@article{
 title = {Mechanical and failure properties of single attached cells under compression.},
 type = {article},
 year = {2005},
 identifiers = {[object Object]},
 keywords = {Animals,Cell Adhesion,Cell Adhesion: physiology,Cell Line,Cell Membrane,Cell Membrane: physiology,Cell Membrane: ultrastructure,Cell Size,Compressive Strength,Compressive Strength: physiology,Computer Simulation,Elasticity,Membrane Fluidity,Membrane Fluidity: physiology,Mice,Models, Biological,Myoblasts, Skeletal,Myoblasts, Skeletal: cytology,Myoblasts, Skeletal: physiology,Physical Stimulation,Physical Stimulation: methods,Stress, Mechanical,Weight-Bearing,Weight-Bearing: physiology},
 pages = {1685-93},
 volume = {38},
 websites = {http://www.ncbi.nlm.nih.gov/pubmed/15958226},
 month = {8},
 id = {66c025fb-3dd3-3394-b400-cc36e9b68c29},
 created = {2012-12-06T09:13:40.000Z},
 accessed = {2010-10-31},
 file_attached = {true},
 profile_id = {0b777e31-8c9d-39dd-97a3-3e054bd99cfe},
 group_id = {764582e8-5773-3a66-8d6b-9b40e4fb5a88},
 last_modified = {2017-03-14T17:27:14.020Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 citation_key = {Peeters2005},
 abstract = {Eukaryotic cells are continuously subjected to mechanical forces under normal physiological conditions. These forces and associated cellular deformations induce a variety of biological processes. The degree of deformation depends on the mechanical properties of the cell. As most cells are anchorage dependent for normal functioning, it is important to study the mechanical properties of cells in their attached configuration. The goal of the present study was to obtain the mechanical and failure properties of attached cells. Individual, attached C2C12 mouse myoblasts were subjected to unconfined compression experiments using a recently developed loading device. The device allows global compression of the cell until cell rupture and simultaneously measures the associated forces. Cell bursting was characterized by a typical reduction in the force, referred to as the bursting force. Mean bursting forces were calculated as 8.7+/-2.5 microN at an axial strain of 72+/-4%. Visualization of the cell using confocal microscopy revealed that cell bursting was preceded by the formation of bulges at the cell membrane, which eventually led to rupturing of the cell membrane. Finite element calculations were performed to simulate the obtained force-deformation curves. A finite element mesh was built for each cell to account for its specific geometrical features. Using an axisymmetric approximation of the cell geometry, and a Neo-Hookean constitutive model, excellent agreement between predicted and measured force-deformation curves was obtained, yielding an average Young's modulus of 1.14+/-0.32 kPa.},
 bibtype = {article},
 author = {Peeters, E a G and Oomens, C W J and Bouten, C V C and Bader, D L and Baaijens, F P T},
 journal = {Journal of biomechanics},
 number = {8}
}

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These forces and associated cellular deformations induce a variety of biological processes. The degree of deformation depends on the mechanical properties of the cell. As most cells are anchorage dependent for normal functioning, it is important to study the mechanical properties of cells in their attached configuration. The goal of the present study was to obtain the mechanical and failure properties of attached cells. Individual, attached C2C12 mouse myoblasts were subjected to unconfined compression experiments using a recently developed loading device. The device allows global compression of the cell until cell rupture and simultaneously measures the associated forces. Cell bursting was characterized by a typical reduction in the force, referred to as the bursting force. Mean bursting forces were calculated as 8.7+/-2.5 microN at an axial strain of 72+/-4%. Visualization of the cell using confocal microscopy revealed that cell bursting was preceded by the formation of bulges at the cell membrane, which eventually led to rupturing of the cell membrane. Finite element calculations were performed to simulate the obtained force-deformation curves. A finite element mesh was built for each cell to account for its specific geometrical features. 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