Downregulation of RWA genes in hybrid aspen affects xylan acetylation and wood saccharification. Pawar, P. M., Ratke, C., Balasubramanian, V. K., Chong, S., Gandla, M. L., Adriasola, M., Sparrman, T., Hedenström, M., Szwaj, K., Derba-Maceluch, M., Gaertner, C., Mouille, G., Ezcurra, I., Tenkanen, M., Jönsson, L. J., & Mellerowicz, E. J. New Phytologist, 214(4):1491–1505, 2017. _eprint: https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14489
Downregulation of RWA genes in hybrid aspen affects xylan acetylation and wood saccharification [link]Paper  doi  abstract   bibtex   
High acetylation of angiosperm wood hinders its conversion to sugars by glycoside hydrolases, subsequent ethanol fermentation and (hence) its use for biofuel production. We studied the REDUCED WALL ACETYLATION (RWA) gene family of the hardwood model Populus to evaluate its potential for improving saccharification. The family has two clades, AB and CD, containing two genes each. All four genes are expressed in developing wood but only RWA-A and -B are activated by master switches of the secondary cell wall PtNST1 and PtMYB21. Histochemical analysis of promoter::GUS lines in hybrid aspen (Populus tremula × tremuloides) showed activation of RWA-A and -B promoters in the secondary wall formation zone, while RWA-C and -D promoter activity was diffuse. Ectopic downregulation of either clade reduced wood xylan and xyloglucan acetylation. Suppressing both clades simultaneously using the wood-specific promoter reduced wood acetylation by 25% and decreased acetylation at position 2 of Xylp in the dimethyl sulfoxide-extracted xylan. This did not affect plant growth but decreased xylose and increased glucose contents in the noncellulosic monosaccharide fraction, and increased glucose and xylose yields of wood enzymatic hydrolysis without pretreatment. Both RWA clades regulate wood xylan acetylation in aspen and are promising targets to improve wood saccharification.
@article{pawar_downregulation_2017,
	title = {Downregulation of {RWA} genes in hybrid aspen affects xylan acetylation and wood saccharification},
	volume = {214},
	copyright = {© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust},
	issn = {1469-8137},
	url = {https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.14489},
	doi = {https://doi.org/10.1111/nph.14489},
	abstract = {High acetylation of angiosperm wood hinders its conversion to sugars by glycoside hydrolases, subsequent ethanol fermentation and (hence) its use for biofuel production. We studied the REDUCED WALL ACETYLATION (RWA) gene family of the hardwood model Populus to evaluate its potential for improving saccharification. The family has two clades, AB and CD, containing two genes each. All four genes are expressed in developing wood but only RWA-A and -B are activated by master switches of the secondary cell wall PtNST1 and PtMYB21. Histochemical analysis of promoter::GUS lines in hybrid aspen (Populus tremula × tremuloides) showed activation of RWA-A and -B promoters in the secondary wall formation zone, while RWA-C and -D promoter activity was diffuse. Ectopic downregulation of either clade reduced wood xylan and xyloglucan acetylation. Suppressing both clades simultaneously using the wood-specific promoter reduced wood acetylation by 25\% and decreased acetylation at position 2 of Xylp in the dimethyl sulfoxide-extracted xylan. This did not affect plant growth but decreased xylose and increased glucose contents in the noncellulosic monosaccharide fraction, and increased glucose and xylose yields of wood enzymatic hydrolysis without pretreatment. Both RWA clades regulate wood xylan acetylation in aspen and are promising targets to improve wood saccharification.},
	language = {en},
	number = {4},
	urldate = {2021-06-07},
	journal = {New Phytologist},
	author = {Pawar, Prashant Mohan-Anupama and Ratke, Christine and Balasubramanian, Vimal K. and Chong, Sun-Li and Gandla, Madhavi Latha and Adriasola, Mathilda and Sparrman, Tobias and Hedenström, Mattias and Szwaj, Klaudia and Derba-Maceluch, Marta and Gaertner, Cyril and Mouille, Gregory and Ezcurra, Ines and Tenkanen, Maija and Jönsson, Leif J. and Mellerowicz, Ewa J.},
	year = {2017},
	note = {\_eprint: https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14489},
	keywords = {Cas1p, Populus, REDUCED CELL WALL ACETYLATION, saccharification, wood acetylation, xylan, xylan acetylation, xylem},
	pages = {1491--1505},
}

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