Two-photon excitation microscopy using the second singlet state of fluorescent agents within the "tissue optical window". Pu, Y., Shi, L., Pratavieira, S., & Alfano, R. Journal of Applied Physics, 2013. abstract bibtex Two-photon (2P) excitation of the second singlet (S 2 ) state of a group of fluorescent agents with near infrared emission was used to extend the optical excitation and imaging regime of 2P microscope into "tissue optical window" (650-1100 nm). As the first step to achieve deeper optical imaging, Chlorophyll a and Indocyanine green are investigated and demonstrated as imaging agents using 2P S 2 excitation at 800 nm for microscope imaging at 685 nm. The salient feature is to drive both the 2P excitation and emission wavelengths of the imaging agents to fall into the "tissue optical window." © 2013 AIP Publishing LLC.
@article{
title = {Two-photon excitation microscopy using the second singlet state of fluorescent agents within the "tissue optical window"},
type = {article},
year = {2013},
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abstract = {Two-photon (2P) excitation of the second singlet (S 2 ) state of a group of fluorescent agents with near infrared emission was used to extend the optical excitation and imaging regime of 2P microscope into "tissue optical window" (650-1100 nm). As the first step to achieve deeper optical imaging, Chlorophyll a and Indocyanine green are investigated and demonstrated as imaging agents using 2P S 2 excitation at 800 nm for microscope imaging at 685 nm. The salient feature is to drive both the 2P excitation and emission wavelengths of the imaging agents to fall into the "tissue optical window." © 2013 AIP Publishing LLC.},
bibtype = {article},
author = {Pu, Y. and Shi, L. and Pratavieira, S. and Alfano, R.R.},
journal = {Journal of Applied Physics},
number = {15}
}
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