Factors influencing the growth of <i>Attamyces bromatificus</i>, a symbiont of attine ants. Powell, R. J. & Stradling, D. J. Transactions of the British Mycological Society, 87(2):205–213, September, 1986.
Factors influencing the growth of <i>Attamyces bromatificus</i>, a symbiont of attine ants [link]Paper  doi  abstract   bibtex   
Investigations of conditions for optimum growth of the attine symbiont Attamyces bromatificus revealed that it was inhibited by light and grew best at temperatures between 20 and 25°C, while temperatures above 30° were lethal. Mean gongylidia size remained small in cultures at 15°. The pH of ant-maintained fungus gardens ranged from 4.35 to 5.00 irrespective of the plant material used and showed a trend of increasing acidity with age. Cultures on buffered PDA solid medium showed maximum yields of gongylidia biomass between pH 4.5 and 5.0. The ant metathoracic gland secretions, β-hydroxydecanoic acid and IAA had only a minor indirect influence on growth through pH regulation. In liquid culture, growth form was atypical and lacked gongylidia. The best indices of fungal growth were those of fresh weight and gongylidia production on solid media.
@article{powell_factors_1986,
	title = {Factors influencing the growth of \textit{{Attamyces} bromatificus}, a symbiont of attine ants},
	volume = {87},
	issn = {0007-1536},
	url = {http://www.sciencedirect.com/science/article/pii/S0007153686800225},
	doi = {10.1016/S0007-1536(86)80022-5},
	abstract = {Investigations of conditions for optimum growth of the attine symbiont Attamyces bromatificus revealed that it was inhibited by light and grew best at temperatures between 20 and 25°C, while temperatures above 30° were lethal. Mean gongylidia size remained small in cultures at 15°. The pH of ant-maintained fungus gardens ranged from 4.35 to 5.00 irrespective of the plant material used and showed a trend of increasing acidity with age. Cultures on buffered PDA solid medium showed maximum yields of gongylidia biomass between pH 4.5 and 5.0. The ant metathoracic gland secretions, β-hydroxydecanoic acid and IAA had only a minor indirect influence on growth through pH regulation. In liquid culture, growth form was atypical and lacked gongylidia. The best indices of fungal growth were those of fresh weight and gongylidia production on solid media.},
	number = {2},
	urldate = {2017-12-15},
	journal = {Transactions of the British Mycological Society},
	author = {Powell, R. J. and Stradling, D. J.},
	month = sep,
	year = {1986},
	pages = {205--213}
}

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