Selective Control of Synaptically-Connected Circuit Elements by All-Optical Synapses. Prakash, M., Murphy, J., St Laurent, R., Friedman, N., Crespo, E., Bjorefeldt, A., Pal, A., Bhagat, Y., Kauer, J. A., Shaner, N., Lipscombe, D., Moore, C., & Hochgeschwender, U. Technical Report Neuroscience, November, 2021.
Selective Control of Synaptically-Connected Circuit Elements by All-Optical Synapses [link]Paper  doi  abstract   bibtex   
Understanding percepts, engrams and actions requires methods for selectively modulating synaptic communication between specific subsets of interconnected cells. Here, we develop an approach to control synaptically connected elements using bioluminescent light: Luciferase-generated light, originating from a presynaptic axon terminal, modulates an opsin in its postsynaptic target. Vesicular-localized luciferase is released into the synaptic cleft in response to presynaptic activity, creating a real-time Optical Synapse. Light production is under experimenter-control by introduction of the small molecule luciferin. Signal transmission across this optical synapse is temporally defined by the presence of both the luciferin and presynaptic activity. We validate synaptic Interluminescence by multi-electrode recording in cultured neurons and in mice in vivo. Interluminescence represents a powerful approach to achieve synapse-specific and activity-dependent circuit control during behavior in vivo.
@techreport{prakash_selective_2021,
	type = {preprint},
	title = {Selective {Control} of {Synaptically}-{Connected} {Circuit} {Elements} by {All}-{Optical} {Synapses}},
	url = {http://biorxiv.org/lookup/doi/10.1101/2021.10.29.466531},
	abstract = {Understanding percepts, engrams and actions requires methods for selectively modulating synaptic communication between specific subsets of interconnected cells. Here, we develop an approach to control synaptically connected elements using bioluminescent light: Luciferase-generated light, originating from a presynaptic axon terminal, modulates an opsin in its postsynaptic target. Vesicular-localized luciferase is released into the synaptic cleft in response to presynaptic activity, creating a real-time Optical Synapse. Light production is under experimenter-control by introduction of the small molecule luciferin. Signal transmission across this optical synapse is temporally defined by the presence of both the luciferin and presynaptic activity. We validate synaptic Interluminescence by multi-electrode recording in cultured neurons and in mice in vivo. Interluminescence represents a powerful approach to achieve synapse-specific and activity-dependent circuit control during behavior in vivo.},
	language = {en},
	urldate = {2021-11-03},
	institution = {Neuroscience},
	author = {Prakash, Mansi and Murphy, Jeremy and St Laurent, Robyn and Friedman, Nina and Crespo, Emmanual and Bjorefeldt, Andreas and Pal, Akash and Bhagat, Yuvraj and Kauer, Julie A. and Shaner, Nathan and Lipscombe, Diane and Moore, Christopher and Hochgeschwender, Ute},
	month = nov,
	year = {2021},
	doi = {10.1101/2021.10.29.466531},
	keywords = {BiolumHub},
}

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