsp. salmonicida), each set of primers specifically amplified the target DNA of the cognate species of bacteria. m-PCR was compared with bacteriological culture for identification of bacteria in experimentally infected fish. The m-PCR appears promising for the rapid, sensitive and simultaneous detection of Flavobacterium columnare, E. ictaluri and A. hydrophila in infected fish compared to the time-consuming traditional bacteriological culture techniques.},\n\tlanguage = {en},\n\tnumber = {3},\n\turldate = {2024-05-27},\n\tjournal = {Diseases of Aquatic Organisms},\n\tauthor = {Panangala, Victor S. and Shoemaker, Craig A. and Santen, Vicky L. van and Dybvig, Kevin and Klesius, Phillip H.},\n\tmonth = mar,\n\tyear = {2007},\n\tkeywords = {Aeromonas, Edwardsiella, Fish, Flavobacterium, Multiplex-PCR},\n\tpages = {199--208},\n}\n\n","author_short":["Panangala, V. S.","Shoemaker, C. A.","Santen, V. L. v.","Dybvig, K.","Klesius, P. H."],"key":"panangala_multiplex-pcr_2007","id":"panangala_multiplex-pcr_2007","bibbaseid":"panangala-shoemaker-santen-dybvig-klesius-multiplexpcrforsimultaneousdetectionof3bacterialfishpathogensflavobacteriumcolumnareedwardsiellaictaluriandaeromonashydrophila-2007","role":"author","urls":{"Paper":"https://www.int-res.com/abstracts/dao/v74/n3/p199-208/"},"keyword":["Aeromonas","Edwardsiella","Fish","Flavobacterium","Multiplex-PCR"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://api.zotero.org/users/3734225/collections/D5KZ3DD8/items?key=Zsv3qpy77Y7ElTjxyhV5qury&format=bibtex&limit=100","dataSources":["HarKfoA9iZ96dtK7E"],"keywords":["aeromonas","edwardsiella","fish","flavobacterium","multiplex-pcr"],"search_terms":["multiplex","pcr","simultaneous","detection","bacterial","fish","pathogens","flavobacterium","columnare","edwardsiella","ictaluri","aeromonas","hydrophila","panangala","shoemaker","santen","dybvig","klesius"],"title":"Multiplex-PCR for simultaneous detection of 3 bacterial fish pathogens, Flavobacterium columnare, Edwardsiella ictaluri, and Aeromonas hydrophila","year":2007}