Induction of parallel human telomeric G-quadruplex structures by Sr(2+). Pedroso, I. M, Duarte, L. F, Yanez, G., Baker, A. M, & Fletcher, T. M Biochemical and biophysical research communications, 358(1):298–303, July, 2007. ![Induction of parallel human telomeric G-quadruplex structures by Sr(2+). [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Human telomeric DNA forms G-quadruplex secondary structures, which can inhibit telomerase activity and are targets for anti-cancer drugs. Here we show that Sr(2+) can induce human telomeric DNA to form both inter- and intramolecular structures having characteristics consistent with G-quadruplexes. Unlike Na(+) or K(+), Sr(2+) facilitated intermolecular structure formation for oligonucleotides with 2 to 5 5'-d(TTAGGG)-3' repeats. Longer 5'-d(TTAGGG)-3' oligonucleotides formed exclusively intramolecular structures. Altering the 5'-d(TTAGGG)-3' to 5'-d(TTAGAG)-3' in the 1st, 3rd, or 4th repeats of 5'-d(TTAGGG)(4)-3' stabilized the formation of intermolecular structures. However, a more compact, intramolecular structure was still observed when the 2nd repeat was altered. Circular dichroism spectroscopy results suggest that the structures were parallel-stranded, distinguishing them from similar DNA sequences in Na(+) and K(+). This study shows that Sr(2+), promotes parallel-stranded, inter- and intramolecular G-quadruplexes that can serve as models to study DNA substrate recognition by telomerase.
@article{Pedroso2007,
title = {Induction of parallel human telomeric {G}-quadruplex structures by {Sr}(2+).},
volume = {358},
issn = {0006-291X},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17485073},
doi = {10.1016/j.bbrc.2007.04.126},
abstract = {Human telomeric DNA forms G-quadruplex secondary structures, which can inhibit telomerase activity and are targets for anti-cancer drugs. Here we show that Sr(2+) can induce human telomeric DNA to form both inter- and intramolecular structures having characteristics consistent with G-quadruplexes. Unlike Na(+) or K(+), Sr(2+) facilitated intermolecular structure formation for oligonucleotides with 2 to 5 5'-d(TTAGGG)-3' repeats. Longer 5'-d(TTAGGG)-3' oligonucleotides formed exclusively intramolecular structures. Altering the 5'-d(TTAGGG)-3' to 5'-d(TTAGAG)-3' in the 1st, 3rd, or 4th repeats of 5'-d(TTAGGG)(4)-3' stabilized the formation of intermolecular structures. However, a more compact, intramolecular structure was still observed when the 2nd repeat was altered. Circular dichroism spectroscopy results suggest that the structures were parallel-stranded, distinguishing them from similar DNA sequences in Na(+) and K(+). This study shows that Sr(2+), promotes parallel-stranded, inter- and intramolecular G-quadruplexes that can serve as models to study DNA substrate recognition by telomerase.},
number = {1},
journal = {Biochemical and biophysical research communications},
author = {Pedroso, Ilene M and Duarte, Luis F and Yanez, Giscard and Baker, Asmaa M and Fletcher, Terace M},
month = jul,
year = {2007},
pmid = {17485073},
keywords = {\#nosource, Cations, Circular Dichroism, DNA, DNA: chemistry, Divalent, Electrophoresis, Guanine, Guanine: chemistry, Humans, Nucleic Acid Conformation, Oligonucleotides, Oligonucleotides: chemistry, Polyacrylamide Gel, Potassium, Potassium: chemistry, Sodium, Sodium: chemistry, Strontium, Strontium: chemistry, Telomere, Telomere: chemistry},
pages = {298--303},
}
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