@article{RN282,
   author = {Paoli, L. and Ruscheweyh, H. J. and Forneris, C. C. and Hubrich, F. and Kautsar, S. and Bhushan, A. and Lotti, A. and Clayssen, Q. and Salazar, G. and Milanese, A. and Carlstrom, C. I. and Papadopoulou, C. and Gehrig, D. and Karasikov, M. and Mustafa, H. and Larralde, M. and Carroll, L. M. and Sanchez, P. and Zayed, A. A. and Cronin, D. R. and Acinas, S. G. and Bork, P. and Bowler, C. and Delmont, T. O. and Gasol, J. M. and Gossert, A. D. and Kahles, A. and Sullivan, M. B. and Wincker, P. and Zeller, G. and Robinson, S. L. and Piel, J. and Sunagawa, S.},
   title = {Biosynthetic potential of the global ocean microbiome},
   journal = {Nature},
   volume = {607},
   number = {7917},
   pages = {111-118},
   note = {Paoli, Lucas
Ruscheweyh, Hans-Joachim
Forneris, Clarissa C
Hubrich, Florian
Kautsar, Satria
Bhushan, Agneya
Lotti, Alessandro
Clayssen, Quentin
Salazar, Guillem
Milanese, Alessio
Carlstrom, Charlotte I
Papadopoulou, Chrysa
Gehrig, Daniel
Karasikov, Mikhail
Mustafa, Harun
Larralde, Martin
Carroll, Laura M
Sanchez, Pablo
Zayed, Ahmed A
Cronin, Dylan R
Acinas, Silvia G
Bork, Peer
Bowler, Chris
Delmont, Tom O
Gasol, Josep M
Gossert, Alvar D
Kahles, Andre
Sullivan, Matthew B
Wincker, Patrick
Zeller, Georg
Robinson, Serina L
Piel, Jorn
Sunagawa, Shinichi
eng
835067/ERC_/European Research Council/International
England
Nature. 2022 Jul;607(7917):111-118. doi: 10.1038/s41586-022-04862-3. Epub 2022 Jun 22.},
   abstract = {Natural microbial communities are phylogenetically and metabolically diverse. In addition to underexplored organismal groups(1), this diversity encompasses a rich discovery potential for ecologically and biotechnologically relevant enzymes and biochemical compounds(2,3). However, studying this diversity to identify genomic pathways for the synthesis of such compounds(4) and assigning them to their respective hosts remains challenging. The biosynthetic potential of microorganisms in the open ocean remains largely uncharted owing to limitations in the analysis of genome-resolved data at the global scale. Here we investigated the diversity and novelty of biosynthetic gene clusters in the ocean by integrating around 10,000 microbial genomes from cultivated and single cells with more than 25,000 newly reconstructed draft genomes from more than 1,000 seawater samples. These efforts revealed approximately 40,000 putative mostly new biosynthetic gene clusters, several of which were found in previously unsuspected phylogenetic groups. Among these groups, we identified a lineage rich in biosynthetic gene clusters ('Candidatus Eudoremicrobiaceae') that belongs to an uncultivated bacterial phylum and includes some of the most biosynthetically diverse microorganisms in this environment. From these, we characterized the phospeptin and pythonamide pathways, revealing cases of unusual bioactive compound structure and enzymology, respectively. Together, this research demonstrates how microbiomics-driven strategies can enable the investigation of previously undescribed enzymes and natural products in underexplored microbial groups and environments.},
   keywords = {Bacteria/classification/genetics
*Biosynthetic Pathways/genetics
Genomics
*Microbiota/genetics
Multigene Family/genetics
*Oceans and Seas
Phylogeny},
   ISSN = {1476-4687 (Electronic)
0028-0836 (Linking)},
   DOI = {10.1038/s41586-022-04862-3},
   url = {https://www.ncbi.nlm.nih.gov/pubmed/35732736},
   year = {2022},
   type = {Journal Article}
}

{"_id":"oDnxGxWCCyNLXnCXt","bibbaseid":"paoli-ruscheweyh-forneris-hubrich-kautsar-bhushan-lotti-clayssen-etal-biosyntheticpotentialoftheglobaloceanmicrobiome-2022","author_short":["Paoli, L.","Ruscheweyh, H. J.","Forneris, C. C.","Hubrich, F.","Kautsar, S.","Bhushan, A.","Lotti, A.","Clayssen, Q.","Salazar, G.","Milanese, A.","Carlstrom, C. I.","Papadopoulou, C.","Gehrig, D.","Karasikov, M.","Mustafa, H.","Larralde, M.","Carroll, L. M.","Sanchez, P.","Zayed, A. A.","Cronin, D. R.","Acinas, S. G.","Bork, P.","Bowler, C.","Delmont, T. O.","Gasol, J. M.","Gossert, A. D.","Kahles, A.","Sullivan, M. B.","Wincker, P.","Zeller, G.","Robinson, S. L.","Piel, J.","Sunagawa, S."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Paoli"],"firstnames":["L."],"suffixes":[]},{"propositions":[],"lastnames":["Ruscheweyh"],"firstnames":["H.","J."],"suffixes":[]},{"propositions":[],"lastnames":["Forneris"],"firstnames":["C.","C."],"suffixes":[]},{"propositions":[],"lastnames":["Hubrich"],"firstnames":["F."],"suffixes":[]},{"propositions":[],"lastnames":["Kautsar"],"firstnames":["S."],"suffixes":[]},{"propositions":[],"lastnames":["Bhushan"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Lotti"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Clayssen"],"firstnames":["Q."],"suffixes":[]},{"propositions":[],"lastnames":["Salazar"],"firstnames":["G."],"suffixes":[]},{"propositions":[],"lastnames":["Milanese"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Carlstrom"],"firstnames":["C.","I."],"suffixes":[]},{"propositions":[],"lastnames":["Papadopoulou"],"firstnames":["C."],"suffixes":[]},{"propositions":[],"lastnames":["Gehrig"],"firstnames":["D."],"suffixes":[]},{"propositions":[],"lastnames":["Karasikov"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Mustafa"],"firstnames":["H."],"suffixes":[]},{"propositions":[],"lastnames":["Larralde"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Carroll"],"firstnames":["L.","M."],"suffixes":[]},{"propositions":[],"lastnames":["Sanchez"],"firstnames":["P."],"suffixes":[]},{"propositions":[],"lastnames":["Zayed"],"firstnames":["A.","A."],"suffixes":[]},{"propositions":[],"lastnames":["Cronin"],"firstnames":["D.","R."],"suffixes":[]},{"propositions":[],"lastnames":["Acinas"],"firstnames":["S.","G."],"suffixes":[]},{"propositions":[],"lastnames":["Bork"],"firstnames":["P."],"suffixes":[]},{"propositions":[],"lastnames":["Bowler"],"firstnames":["C."],"suffixes":[]},{"propositions":[],"lastnames":["Delmont"],"firstnames":["T.","O."],"suffixes":[]},{"propositions":[],"lastnames":["Gasol"],"firstnames":["J.","M."],"suffixes":[]},{"propositions":[],"lastnames":["Gossert"],"firstnames":["A.","D."],"suffixes":[]},{"propositions":[],"lastnames":["Kahles"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Sullivan"],"firstnames":["M.","B."],"suffixes":[]},{"propositions":[],"lastnames":["Wincker"],"firstnames":["P."],"suffixes":[]},{"propositions":[],"lastnames":["Zeller"],"firstnames":["G."],"suffixes":[]},{"propositions":[],"lastnames":["Robinson"],"firstnames":["S.","L."],"suffixes":[]},{"propositions":[],"lastnames":["Piel"],"firstnames":["J."],"suffixes":[]},{"propositions":[],"lastnames":["Sunagawa"],"firstnames":["S."],"suffixes":[]}],"title":"Biosynthetic potential of the global ocean microbiome","journal":"Nature","volume":"607","number":"7917","pages":"111-118","note":"Paoli, Lucas Ruscheweyh, Hans-Joachim Forneris, Clarissa C Hubrich, Florian Kautsar, Satria Bhushan, Agneya Lotti, Alessandro Clayssen, Quentin Salazar, Guillem Milanese, Alessio Carlstrom, Charlotte I Papadopoulou, Chrysa Gehrig, Daniel Karasikov, Mikhail Mustafa, Harun Larralde, Martin Carroll, Laura M Sanchez, Pablo Zayed, Ahmed A Cronin, Dylan R Acinas, Silvia G Bork, Peer Bowler, Chris Delmont, Tom O Gasol, Josep M Gossert, Alvar D Kahles, Andre Sullivan, Matthew B Wincker, Patrick Zeller, Georg Robinson, Serina L Piel, Jorn Sunagawa, Shinichi eng 835067/ERC_/European Research Council/International England Nature. 2022 Jul;607(7917):111-118. doi: 10.1038/s41586-022-04862-3. Epub 2022 Jun 22.","abstract":"Natural microbial communities are phylogenetically and metabolically diverse. In addition to underexplored organismal groups(1), this diversity encompasses a rich discovery potential for ecologically and biotechnologically relevant enzymes and biochemical compounds(2,3). However, studying this diversity to identify genomic pathways for the synthesis of such compounds(4) and assigning them to their respective hosts remains challenging. The biosynthetic potential of microorganisms in the open ocean remains largely uncharted owing to limitations in the analysis of genome-resolved data at the global scale. Here we investigated the diversity and novelty of biosynthetic gene clusters in the ocean by integrating around 10,000 microbial genomes from cultivated and single cells with more than 25,000 newly reconstructed draft genomes from more than 1,000 seawater samples. These efforts revealed approximately 40,000 putative mostly new biosynthetic gene clusters, several of which were found in previously unsuspected phylogenetic groups. Among these groups, we identified a lineage rich in biosynthetic gene clusters ('Candidatus Eudoremicrobiaceae') that belongs to an uncultivated bacterial phylum and includes some of the most biosynthetically diverse microorganisms in this environment. From these, we characterized the phospeptin and pythonamide pathways, revealing cases of unusual bioactive compound structure and enzymology, respectively. 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M. and Gossert, A. D. and Kahles, A. and Sullivan, M. B. and Wincker, P. and Zeller, G. and Robinson, S. L. and Piel, J. and Sunagawa, S.},\r\n title = {Biosynthetic potential of the global ocean microbiome},\r\n journal = {Nature},\r\n volume = {607},\r\n number = {7917},\r\n pages = {111-118},\r\n note = {Paoli, Lucas\r\nRuscheweyh, Hans-Joachim\r\nForneris, Clarissa C\r\nHubrich, Florian\r\nKautsar, Satria\r\nBhushan, Agneya\r\nLotti, Alessandro\r\nClayssen, Quentin\r\nSalazar, Guillem\r\nMilanese, Alessio\r\nCarlstrom, Charlotte I\r\nPapadopoulou, Chrysa\r\nGehrig, Daniel\r\nKarasikov, Mikhail\r\nMustafa, Harun\r\nLarralde, Martin\r\nCarroll, Laura M\r\nSanchez, Pablo\r\nZayed, Ahmed A\r\nCronin, Dylan R\r\nAcinas, Silvia G\r\nBork, Peer\r\nBowler, Chris\r\nDelmont, Tom O\r\nGasol, Josep M\r\nGossert, Alvar D\r\nKahles, Andre\r\nSullivan, Matthew B\r\nWincker, Patrick\r\nZeller, Georg\r\nRobinson, Serina L\r\nPiel, Jorn\r\nSunagawa, Shinichi\r\neng\r\n835067/ERC_/European Research Council/International\r\nEngland\r\nNature. 2022 Jul;607(7917):111-118. doi: 10.1038/s41586-022-04862-3. 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These efforts revealed approximately 40,000 putative mostly new biosynthetic gene clusters, several of which were found in previously unsuspected phylogenetic groups. Among these groups, we identified a lineage rich in biosynthetic gene clusters ('Candidatus Eudoremicrobiaceae') that belongs to an uncultivated bacterial phylum and includes some of the most biosynthetically diverse microorganisms in this environment. From these, we characterized the phospeptin and pythonamide pathways, revealing cases of unusual bioactive compound structure and enzymology, respectively. 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