Blood and gill responses to HCl infusions in the Pacific hagfish (Eptatretus stoutii). Parks, S. K., Tresguerres, M., & Goss, G. G. Canadian Journal of Zoology, 85(8):855–862, August, 2007. ![Blood and gill responses to HCl infusions in the Pacific hagfish (Eptatretus stoutii) [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Pacific hagfish (Eptatretus stoutii (Lockington, 1878)) were serially infused with HCl (6000 µmol·kg–1) every 6 h over a 24 h period to explore branchial acid secretion mechanisms. Blood pH recovered from a value ~2 pH units below that of resting pH. This pH regulatory mechanism was activated by 6 h and persisted over the 24 h time course. Western-blot analysis revealed an increased abundance (~2.3×) of NHE2-like immunoreactivity (NHE2 L-IR) in whole gill fractions from HCl-infused fish compared with control NaCl-infused fish. Membrane fractions isolated from the gills of HCl-infused fish also showed an increased abundance (~1.8×). Immunohistochemistry results demonstrated a significant redistribution of NHE2 L-IR to the apical region in HCl-infused fish. V-H+-ATPase abundance did not significantly increase in HCl-infused fish, while levels of Na+/K+-ATPase expression were unchanged. Immunohistochemistry of these transporters did not show any changes in cellular localization. We propose that the Pacific h..., Nous avons administré à des myxines brunes (Eptatretus stoutii (Lockington, 1878)) des séries d’injections d’HCl (6000 µmol·kg–1) à raison d’une injection à toutes les 6 h pendant une période de 24 h afin d’étudier les mécanismes de sécrétion acide des branchies. Le pH du sang peut se rétablir d’une valeur d’environ 2 unités de pH inférieure à la valeur de repos. Ce mécanisme régaluteur du pH est déjà en branle au bout de 6 h et persiste au cours des 24 h de l’expérience. Une analyse de buvardage Western indique une augmentation (~2,3×) de l’immunoréactivité de type NHE2 (NHE2 L-IR) dans les fractions de branchies entières chez les poissons infusés à l’HCl, par comparaison aux poissons témoins infusés au NaCl. Les fractions de membranes isolées des branchies de poissons infusés à l’HCl montrent aussi une augmentation (~1,8×). L’immunohistochimie révèle une redistribution significative de NHE2 L-IR vers la région apicale chez les poissons infusés à l’HCl. La V-H+-ATPase n’augmente pas significativement che...
@article{parks_blood_2007,
title = {Blood and gill responses to {HCl} infusions in the {Pacific} hagfish ({Eptatretus} stoutii)},
volume = {85},
issn = {0008-4301},
url = {http://www.nrcresearchpress.com/doi/abs/10.1139/Z07-068},
doi = {10.1139/Z07-068},
abstract = {Pacific hagfish (Eptatretus stoutii (Lockington, 1878)) were serially infused with HCl (6000 µmol·kg–1) every 6 h over a 24 h period to explore branchial acid secretion mechanisms. Blood pH recovered from a value {\textasciitilde}2 pH units below that of resting pH. This pH regulatory mechanism was activated by 6 h and persisted over the 24 h time course. Western-blot analysis revealed an increased abundance ({\textasciitilde}2.3×) of NHE2-like immunoreactivity (NHE2 L-IR) in whole gill fractions from HCl-infused fish compared with control NaCl-infused fish. Membrane fractions isolated from the gills of HCl-infused fish also showed an increased abundance ({\textasciitilde}1.8×). Immunohistochemistry results demonstrated a significant redistribution of NHE2 L-IR to the apical region in HCl-infused fish. V-H+-ATPase abundance did not significantly increase in HCl-infused fish, while levels of Na+/K+-ATPase expression were unchanged. Immunohistochemistry of these transporters did not show any changes in cellular localization. We propose that the Pacific h..., Nous avons administré à des myxines brunes (Eptatretus stoutii (Lockington, 1878)) des séries d’injections d’HCl (6000 µmol·kg–1) à raison d’une injection à toutes les 6 h pendant une période de 24 h afin d’étudier les mécanismes de sécrétion acide des branchies. Le pH du sang peut se rétablir d’une valeur d’environ 2 unités de pH inférieure à la valeur de repos. Ce mécanisme régaluteur du pH est déjà en branle au bout de 6 h et persiste au cours des 24 h de l’expérience. Une analyse de buvardage Western indique une augmentation ({\textasciitilde}2,3×) de l’immunoréactivité de type NHE2 (NHE2 L-IR) dans les fractions de branchies entières chez les poissons infusés à l’HCl, par comparaison aux poissons témoins infusés au NaCl. Les fractions de membranes isolées des branchies de poissons infusés à l’HCl montrent aussi une augmentation ({\textasciitilde}1,8×). L’immunohistochimie révèle une redistribution significative de NHE2 L-IR vers la région apicale chez les poissons infusés à l’HCl. La V-H+-ATPase n’augmente pas significativement che...},
number = {8},
urldate = {2015-08-24},
journal = {Canadian Journal of Zoology},
author = {Parks, Scott K. and Tresguerres, Martin and Goss, Greg G.},
month = aug,
year = {2007},
keywords = {Eptatretus stoutii},
pages = {855--862},
}
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This pH regulatory mechanism was activated by 6 h and persisted over the 24 h time course. Western-blot analysis revealed an increased abundance (~2.3×) of NHE2-like immunoreactivity (NHE2 L-IR) in whole gill fractions from HCl-infused fish compared with control NaCl-infused fish. Membrane fractions isolated from the gills of HCl-infused fish also showed an increased abundance (~1.8×). Immunohistochemistry results demonstrated a significant redistribution of NHE2 L-IR to the apical region in HCl-infused fish. V-H+-ATPase abundance did not significantly increase in HCl-infused fish, while levels of Na+/K+-ATPase expression were unchanged. Immunohistochemistry of these transporters did not show any changes in cellular localization. We propose that the Pacific h..., Nous avons administré à des myxines brunes (Eptatretus stoutii (Lockington, 1878)) des séries d’injections d’HCl (6000 µmol·kg–1) à raison d’une injection à toutes les 6 h pendant une période de 24 h afin d’étudier les mécanismes de sécrétion acide des branchies. Le pH du sang peut se rétablir d’une valeur d’environ 2 unités de pH inférieure à la valeur de repos. Ce mécanisme régaluteur du pH est déjà en branle au bout de 6 h et persiste au cours des 24 h de l’expérience. Une analyse de buvardage Western indique une augmentation (~2,3×) de l’immunoréactivité de type NHE2 (NHE2 L-IR) dans les fractions de branchies entières chez les poissons infusés à l’HCl, par comparaison aux poissons témoins infusés au NaCl. Les fractions de membranes isolées des branchies de poissons infusés à l’HCl montrent aussi une augmentation (~1,8×). 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