Design of stable α-helices using global sequence optimization. Petukhov, M., Tatsu, Y., Tamaki, K., Murase, S., Uekawa, H., Yoshikawa, S., Serrano, L., & Yumoto, N. Journal of Peptide Science, 15(5):359-365, 2009. cited By 10![Design of stable α-helices using global sequence optimization [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The rational design of peptide and protein helices is not only of practical importance for protein engineering but also is a useful approach in attempts to improve our understanding of protein folding. Recent modifications of theoreticalmodels of helix-coil transitions allow accurate predictions of the helix stability of monomeric peptides in water and provide new possibilities for protein design. We report here a new method for the design of α-helices in peptides and proteins using AGADIR, the statistical mechanical theory for helix-coil transitions in monomeric peptides and the tunneling algorithm of global optimization of multidimensional functions for optimization of amino acid sequences. CD measurements of helical content of peptides with optimized sequences indicate that the helical potential of protein amino acids is high enough to allow formation of stable α-helices in peptides as short as of 10 residues in length. The results show the maximum achievable helix content (HC) of short peptides with fully optimized sequences at 5 °C is expected to be ∼70-75%. Under certain conditions the method can be a powerful practical tool for protein engineering. Unlike traditional approaches that are often used to increase protein stability by adding a few favorable interactions to the protein structure, this method deals with all possible sequences of protein helices and selects the best one from them. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd.
@ARTICLE{Petukhov2009359,
author={Petukhov, M. and Tatsu, Y. and Tamaki, K. and Murase, S. and Uekawa, H. and Yoshikawa, S. and Serrano, L. and Yumoto, N.},
title={Design of stable α-helices using global sequence optimization},
journal={Journal of Peptide Science},
year={2009},
volume={15},
number={5},
pages={359-365},
doi={10.1002/psc.1122},
note={cited By 10},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-67649171707&doi=10.1002%2fpsc.1122&partnerID=40&md5=ee949545d1639a86bef8ca445e875365},
affiliation={Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, 188300 Gatchina, Russian Federation; National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka 563-8577, Japan; CRG-EMBL Systems Biology Unit, Centre de Regulacio Genomica, Dr Aiguader 88, 08003 Barcelona, Spain},
abstract={The rational design of peptide and protein helices is not only of practical importance for protein engineering but also is a useful approach in attempts to improve our understanding of protein folding. Recent modifications of theoreticalmodels of helix-coil transitions allow accurate predictions of the helix stability of monomeric peptides in water and provide new possibilities for protein design. We report here a new method for the design of α-helices in peptides and proteins using AGADIR, the statistical mechanical theory for helix-coil transitions in monomeric peptides and the tunneling algorithm of global optimization of multidimensional functions for optimization of amino acid sequences. CD measurements of helical content of peptides with optimized sequences indicate that the helical potential of protein amino acids is high enough to allow formation of stable α-helices in peptides as short as of 10 residues in length. The results show the maximum achievable helix content (HC) of short peptides with fully optimized sequences at 5 °C is expected to be ∼70-75%. Under certain conditions the method can be a powerful practical tool for protein engineering. Unlike traditional approaches that are often used to increase protein stability by adding a few favorable interactions to the protein structure, this method deals with all possible sequences of protein helices and selects the best one from them. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd.},
author_keywords={α-helix; AGADIR; Design; Sequence optimization; Tunneling algorithm},
correspondence_address1={Petukhov, M.; Department of Molecular and Radiation Biophysics, Petersburg Institute of Nuclear Physics, RAS, Orlovarosha, Gatchina 88300, Russian Federation; email: michael.petukhov@gmail.com},
issn={10752617},
coden={JPSIE},
pubmed_id={19222027},
language={English},
abbrev_source_title={J. Pept. Sci.},
document_type={Article},
source={Scopus},
}
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