Bulk and In Situ Quantification of Coniferaldehyde Residues in Lignin. Pesquet, E., Blaschek, L., Takahashi, J., Yamamoto, M., Champagne, A., Nuoendagula, Subbotina, E., Dimotakis, C., Bacisk, Z., & Kajita, S. In Xylem: Methods and Protocols, pages 201–226. Springer US, New York, NY, 2024. ![Bulk and In Situ Quantification of Coniferaldehyde Residues in Lignin [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Lignin is a group of cell wall localised heterophenolic polymers varying in the chemistry of the aromatic and aliphatic parts of its units. The lignin residues common to all vascular plants have an aromatic ring with one para hydroxy group and one meta methoxy group, also called guaiacyl (G). The terminal function of the aliphatic part of these G units, however, varies from alcohols, which are generally abundant, to aldehydes, which represent a smaller proportion of lignin monomers. The proportions of aldehyde to alcohol G units in lignin are, nevertheless, precisely controlled to respond to environmental and development cues. These G aldehyde to alcohol unit proportions differ between each cell wall layer of each cell type to fine-tune the cell wall biomechanical and physico-chemical properties. To precisely determine changes in lignin composition, we, herein, describe the various methods to detect and quantify the levels and positions of G aldehyde units, also called coniferaldehyde residues, of lignin polymers in ground plant samples as well as in situ in histological cross-sections.
@incollection{pesquet_bulk_2024,
address = {New York, NY},
title = {Bulk and {In} {Situ} {Quantification} of {Coniferaldehyde} {Residues} in {Lignin}},
isbn = {978-1-0716-3477-6},
url = {https://doi.org/10.1007/978-1-0716-3477-6_14},
doi = {10.1007/978-1-0716-3477-6_14},
abstract = {Lignin is a group of cell wall localised heterophenolic polymers varying in the chemistry of the aromatic and aliphatic parts of its units. The lignin residues common to all vascular plants have an aromatic ring with one para hydroxy group and one meta methoxy group, also called guaiacyl (G). The terminal function of the aliphatic part of these G units, however, varies from alcohols, which are generally abundant, to aldehydes, which represent a smaller proportion of lignin monomers. The proportions of aldehyde to alcohol G units in lignin are, nevertheless, precisely controlled to respond to environmental and development cues. These G aldehyde to alcohol unit proportions differ between each cell wall layer of each cell type to fine-tune the cell wall biomechanical and physico-chemical properties. To precisely determine changes in lignin composition, we, herein, describe the various methods to detect and quantify the levels and positions of G aldehyde units, also called coniferaldehyde residues, of lignin polymers in ground plant samples as well as in situ in histological cross-sections.},
language = {en},
urldate = {2026-01-30},
booktitle = {Xylem: {Methods} and {Protocols}},
publisher = {Springer US},
author = {Pesquet, Edouard and Blaschek, Leonard and Takahashi, Junko and Yamamoto, Masanobu and Champagne, Antoine and {Nuoendagula} and Subbotina, Elena and Dimotakis, Charilaos and Bacisk, Zoltán and Kajita, Shinya},
editor = {Agusti, Javier},
year = {2024},
keywords = {Coniferaldehyde residues, In situ quantitative chemical imaging, Lignin, Pyrolysis-GC/MS, Raman microspectroscopy, Thioacidolysis-GC/MS, Wiesner test, Xylem cell types},
pages = {201--226},
}
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The terminal function of the aliphatic part of these G units, however, varies from alcohols, which are generally abundant, to aldehydes, which represent a smaller proportion of lignin monomers. The proportions of aldehyde to alcohol G units in lignin are, nevertheless, precisely controlled to respond to environmental and development cues. These G aldehyde to alcohol unit proportions differ between each cell wall layer of each cell type to fine-tune the cell wall biomechanical and physico-chemical properties. To precisely determine changes in lignin composition, we, herein, describe the various methods to detect and quantify the levels and positions of G aldehyde units, also called coniferaldehyde residues, of lignin polymers in ground plant samples as well as in situ in histological cross-sections.","language":"en","urldate":"2026-01-30","booktitle":"Xylem: Methods and Protocols","publisher":"Springer US","author":[{"propositions":[],"lastnames":["Pesquet"],"firstnames":["Edouard"],"suffixes":[]},{"propositions":[],"lastnames":["Blaschek"],"firstnames":["Leonard"],"suffixes":[]},{"propositions":[],"lastnames":["Takahashi"],"firstnames":["Junko"],"suffixes":[]},{"propositions":[],"lastnames":["Yamamoto"],"firstnames":["Masanobu"],"suffixes":[]},{"propositions":[],"lastnames":["Champagne"],"firstnames":["Antoine"],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Nuoendagula"],"suffixes":[]},{"propositions":[],"lastnames":["Subbotina"],"firstnames":["Elena"],"suffixes":[]},{"propositions":[],"lastnames":["Dimotakis"],"firstnames":["Charilaos"],"suffixes":[]},{"propositions":[],"lastnames":["Bacisk"],"firstnames":["Zoltán"],"suffixes":[]},{"propositions":[],"lastnames":["Kajita"],"firstnames":["Shinya"],"suffixes":[]}],"editor":[{"propositions":[],"lastnames":["Agusti"],"firstnames":["Javier"],"suffixes":[]}],"year":"2024","keywords":"Coniferaldehyde residues, In situ quantitative chemical imaging, Lignin, Pyrolysis-GC/MS, Raman microspectroscopy, Thioacidolysis-GC/MS, Wiesner test, Xylem cell types","pages":"201–226","bibtex":"@incollection{pesquet_bulk_2024,\n\taddress = {New York, NY},\n\ttitle = {Bulk and {In} {Situ} {Quantification} of {Coniferaldehyde} {Residues} in {Lignin}},\n\tisbn = {978-1-0716-3477-6},\n\turl = {https://doi.org/10.1007/978-1-0716-3477-6_14},\n\tdoi = {10.1007/978-1-0716-3477-6_14},\n\tabstract = {Lignin is a group of cell wall localised heterophenolic polymers varying in the chemistry of the aromatic and aliphatic parts of its units. 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