@article{Peng2007,
	title = {G-quadruplex induced stabilization by 2'-deoxy-2'-fluoro-{D}-arabinonucleic acids (2'{F}-{ANA}).},
	volume = {35},
	issn = {1362-4962},
	url = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1976452&tool=pmcentrez&rendertype=abstract},
	doi = {10.1093/nar/gkm520},
	abstract = {The impact of 2'-deoxy-2'-fluoroarabinonucleotide residues (2'F-araN) on different G-quadruplexes derived from a thrombin-binding DNA aptamer d(G2T2G2TGTG2T2G2), an anti-HIV phosphorothioate aptamer PS-d(T2G4T2) and a DNA telomeric sequence d(G4T4G4) via UV thermal melting (T(m)) and circular dichroism (CD) experiments has been investigated. Generally, replacement of deoxyguanosines that adopt the anti conformation (anti-guanines) with 2'F-araG can stabilize G-quartets and maintain the quadruplex conformation, while replacement of syn-guanines with 2'F-araG is not favored and results in a dramatic switch to an alternative quadruplex conformation. It was found that incorporation of 2'F-araG or T residues into a thrombin-binding DNA G-quadruplex stabilizes the complex (DeltaT(m) up to approximately +3 degrees C/2'F-araN modification); 2'F-araN units also increased the half-life in 10\% fetal bovine serum (FBS) up to 48-fold. Two modified thrombin-binding aptamers (PG13 and PG14) show an approximately 4-fold increase in binding affinity to thrombin, as assessed via a nitrocellulose filter binding assay, both with increased thermal stability (approximately 1 degrees C/2'F-ANA modification increase in T(m)) and nuclease resistance (4-7-fold) as well. Therefore, the 2'-deoxy-2'-fluoro-d-arabinonucleic acid (2'F-ANA) modification is well suited to tune (and improve) the physicochemical and biological properties of naturally occurring DNA G-quartets.},
	number = {15},
	journal = {Nucleic acids research},
	author = {Peng, Chang Geng and Damha, Masad J},
	month = jan,
	year = {2007},
	pmid = {17636049},
	keywords = {\#nosource, Aptamers, Arabinonucleotides, Arabinonucleotides: chemistry, Circular Dichroism, DNA, DNA: chemistry, Deoxyribonucleases, Deoxyribonucleases: metabolism, G-Quadruplexes, Guanine, Guanine: chemistry, Models, Molecular, Nucleic Acid Conformation, Nucleotide, Nucleotide: chemistry, Nucleotide: metabolism, Telomere, Telomere: chemistry},
	pages = {4977--88},
}

{"_id":"PqtcE5Wm85pPy9Hjp","bibbaseid":"peng-damha-gquadruplexinducedstabilizationby2deoxy2fluorodarabinonucleicacids2fana-2007","author_short":["Peng, C. G.","Damha, M. J"],"bibdata":{"bibtype":"article","type":"article","title":"G-quadruplex induced stabilization by 2'-deoxy-2'-fluoro-D-arabinonucleic acids (2'F-ANA).","volume":"35","issn":"1362-4962","url":"http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1976452&tool=pmcentrez&rendertype=abstract","doi":"10.1093/nar/gkm520","abstract":"The impact of 2'-deoxy-2'-fluoroarabinonucleotide residues (2'F-araN) on different G-quadruplexes derived from a thrombin-binding DNA aptamer d(G2T2G2TGTG2T2G2), an anti-HIV phosphorothioate aptamer PS-d(T2G4T2) and a DNA telomeric sequence d(G4T4G4) via UV thermal melting (T(m)) and circular dichroism (CD) experiments has been investigated. Generally, replacement of deoxyguanosines that adopt the anti conformation (anti-guanines) with 2'F-araG can stabilize G-quartets and maintain the quadruplex conformation, while replacement of syn-guanines with 2'F-araG is not favored and results in a dramatic switch to an alternative quadruplex conformation. It was found that incorporation of 2'F-araG or T residues into a thrombin-binding DNA G-quadruplex stabilizes the complex (DeltaT(m) up to approximately +3 degrees C/2'F-araN modification); 2'F-araN units also increased the half-life in 10% fetal bovine serum (FBS) up to 48-fold. Two modified thrombin-binding aptamers (PG13 and PG14) show an approximately 4-fold increase in binding affinity to thrombin, as assessed via a nitrocellulose filter binding assay, both with increased thermal stability (approximately 1 degrees C/2'F-ANA modification increase in T(m)) and nuclease resistance (4-7-fold) as well. Therefore, the 2'-deoxy-2'-fluoro-d-arabinonucleic acid (2'F-ANA) modification is well suited to tune (and improve) the physicochemical and biological properties of naturally occurring DNA G-quartets.","number":"15","journal":"Nucleic acids research","author":[{"propositions":[],"lastnames":["Peng"],"firstnames":["Chang","Geng"],"suffixes":[]},{"propositions":[],"lastnames":["Damha"],"firstnames":["Masad","J"],"suffixes":[]}],"month":"January","year":"2007","pmid":"17636049","keywords":"#nosource, Aptamers, Arabinonucleotides, Arabinonucleotides: chemistry, Circular Dichroism, DNA, DNA: chemistry, Deoxyribonucleases, Deoxyribonucleases: metabolism, G-Quadruplexes, Guanine, Guanine: chemistry, Models, Molecular, Nucleic Acid Conformation, Nucleotide, Nucleotide: chemistry, Nucleotide: metabolism, Telomere, Telomere: chemistry","pages":"4977–88","bibtex":"@article{Peng2007,\n\ttitle = {G-quadruplex induced stabilization by 2'-deoxy-2'-fluoro-{D}-arabinonucleic acids (2'{F}-{ANA}).},\n\tvolume = {35},\n\tissn = {1362-4962},\n\turl = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1976452&tool=pmcentrez&rendertype=abstract},\n\tdoi = {10.1093/nar/gkm520},\n\tabstract = {The impact of 2'-deoxy-2'-fluoroarabinonucleotide residues (2'F-araN) on different G-quadruplexes derived from a thrombin-binding DNA aptamer d(G2T2G2TGTG2T2G2), an anti-HIV phosphorothioate aptamer PS-d(T2G4T2) and a DNA telomeric sequence d(G4T4G4) via UV thermal melting (T(m)) and circular dichroism (CD) experiments has been investigated. Generally, replacement of deoxyguanosines that adopt the anti conformation (anti-guanines) with 2'F-araG can stabilize G-quartets and maintain the quadruplex conformation, while replacement of syn-guanines with 2'F-araG is not favored and results in a dramatic switch to an alternative quadruplex conformation. It was found that incorporation of 2'F-araG or T residues into a thrombin-binding DNA G-quadruplex stabilizes the complex (DeltaT(m) up to approximately +3 degrees C/2'F-araN modification); 2'F-araN units also increased the half-life in 10\\% fetal bovine serum (FBS) up to 48-fold. Two modified thrombin-binding aptamers (PG13 and PG14) show an approximately 4-fold increase in binding affinity to thrombin, as assessed via a nitrocellulose filter binding assay, both with increased thermal stability (approximately 1 degrees C/2'F-ANA modification increase in T(m)) and nuclease resistance (4-7-fold) as well. Therefore, the 2'-deoxy-2'-fluoro-d-arabinonucleic acid (2'F-ANA) modification is well suited to tune (and improve) the physicochemical and biological properties of naturally occurring DNA G-quartets.},\n\tnumber = {15},\n\tjournal = {Nucleic acids research},\n\tauthor = {Peng, Chang Geng and Damha, Masad J},\n\tmonth = jan,\n\tyear = {2007},\n\tpmid = {17636049},\n\tkeywords = {\\#nosource, Aptamers, Arabinonucleotides, Arabinonucleotides: chemistry, Circular Dichroism, DNA, DNA: chemistry, Deoxyribonucleases, Deoxyribonucleases: metabolism, G-Quadruplexes, Guanine, Guanine: chemistry, Models, Molecular, Nucleic Acid Conformation, Nucleotide, Nucleotide: chemistry, Nucleotide: metabolism, Telomere, Telomere: chemistry},\n\tpages = {4977--88},\n}\n\n","author_short":["Peng, C. G.","Damha, M. J"],"key":"Peng2007","id":"Peng2007","bibbaseid":"peng-damha-gquadruplexinducedstabilizationby2deoxy2fluorodarabinonucleicacids2fana-2007","role":"author","urls":{"Paper":"http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1976452&tool=pmcentrez&rendertype=abstract"},"keyword":["#nosource","Aptamers","Arabinonucleotides","Arabinonucleotides: chemistry","Circular Dichroism","DNA","DNA: chemistry","Deoxyribonucleases","Deoxyribonucleases: metabolism","G-Quadruplexes","Guanine","Guanine: chemistry","Models","Molecular","Nucleic Acid Conformation","Nucleotide","Nucleotide: chemistry","Nucleotide: metabolism","Telomere","Telomere: chemistry"],"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/zotero/eric.larG4","dataSources":["4i5C7S78DvJNsaHyg","5L2zM5wNE5CBYNuea"],"keywords":["#nosource","aptamers","arabinonucleotides","arabinonucleotides: chemistry","circular dichroism","dna","dna: chemistry","deoxyribonucleases","deoxyribonucleases: metabolism","g-quadruplexes","guanine","guanine: chemistry","models","molecular","nucleic acid conformation","nucleotide","nucleotide: chemistry","nucleotide: metabolism","telomere","telomere: chemistry"],"search_terms":["quadruplex","induced","stabilization","deoxy","fluoro","arabinonucleic","acids","ana","peng","damha"],"title":"G-quadruplex induced stabilization by 2'-deoxy-2'-fluoro-D-arabinonucleic acids (2'F-ANA).","year":2007}