@article{
 title = {Survey in the sugarcane expressed sequence tag database (SUCEST) for simple sequence repeats},
 type = {article},
 year = {2004},
 identifiers = {[object Object]},
 keywords = {Expressed sequence tags (ESTs),Genetic mapping,Microsatellites (SSRs),Polyploid,Sugarcane},
 pages = {795-804},
 volume = {47},
 id = {3644cced-0fbf-31b9-8084-17beccc9f699},
 created = {2015-11-22T13:21:24.000Z},
 file_attached = {false},
 profile_id = {e36cbae0-8a6b-3799-b43a-920a55c297dc},
 last_modified = {2015-11-22T13:21:24.000Z},
 read = {false},
 starred = {false},
 authored = {true},
 confirmed = {false},
 hidden = {false},
 abstract = {Sugarcane microsatellites or simple sequence repeats (SSR) were developed in an economical and practical way by mining EST databases. A survey in the SUCEST (sugarcane EST) database revealed a total of 2005 clusters out of 43 141 containing SSRs. Of these, 8.2% were dinucleotide, 30.5% were trinucleotide, and 61.3% were tetranucleotide repeats. Except for dinucleotides, the CG-rich motif types were the most common. Differences in abundance of trinucleotide motif types were observed between EST-SSRs and those isolated from sugarcane genomic libraries. Among the different cDNA libraries used for EST sequencing, SSRs were more frequent in the ones derived from leaf roll (LR). Twenty-three out of 30 tested SSRs produced scorable polymorphisms in 18 sugarcane commercial clones. These EST-SSRs showed a moderate level of polymorphism with some SSRs producing unique fingerprints. The number of alleles observed among the 18 clones evaluated varied from 2 to 15, with an average of 6.04 alleles/locus. The polymorphism information content (PIC) values ranged from 0.28 to 0.90 with a mean of 0.66. The EST-SSRs screened over both parents (SP 80-180; SP 80-4966) and 6 F1 individuals produced 52 segregating markers that could potentially be used for sugarcane mapping. The EST-SSRs were found in clusters that had significant homology to proteins involved in important metabolic pathways such as sugar biosynthesis, proving that EST-SSRs are a valuable tool for the construction of a functional sugarcane map.},
 bibtype = {article},
 author = {Pinto, L.R. and Oliveira, K.M. and Ulian, E.C. and Garcia, A.A.F. and De Souza, A.P.},
 journal = {Genome},
 number = {5}
}

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The EST-SSRs screened over both parents (SP 80-180; SP 80-4966) and 6 F1 individuals produced 52 segregating markers that could potentially be used for sugarcane mapping. 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