Circumferential esophageal replacement using a tube-shaped tissue-engineered substitute: An experimental study in minipigs. Poghosyan, T., Sfeir, R., Michaud, L., Bruneval, P., Domet, T., Vanneaux, V., Luong-Nguyen, M., Gaujoux, S., Gottrand, F., Larghero, J., & Cattan, P. Surgery, 158(1):266–277, July, 2015. ![Circumferential esophageal replacement using a tube-shaped tissue-engineered substitute: An experimental study in minipigs [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex BACKGROUND: Esophageal replacement by the colon or the stomach for malignant and nonmalignant esophageal diseases exposes to significant morbidity and mortality. In this setting, tissue engineering seems to be a seductive alternative. METHODS: In a porcine model, we performed a 5-cm-long circumferential replacement of the cervical esophagus by a tubulized acellular matrix (small intestinal submucosa) cellularized with autologous skeletal myoblasts and covered by a human amniotic membrane seeded with autologous oral epithelial cells. The substitute was grown for 2 weeks in the great omentum before esophageal replacement. Eighteen minipigs (divided into 3 groups: group A [substitute with esophageal endoprothesis; n = 6], group B [substitute alone; n = 6], and group C [endoprothesis alone; n = 6]) were included. The esophageal endoprothesis was removed at 6 months. Animals were killed sequentially over a 12 month-period. Clinical, endoscopic, radiologic and histologic outcomes were analyzed. RESULTS: All animals except 1 of in groups B and C died during the first 2 months owing to refractory esophageal stenosis or endoprothesis extrusion. Nutritional autonomy without endoprothesis was observed in all animals of group A with a follow-up of \textgreater6 months (n = 3). A phenotype similar to that of native esophagus, consisting of a mature epithelium, submucosal glands, and a circular muscular layer, was observed after 9 months. CONCLUSION: In this model, the circumferential replacement of the cervical esophagus by a tube-shaped tissue-engineered substitute under the temporary cover of an esophageal endoprothesis allowed nutritional autonomy and tissue remodeling toward an esophageal phenotype.
@article{poghosyan_circumferential_2015,
title = {Circumferential esophageal replacement using a tube-shaped tissue-engineered substitute: {An} experimental study in minipigs},
volume = {158},
issn = {1532-7361},
shorttitle = {Circumferential esophageal replacement using a tube-shaped tissue-engineered substitute},
url = {http://hdl.handle.net/20.500.12210/4703},
doi = {10.1016/j.surg.2015.01.020},
abstract = {BACKGROUND: Esophageal replacement by the colon or the stomach for malignant and nonmalignant esophageal diseases exposes to significant morbidity and mortality. In this setting, tissue engineering seems to be a seductive alternative.
METHODS: In a porcine model, we performed a 5-cm-long circumferential replacement of the cervical esophagus by a tubulized acellular matrix (small intestinal submucosa) cellularized with autologous skeletal myoblasts and covered by a human amniotic membrane seeded with autologous oral epithelial cells. The substitute was grown for 2 weeks in the great omentum before esophageal replacement. Eighteen minipigs (divided into 3 groups: group A [substitute with esophageal endoprothesis; n = 6], group B [substitute alone; n = 6], and group C [endoprothesis alone; n = 6]) were included. The esophageal endoprothesis was removed at 6 months. Animals were killed sequentially over a 12 month-period. Clinical, endoscopic, radiologic and histologic outcomes were analyzed.
RESULTS: All animals except 1 of in groups B and C died during the first 2 months owing to refractory esophageal stenosis or endoprothesis extrusion. Nutritional autonomy without endoprothesis was observed in all animals of group A with a follow-up of {\textgreater}6 months (n = 3). A phenotype similar to that of native esophagus, consisting of a mature epithelium, submucosal glands, and a circular muscular layer, was observed after 9 months.
CONCLUSION: In this model, the circumferential replacement of the cervical esophagus by a tube-shaped tissue-engineered substitute under the temporary cover of an esophageal endoprothesis allowed nutritional autonomy and tissue remodeling toward an esophageal phenotype.},
language = {eng},
number = {1},
journal = {Surgery},
author = {Poghosyan, Tigran and Sfeir, Rony and Michaud, Laurent and Bruneval, Patrick and Domet, Thomas and Vanneaux, Valerie and Luong-Nguyen, Minh and Gaujoux, Sebastien and Gottrand, Frederic and Larghero, Jerome and Cattan, Pierre},
month = jul,
year = {2015},
keywords = {Animals, Artificial Organs, Esophagus, Intestine, Small, Male, Models, Animal, Swine, Swine, Miniature, Tissue Engineering, Tissue Scaffolds},
pages = {266--277},
}
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In this setting, tissue engineering seems to be a seductive alternative. METHODS: In a porcine model, we performed a 5-cm-long circumferential replacement of the cervical esophagus by a tubulized acellular matrix (small intestinal submucosa) cellularized with autologous skeletal myoblasts and covered by a human amniotic membrane seeded with autologous oral epithelial cells. The substitute was grown for 2 weeks in the great omentum before esophageal replacement. Eighteen minipigs (divided into 3 groups: group A [substitute with esophageal endoprothesis; n = 6], group B [substitute alone; n = 6], and group C [endoprothesis alone; n = 6]) were included. The esophageal endoprothesis was removed at 6 months. Animals were killed sequentially over a 12 month-period. Clinical, endoscopic, radiologic and histologic outcomes were analyzed. RESULTS: All animals except 1 of in groups B and C died during the first 2 months owing to refractory esophageal stenosis or endoprothesis extrusion. Nutritional autonomy without endoprothesis was observed in all animals of group A with a follow-up of \\textgreater6 months (n = 3). A phenotype similar to that of native esophagus, consisting of a mature epithelium, submucosal glands, and a circular muscular layer, was observed after 9 months. CONCLUSION: In this model, the circumferential replacement of the cervical esophagus by a tube-shaped tissue-engineered substitute under the temporary cover of an esophageal endoprothesis allowed nutritional autonomy and tissue remodeling toward an esophageal phenotype.","language":"eng","number":"1","journal":"Surgery","author":[{"propositions":[],"lastnames":["Poghosyan"],"firstnames":["Tigran"],"suffixes":[]},{"propositions":[],"lastnames":["Sfeir"],"firstnames":["Rony"],"suffixes":[]},{"propositions":[],"lastnames":["Michaud"],"firstnames":["Laurent"],"suffixes":[]},{"propositions":[],"lastnames":["Bruneval"],"firstnames":["Patrick"],"suffixes":[]},{"propositions":[],"lastnames":["Domet"],"firstnames":["Thomas"],"suffixes":[]},{"propositions":[],"lastnames":["Vanneaux"],"firstnames":["Valerie"],"suffixes":[]},{"propositions":[],"lastnames":["Luong-Nguyen"],"firstnames":["Minh"],"suffixes":[]},{"propositions":[],"lastnames":["Gaujoux"],"firstnames":["Sebastien"],"suffixes":[]},{"propositions":[],"lastnames":["Gottrand"],"firstnames":["Frederic"],"suffixes":[]},{"propositions":[],"lastnames":["Larghero"],"firstnames":["Jerome"],"suffixes":[]},{"propositions":[],"lastnames":["Cattan"],"firstnames":["Pierre"],"suffixes":[]}],"month":"July","year":"2015","keywords":"Animals, Artificial Organs, Esophagus, Intestine, Small, Male, Models, Animal, Swine, Swine, Miniature, Tissue Engineering, Tissue Scaffolds","pages":"266–277","bibtex":"@article{poghosyan_circumferential_2015,\n\ttitle = {Circumferential esophageal replacement using a tube-shaped tissue-engineered substitute: {An} experimental study in minipigs},\n\tvolume = {158},\n\tissn = {1532-7361},\n\tshorttitle = {Circumferential esophageal replacement using a tube-shaped tissue-engineered substitute},\n\turl = {http://hdl.handle.net/20.500.12210/4703},\n\tdoi = {10.1016/j.surg.2015.01.020},\n\tabstract = {BACKGROUND: Esophageal replacement by the colon or the stomach for malignant and nonmalignant esophageal diseases exposes to significant morbidity and mortality. In this setting, tissue engineering seems to be a seductive alternative.\nMETHODS: In a porcine model, we performed a 5-cm-long circumferential replacement of the cervical esophagus by a tubulized acellular matrix (small intestinal submucosa) cellularized with autologous skeletal myoblasts and covered by a human amniotic membrane seeded with autologous oral epithelial cells. The substitute was grown for 2 weeks in the great omentum before esophageal replacement. Eighteen minipigs (divided into 3 groups: group A [substitute with esophageal endoprothesis; n = 6], group B [substitute alone; n = 6], and group C [endoprothesis alone; n = 6]) were included. The esophageal endoprothesis was removed at 6 months. Animals were killed sequentially over a 12 month-period. Clinical, endoscopic, radiologic and histologic outcomes were analyzed.\nRESULTS: All animals except 1 of in groups B and C died during the first 2 months owing to refractory esophageal stenosis or endoprothesis extrusion. Nutritional autonomy without endoprothesis was observed in all animals of group A with a follow-up of {\\textgreater}6 months (n = 3). A phenotype similar to that of native esophagus, consisting of a mature epithelium, submucosal glands, and a circular muscular layer, was observed after 9 months.\nCONCLUSION: In this model, the circumferential replacement of the cervical esophagus by a tube-shaped tissue-engineered substitute under the temporary cover of an esophageal endoprothesis allowed nutritional autonomy and tissue remodeling toward an esophageal phenotype.},\n\tlanguage = {eng},\n\tnumber = {1},\n\tjournal = {Surgery},\n\tauthor = {Poghosyan, Tigran and Sfeir, Rony and Michaud, Laurent and Bruneval, Patrick and Domet, Thomas and Vanneaux, Valerie and Luong-Nguyen, Minh and Gaujoux, Sebastien and Gottrand, Frederic and Larghero, Jerome and Cattan, Pierre},\n\tmonth = jul,\n\tyear = {2015},\n\tkeywords = {Animals, Artificial Organs, Esophagus, Intestine, Small, Male, Models, Animal, Swine, Swine, Miniature, Tissue Engineering, Tissue Scaffolds},\n\tpages = {266--277},\n}\n\n\n\n","author_short":["Poghosyan, T.","Sfeir, R.","Michaud, L.","Bruneval, P.","Domet, T.","Vanneaux, V.","Luong-Nguyen, M.","Gaujoux, S.","Gottrand, F.","Larghero, J.","Cattan, P."],"key":"poghosyan_circumferential_2015","id":"poghosyan_circumferential_2015","bibbaseid":"poghosyan-sfeir-michaud-bruneval-domet-vanneaux-luongnguyen-gaujoux-etal-circumferentialesophagealreplacementusingatubeshapedtissueengineeredsubstituteanexperimentalstudyinminipigs-2015","role":"author","urls":{"Paper":"http://hdl.handle.net/20.500.12210/4703"},"keyword":["Animals","Artificial Organs","Esophagus","Intestine","Small","Male","Models","Animal","Swine","Swine","Miniature","Tissue Engineering","Tissue Scaffolds"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero-group/Leromela/5826776","dataSources":["xEepG2Bu5H8yCXNLe","9KDMJQgGj9aC6ujCM","tJu2LQsng3WaoKpcL","epXRk2zQpWZQCqqGz"],"keywords":["animals","artificial organs","esophagus","intestine","small","male","models","animal","swine","swine","miniature","tissue engineering","tissue scaffolds"],"search_terms":["circumferential","esophageal","replacement","using","tube","shaped","tissue","engineered","substitute","experimental","study","minipigs","poghosyan","sfeir","michaud","bruneval","domet","vanneaux","luong-nguyen","gaujoux","gottrand","larghero","cattan"],"title":"Circumferential esophageal replacement using a tube-shaped tissue-engineered substitute: An experimental study in minipigs","year":2015}