Dioxygenase-encoding AtDAO1 gene controls IAA oxidation and homeostasis in Arabidopsis

Dioxygenase-encoding AtDAO1 gene controls IAA oxidation and homeostasis in Arabidopsis. Porco, S., Pěnčík, A., Rashed, A., Voß, U., Casanova-Sáez, R., Bishopp, A., Golebiowska, A., Bhosale, R., Swarup, R., Swarup, K., Peňáková, P., Novák, O., Staswick, P., Hedden, P., Phillips, A. L., Vissenberg, K., Bennett, M. J., & Ljung, K. Proceedings of the National Academy of Sciences, 113(39):11016–11021, September, 2016.

Paper doi abstract bibtex

Auxin represents a key signal in plants, regulating almost every aspect of their growth and development. Major breakthroughs have been made dissecting the molecular basis of auxin transport, perception, and response. In contrast, how plants control the metabolism and homeostasis of the major form of auxin in plants, indole-3-acetic acid (IAA), remains unclear. In this paper, we initially describe the function of the Arabidopsis thaliana gene DIOXYGENASE FOR AUXIN OXIDATION 1 ( AtDAO1 ). Transcriptional and translational reporter lines revealed that AtDAO1 encodes a highly root-expressed, cytoplasmically localized IAA oxidase. Stable isotope-labeled IAA feeding studies of loss and gain of function AtDAO1 lines showed that this oxidase represents the major regulator of auxin degradation to 2-oxoindole-3-acetic acid (oxIAA) in Arabidopsis . Surprisingly, AtDAO1 loss and gain of function lines exhibited relatively subtle auxin-related phenotypes, such as altered root hair length. Metabolite profiling of mutant lines revealed that disrupting AtDAO1 regulation resulted in major changes in steady-state levels of oxIAA and IAA conjugates but not IAA. Hence, IAA conjugation and catabolism seem to regulate auxin levels in Arabidopsis in a highly redundant manner. We observed that transcripts of AtDOA1 IAA oxidase and GH3 IAA-conjugating enzymes are auxin-inducible, providing a molecular basis for their observed functional redundancy. We conclude that the AtDAO1 gene plays a key role regulating auxin homeostasis in Arabidopsis , acting in concert with GH3 genes, to maintain auxin concentration at optimal levels for plant growth and development.

@article{porco_dioxygenase-encoding_2016,
	title = {Dioxygenase-encoding \textit{{AtDAO1}} gene controls {IAA} oxidation and homeostasis in \textit{{Arabidopsis}}},
	volume = {113},
	issn = {0027-8424, 1091-6490},
	url = {http://www.pnas.org/lookup/doi/10.1073/pnas.1604375113},
	doi = {10/f3t58q},
	abstract = {Auxin represents a key signal in plants, regulating almost every aspect of their growth and development. Major breakthroughs have been made dissecting the molecular basis of auxin transport, perception, and response. In contrast, how plants control the metabolism and homeostasis of the major form of auxin in plants, indole-3-acetic acid (IAA), remains unclear. In this paper, we initially describe the function of the
              Arabidopsis thaliana
              gene
              DIOXYGENASE FOR AUXIN OXIDATION 1
              (
              AtDAO1
              ). Transcriptional and translational reporter lines revealed that
              AtDAO1
              encodes a highly root-expressed, cytoplasmically localized IAA oxidase. Stable isotope-labeled IAA feeding studies of loss and gain of function
              AtDAO1
              lines showed that this oxidase represents the major regulator of auxin degradation to 2-oxoindole-3-acetic acid (oxIAA) in
              Arabidopsis
              . Surprisingly,
              AtDAO1
              loss and gain of function lines exhibited relatively subtle auxin-related phenotypes, such as altered root hair length. Metabolite profiling of mutant lines revealed that disrupting
              AtDAO1
              regulation resulted in major changes in steady-state levels of oxIAA and IAA conjugates but not IAA. Hence, IAA conjugation and catabolism seem to regulate auxin levels in
              Arabidopsis
              in a highly redundant manner. We observed that transcripts of
              AtDOA1
              IAA oxidase and
              GH3
              IAA-conjugating enzymes are auxin-inducible, providing a molecular basis for their observed functional redundancy. We conclude that the
              AtDAO1
              gene plays a key role regulating auxin homeostasis in
              Arabidopsis
              , acting in concert with
              GH3
              genes, to maintain auxin concentration at optimal levels for plant growth and development.},
	language = {en},
	number = {39},
	urldate = {2021-06-07},
	journal = {Proceedings of the National Academy of Sciences},
	author = {Porco, Silvana and Pěnčík, Aleš and Rashed, Afaf and Voß, Ute and Casanova-Sáez, Rubén and Bishopp, Anthony and Golebiowska, Agata and Bhosale, Rahul and Swarup, Ranjan and Swarup, Kamal and Peňáková, Pavlína and Novák, Ondřej and Staswick, Paul and Hedden, Peter and Phillips, Andrew L. and Vissenberg, Kris and Bennett, Malcolm J. and Ljung, Karin},
	month = sep,
	year = {2016},
	pages = {11016--11021},
}

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Major breakthroughs have been made dissecting the molecular basis of auxin transport, perception, and response. In contrast, how plants control the metabolism and homeostasis of the major form of auxin in plants, indole-3-acetic acid (IAA), remains unclear. In this paper, we initially describe the function of the\n Arabidopsis thaliana\n gene\n DIOXYGENASE FOR AUXIN OXIDATION 1\n (\n AtDAO1\n ). Transcriptional and translational reporter lines revealed that\n AtDAO1\n encodes a highly root-expressed, cytoplasmically localized IAA oxidase. Stable isotope-labeled IAA feeding studies of loss and gain of function\n AtDAO1\n lines showed that this oxidase represents the major regulator of auxin degradation to 2-oxoindole-3-acetic acid (oxIAA) in\n Arabidopsis\n . Surprisingly,\n AtDAO1\n loss and gain of function lines exhibited relatively subtle auxin-related phenotypes, such as altered root hair length. 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