Isotachophoresis-Based Surface Immunoassay. Paratore, F., Zeidman Kalman, T., Rosenfeld, T., Kaigala, G., V., & Bercovici, M. Analytical Chemistry, 89(14):7373-7381, 7, 2017. ![Isotachophoresis-Based Surface Immunoassay [link]](https://bibbase.org/img/filetypes/link.svg "link")
Website doi abstract bibtex In the absence of amplification methods for proteins, the immune-detection of low-abundance proteins using antibodies is fundamentally limited by binding kinetic rates. Here, we present a new class of surface-based immunoassays in which protein–antibody reaction is accelerated by isotachophoresis (ITP). We demonstrate the use of ITP to preconcentrate and deliver target proteins to a surface decorated with specific antibodies, where effective utilization of the focused sample is achieved by modulating the driving electric field (stop-and-diffuse ITP mode) or applying a counter flow that opposes the ITP motion (counterflow ITP mode). Using enhanced green fluorescent protein (EGFP) as a model protein, we carry out an experimental optimization of the ITP-based immunoassay and demonstrate a 1300-fold improvement in limit of detection compared to a standard immunoassay, in a 6 min protein–antibody reaction. We discuss the design of buffer chemistries for other protein systems and, in concert with experiments, p...
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title = {Isotachophoresis-Based Surface Immunoassay},
type = {article},
year = {2017},
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abstract = {In the absence of amplification methods for proteins, the immune-detection of low-abundance proteins using antibodies is fundamentally limited by binding kinetic rates. Here, we present a new class of surface-based immunoassays in which protein–antibody reaction is accelerated by isotachophoresis (ITP). We demonstrate the use of ITP to preconcentrate and deliver target proteins to a surface decorated with specific antibodies, where effective utilization of the focused sample is achieved by modulating the driving electric field (stop-and-diffuse ITP mode) or applying a counter flow that opposes the ITP motion (counterflow ITP mode). Using enhanced green fluorescent protein (EGFP) as a model protein, we carry out an experimental optimization of the ITP-based immunoassay and demonstrate a 1300-fold improvement in limit of detection compared to a standard immunoassay, in a 6 min protein–antibody reaction. We discuss the design of buffer chemistries for other protein systems and, in concert with experiments, p...},
bibtype = {article},
author = {Paratore, Federico and Zeidman Kalman, Tal and Rosenfeld, Tally and Kaigala, Govind V. and Bercovici, Moran},
doi = {10.1021/acs.analchem.7b00725},
journal = {Analytical Chemistry},
number = {14}
}
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