Characterization of a novel H3N6 from a Harbor seal (Phoca vitulina) in the North Pacific. Puryear, W., Canas-Arranz, R., Sawatzki, K., Hill, N., Foss, A., Rouse, N., Pace, C., Burek Huntington, K., van de Guchte, A., Khalil, Z., Gonzalez-Reiche, A. S., Pearl, R., Guerra, S., van Bakel, H., Albrecht, R., Garcia-Sastre, A., & Runstadler, J. Emerging Infectious Diseases, June, 2024. abstract bibtex Influenza A Viruses (IAV) are emerging pathogens of public health concern. Wild birds and mammals are important reservoirs that contribute to the evolving genetic diversity of influenza viruses and represent an important transmission interface that may facilitate mammalian adaptation of IAV. Field surveillance studies are essential for public health preparedness to mitigate concerns of zoonotic influenza. Herein, we report the isolation of an IAV from a harbor seal (Phoca vitulina) in Alaska as the first identification of H3N6 within marine mammals. Analysis of the full genome sequence suggested reassortment of H3N8 and H4N6 circulating in dabbling ducks in the Pacific flyway. Assessment of replication and virulence phenotypes in mouse and ferret models of influenza provided evidence of replication in mammals but resulting in minimal disease. Risk assessment pipelines that combine phylogenetic analysis with in vitro and in vivo characterization in relevant animal models are essential for pandemic preparedness.
@article{puryear_characterization_2024,
title = {Characterization of a novel {H3N6} from a {Harbor} seal ({Phoca} vitulina) in the {North} {Pacific}},
volume = {In review},
abstract = {Influenza A Viruses (IAV) are emerging pathogens of public health concern. Wild birds and mammals are important reservoirs that contribute to the evolving genetic diversity of influenza viruses and represent an important transmission interface that may facilitate mammalian adaptation of IAV. Field surveillance studies are essential for public health preparedness to mitigate concerns of zoonotic influenza. Herein, we report the isolation of an IAV from a harbor seal (Phoca vitulina) in Alaska as the first identification of H3N6 within marine mammals. Analysis of the full genome sequence suggested reassortment of H3N8 and H4N6 circulating in dabbling ducks in the Pacific flyway. Assessment of replication and virulence phenotypes in mouse and ferret models of influenza provided evidence of replication in mammals but resulting in minimal disease. Risk assessment pipelines that combine phylogenetic analysis with in vitro and in vivo characterization in relevant animal models are essential for pandemic preparedness.},
journal = {Emerging Infectious Diseases},
author = {Puryear, Wendy and Canas-Arranz, Rodrigo and Sawatzki, Kaitlin and Hill, Nichola and Foss, Alexa and Rouse, Natalie and Pace, Courtney and Burek Huntington, Kathy and van de Guchte, Adriana and Khalil, Zain and Gonzalez-Reiche, Ana Silvia and Pearl, Rebecca and Guerra, Susana and van Bakel, Harm and Albrecht, Randy and Garcia-Sastre, Adolfo and Runstadler, Jonathan},
month = jun,
year = {2024},
}
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Risk assessment pipelines that combine phylogenetic analysis with in vitro and in vivo characterization in relevant animal models are essential for pandemic preparedness.","journal":"Emerging Infectious Diseases","author":[{"propositions":[],"lastnames":["Puryear"],"firstnames":["Wendy"],"suffixes":[]},{"propositions":[],"lastnames":["Canas-Arranz"],"firstnames":["Rodrigo"],"suffixes":[]},{"propositions":[],"lastnames":["Sawatzki"],"firstnames":["Kaitlin"],"suffixes":[]},{"propositions":[],"lastnames":["Hill"],"firstnames":["Nichola"],"suffixes":[]},{"propositions":[],"lastnames":["Foss"],"firstnames":["Alexa"],"suffixes":[]},{"propositions":[],"lastnames":["Rouse"],"firstnames":["Natalie"],"suffixes":[]},{"propositions":[],"lastnames":["Pace"],"firstnames":["Courtney"],"suffixes":[]},{"propositions":[],"lastnames":["Burek","Huntington"],"firstnames":["Kathy"],"suffixes":[]},{"propositions":["van","de"],"lastnames":["Guchte"],"firstnames":["Adriana"],"suffixes":[]},{"propositions":[],"lastnames":["Khalil"],"firstnames":["Zain"],"suffixes":[]},{"propositions":[],"lastnames":["Gonzalez-Reiche"],"firstnames":["Ana","Silvia"],"suffixes":[]},{"propositions":[],"lastnames":["Pearl"],"firstnames":["Rebecca"],"suffixes":[]},{"propositions":[],"lastnames":["Guerra"],"firstnames":["Susana"],"suffixes":[]},{"propositions":["van"],"lastnames":["Bakel"],"firstnames":["Harm"],"suffixes":[]},{"propositions":[],"lastnames":["Albrecht"],"firstnames":["Randy"],"suffixes":[]},{"propositions":[],"lastnames":["Garcia-Sastre"],"firstnames":["Adolfo"],"suffixes":[]},{"propositions":[],"lastnames":["Runstadler"],"firstnames":["Jonathan"],"suffixes":[]}],"month":"June","year":"2024","bibtex":"@article{puryear_characterization_2024,\n\ttitle = {Characterization of a novel {H3N6} from a {Harbor} seal ({Phoca} vitulina) in the {North} {Pacific}},\n\tvolume = {In review},\n\tabstract = {Influenza A Viruses (IAV) are emerging pathogens of public health concern. Wild birds and mammals are important reservoirs that contribute to the evolving genetic diversity of influenza viruses and represent an important transmission interface that may facilitate mammalian adaptation of IAV. Field surveillance studies are essential for public health preparedness to mitigate concerns of zoonotic influenza. Herein, we report the isolation of an IAV from a harbor seal (Phoca vitulina) in Alaska as the first identification of H3N6 within marine mammals. Analysis of the full genome sequence suggested reassortment of H3N8 and H4N6 circulating in dabbling ducks in the Pacific flyway. Assessment of replication and virulence phenotypes in mouse and ferret models of influenza provided evidence of replication in mammals but resulting in minimal disease. 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