Processing of 3'-phosphoglycolate-terminated DNA double strand breaks by Artemis nuclease. Povirk, L. F., Zhou, T., Zhou, R., Cowan, M. J., & Yannone, S. M. The Journal of Biological Chemistry, 282(6):3547–3558, February, 2007. doi abstract bibtex The Artemis nuclease is required for V(D)J recombination and for repair of an as yet undefined subset of radiation-induced DNA double strand breaks. To assess the possibility that Artemis acts on oxidatively modified double strand break termini, its activity toward model DNA substrates, bearing either 3'-hydroxyl or 3'-phosphoglycolate moieties, was examined. A 3'-phosphoglycolate had little effect on Artemis-mediated trimming of long 3' overhangs (\textgreater or =9 nucleotides), which were efficiently trimmed to 4-5 nucleotides. However, 3'-phosphoglycolates on overhangs of 4-5 bases promoted Artemis-mediated removal of a single 3'-terminal nucleotide, while at least 2 nucleotides were trimmed from identical hydroxyl-terminated substrates. Artemis also efficiently removed a single nucleotide from a phosphoglycolate-terminated 3-base 3' overhang, while leaving an analogous hydroxyl-terminated overhang largely intact. Such removal was completely dependent on DNA-dependent protein kinase and ATP and was largely dependent on Ku, which markedly stimulated Artemis activity toward all 3' overhangs. Together, these data suggest that efficient Artemis-mediated cleavage of 3' overhangs requires a minimum of 2 nucleotides, or a nucleotide plus a phosphoglycolate, 3' to the cleavage site, as well as 2 unpaired nucleotides 5' to the cleavage site. Shorter 3'-phosphoglycolate-terminated overhangs and blunt ends were also processed by Artemis but much more slowly. Consistent with a role for Artemis in repair of terminally blocked double strand breaks in vivo, human cells lacking Artemis exhibited hypersensitivity to x-rays, bleomycin, and neocarzinostatin, which all induce 3'-phosphoglycolate-terminated double strand breaks.
@article{povirk_processing_2007,
title = {Processing of 3'-phosphoglycolate-terminated {DNA} double strand breaks by {Artemis} nuclease},
volume = {282},
issn = {0021-9258},
doi = {10.1074/jbc.M607745200},
abstract = {The Artemis nuclease is required for V(D)J recombination and for repair of an as yet undefined subset of radiation-induced DNA double strand breaks. To assess the possibility that Artemis acts on oxidatively modified double strand break termini, its activity toward model DNA substrates, bearing either 3'-hydroxyl or 3'-phosphoglycolate moieties, was examined. A 3'-phosphoglycolate had little effect on Artemis-mediated trimming of long 3' overhangs ({\textgreater} or =9 nucleotides), which were efficiently trimmed to 4-5 nucleotides. However, 3'-phosphoglycolates on overhangs of 4-5 bases promoted Artemis-mediated removal of a single 3'-terminal nucleotide, while at least 2 nucleotides were trimmed from identical hydroxyl-terminated substrates. Artemis also efficiently removed a single nucleotide from a phosphoglycolate-terminated 3-base 3' overhang, while leaving an analogous hydroxyl-terminated overhang largely intact. Such removal was completely dependent on DNA-dependent protein kinase and ATP and was largely dependent on Ku, which markedly stimulated Artemis activity toward all 3' overhangs. Together, these data suggest that efficient Artemis-mediated cleavage of 3' overhangs requires a minimum of 2 nucleotides, or a nucleotide plus a phosphoglycolate, 3' to the cleavage site, as well as 2 unpaired nucleotides 5' to the cleavage site. Shorter 3'-phosphoglycolate-terminated overhangs and blunt ends were also processed by Artemis but much more slowly. Consistent with a role for Artemis in repair of terminally blocked double strand breaks in vivo, human cells lacking Artemis exhibited hypersensitivity to x-rays, bleomycin, and neocarzinostatin, which all induce 3'-phosphoglycolate-terminated double strand breaks.},
language = {eng},
number = {6},
journal = {The Journal of Biological Chemistry},
author = {Povirk, Lawrence F. and Zhou, Tong and Zhou, Ruizhe and Cowan, Morton J. and Yannone, Steven M.},
month = feb,
year = {2007},
keywords = {Bleomycin, Cell Line, DNA, DNA Damage, DNA Repair, DNA-Activated Protein Kinase, Endodeoxyribonucleases, Free Radicals, Glycolates, Nuclear Proteins, RNA Processing, Post-Transcriptional, X-Rays, Zinostatin},
pages = {3547--3558},
}
Downloads: 0
{"_id":"S8jkSFLQqwbDqe94m","bibbaseid":"povirk-zhou-zhou-cowan-yannone-processingof3phosphoglycolateterminateddnadoublestrandbreaksbyartemisnuclease-2007","author_short":["Povirk, L. F.","Zhou, T.","Zhou, R.","Cowan, M. J.","Yannone, S. M."],"bibdata":{"bibtype":"article","type":"article","title":"Processing of 3'-phosphoglycolate-terminated DNA double strand breaks by Artemis nuclease","volume":"282","issn":"0021-9258","doi":"10.1074/jbc.M607745200","abstract":"The Artemis nuclease is required for V(D)J recombination and for repair of an as yet undefined subset of radiation-induced DNA double strand breaks. To assess the possibility that Artemis acts on oxidatively modified double strand break termini, its activity toward model DNA substrates, bearing either 3'-hydroxyl or 3'-phosphoglycolate moieties, was examined. A 3'-phosphoglycolate had little effect on Artemis-mediated trimming of long 3' overhangs (\\textgreater or =9 nucleotides), which were efficiently trimmed to 4-5 nucleotides. However, 3'-phosphoglycolates on overhangs of 4-5 bases promoted Artemis-mediated removal of a single 3'-terminal nucleotide, while at least 2 nucleotides were trimmed from identical hydroxyl-terminated substrates. Artemis also efficiently removed a single nucleotide from a phosphoglycolate-terminated 3-base 3' overhang, while leaving an analogous hydroxyl-terminated overhang largely intact. Such removal was completely dependent on DNA-dependent protein kinase and ATP and was largely dependent on Ku, which markedly stimulated Artemis activity toward all 3' overhangs. Together, these data suggest that efficient Artemis-mediated cleavage of 3' overhangs requires a minimum of 2 nucleotides, or a nucleotide plus a phosphoglycolate, 3' to the cleavage site, as well as 2 unpaired nucleotides 5' to the cleavage site. Shorter 3'-phosphoglycolate-terminated overhangs and blunt ends were also processed by Artemis but much more slowly. Consistent with a role for Artemis in repair of terminally blocked double strand breaks in vivo, human cells lacking Artemis exhibited hypersensitivity to x-rays, bleomycin, and neocarzinostatin, which all induce 3'-phosphoglycolate-terminated double strand breaks.","language":"eng","number":"6","journal":"The Journal of Biological Chemistry","author":[{"propositions":[],"lastnames":["Povirk"],"firstnames":["Lawrence","F."],"suffixes":[]},{"propositions":[],"lastnames":["Zhou"],"firstnames":["Tong"],"suffixes":[]},{"propositions":[],"lastnames":["Zhou"],"firstnames":["Ruizhe"],"suffixes":[]},{"propositions":[],"lastnames":["Cowan"],"firstnames":["Morton","J."],"suffixes":[]},{"propositions":[],"lastnames":["Yannone"],"firstnames":["Steven","M."],"suffixes":[]}],"month":"February","year":"2007","keywords":"Bleomycin, Cell Line, DNA, DNA Damage, DNA Repair, DNA-Activated Protein Kinase, Endodeoxyribonucleases, Free Radicals, Glycolates, Nuclear Proteins, RNA Processing, Post-Transcriptional, X-Rays, Zinostatin","pages":"3547–3558","bibtex":"@article{povirk_processing_2007,\n\ttitle = {Processing of 3'-phosphoglycolate-terminated {DNA} double strand breaks by {Artemis} nuclease},\n\tvolume = {282},\n\tissn = {0021-9258},\n\tdoi = {10.1074/jbc.M607745200},\n\tabstract = {The Artemis nuclease is required for V(D)J recombination and for repair of an as yet undefined subset of radiation-induced DNA double strand breaks. To assess the possibility that Artemis acts on oxidatively modified double strand break termini, its activity toward model DNA substrates, bearing either 3'-hydroxyl or 3'-phosphoglycolate moieties, was examined. A 3'-phosphoglycolate had little effect on Artemis-mediated trimming of long 3' overhangs ({\\textgreater} or =9 nucleotides), which were efficiently trimmed to 4-5 nucleotides. However, 3'-phosphoglycolates on overhangs of 4-5 bases promoted Artemis-mediated removal of a single 3'-terminal nucleotide, while at least 2 nucleotides were trimmed from identical hydroxyl-terminated substrates. Artemis also efficiently removed a single nucleotide from a phosphoglycolate-terminated 3-base 3' overhang, while leaving an analogous hydroxyl-terminated overhang largely intact. Such removal was completely dependent on DNA-dependent protein kinase and ATP and was largely dependent on Ku, which markedly stimulated Artemis activity toward all 3' overhangs. Together, these data suggest that efficient Artemis-mediated cleavage of 3' overhangs requires a minimum of 2 nucleotides, or a nucleotide plus a phosphoglycolate, 3' to the cleavage site, as well as 2 unpaired nucleotides 5' to the cleavage site. Shorter 3'-phosphoglycolate-terminated overhangs and blunt ends were also processed by Artemis but much more slowly. Consistent with a role for Artemis in repair of terminally blocked double strand breaks in vivo, human cells lacking Artemis exhibited hypersensitivity to x-rays, bleomycin, and neocarzinostatin, which all induce 3'-phosphoglycolate-terminated double strand breaks.},\n\tlanguage = {eng},\n\tnumber = {6},\n\tjournal = {The Journal of Biological Chemistry},\n\tauthor = {Povirk, Lawrence F. and Zhou, Tong and Zhou, Ruizhe and Cowan, Morton J. and Yannone, Steven M.},\n\tmonth = feb,\n\tyear = {2007},\n\tkeywords = {Bleomycin, Cell Line, DNA, DNA Damage, DNA Repair, DNA-Activated Protein Kinase, Endodeoxyribonucleases, Free Radicals, Glycolates, Nuclear Proteins, RNA Processing, Post-Transcriptional, X-Rays, Zinostatin},\n\tpages = {3547--3558},\n}\n\n","author_short":["Povirk, L. F.","Zhou, T.","Zhou, R.","Cowan, M. J.","Yannone, S. M."],"key":"povirk_processing_2007","id":"povirk_processing_2007","bibbaseid":"povirk-zhou-zhou-cowan-yannone-processingof3phosphoglycolateterminateddnadoublestrandbreaksbyartemisnuclease-2007","role":"author","urls":{},"keyword":["Bleomycin","Cell Line","DNA","DNA Damage","DNA Repair","DNA-Activated Protein Kinase","Endodeoxyribonucleases","Free Radicals","Glycolates","Nuclear Proteins","RNA Processing","Post-Transcriptional","X-Rays","Zinostatin"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/tplas","dataSources":["YLgywGcXWx96vKKaB"],"keywords":["bleomycin","cell line","dna","dna damage","dna repair","dna-activated protein kinase","endodeoxyribonucleases","free radicals","glycolates","nuclear proteins","rna processing","post-transcriptional","x-rays","zinostatin"],"search_terms":["processing","phosphoglycolate","terminated","dna","double","strand","breaks","artemis","nuclease","povirk","zhou","zhou","cowan","yannone"],"title":"Processing of 3'-phosphoglycolate-terminated DNA double strand breaks by Artemis nuclease","year":2007}