Aging Cell Culture. Phipps, S. M. O., Berletch, J. B., Andrews, L. G., & Tollefsbol, T. O. Methods in molecular biology (Clifton, N.J.), 371:9–19, 2007.
Aging Cell Culture [link]Paper  abstract   bibtex   
Culturing and subcultivation of normal human diploid fibroblasts have advanced our understanding of the molecular events involved in aging. This progress is leading to the development of therapies that slow or ablate the adverse physiological and pathological changes associated with aging. It has been established that normal human diploid fibro-blasts can proliferate in culture for only finite periods of time. Hayflick and Moorhead and others have described numerous types of cells, ranging from fetal to adult, that were incapable of indefinite proliferation. There are many ways to study aging in vitro, and this chapter summarizes some laboratory procedures.
@article{phipps_aging_2007,
	title = {Aging {Cell} {Culture}},
	volume = {371},
	issn = {1064-3745},
	url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2423218/},
	abstract = {Culturing and subcultivation of normal human diploid fibroblasts have advanced our understanding of the molecular events involved in aging. This progress is leading to the development of therapies that slow or ablate the adverse physiological and pathological changes associated with aging. It has been established that normal human diploid fibro-blasts can proliferate in culture for only finite periods of time. Hayflick and Moorhead and others have described numerous types of cells, ranging from fetal to adult, that were incapable of indefinite proliferation. There are many ways to study aging in vitro, and this chapter summarizes some laboratory procedures.},
	urldate = {2022-08-03},
	journal = {Methods in molecular biology (Clifton, N.J.)},
	author = {Phipps, Sharla M. O. and Berletch, Joel B. and Andrews, Lucy G. and Tollefsbol, Trygve O.},
	year = {2007},
	pmid = {17634570},
	pmcid = {PMC2423218},
	pages = {9--19},
}

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