Differentiation of mouse EPL (early primitive ectoderm like) cells into endothelial progenitors. Rao, R., Lyons, I, & Stice, S. In SECOND JOINT EMBS-BMES CONFERENCE 2002, VOLS 1-3, CONFERENCE PROCEEDINGS: BIOENGINEERING - INTEGRATIVE METHODOLOGIES, NEW TECHNOLOGIES, of PROCEEDINGS OF ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, pages 682–683, 2002. Engn Med & Biol Soc; Biomed Engn Soc; Natl Sci Fdn; Natl Inst Hlth; Natl Inst Biomed Imaging & Bioengn; Whitaker Fdn. ISSN: 1094-687Xabstract bibtex Embryonic stem cell (ES) based therapies have gained significant interest due to their limitless self-renewal capabilities and potential formation of all tissue types. There is specific interest in our laboratory to produce endothelial cells (EC) from ES cells. Differentiation parameters that have been adapted have focused on the role of the vascular endothelial growth factor (VEGF). Recent studies and work done in our laboratory indicate that mouse ES cells, when grown on a collagen matrix, differentiate into EC progenitors as indicated by production of Flk1(+) (VEGF-receptor) cells. We have a novel technique for culturing stable early primitive ectoderm like (EPL) cells, which are obligatory intermediates in differentiation pathways, and derived from ES cells. Mouse EPL cells were grown on a collagen matrix and Flk1 expression was monitored over an 8-day time period. An early and increased production of Flk1(+) cells when mouse ES cells are converted to EPL cells, was observed. When EPL cells were also exposed to VEGF, endothelial tube structures started to form and networks of tubes were present after 4 days of VEGF in the culture medium. These results suggest that conversion of mouse ES cells to EPL cells could constitute an important step in the directed differentiation strategy towards EC's.
@inproceedings{rao_differentiation_2002,
series = {{PROCEEDINGS} {OF} {ANNUAL} {INTERNATIONAL} {CONFERENCE} {OF} {THE} {IEEE} {ENGINEERING} {IN} {MEDICINE} {AND} {BIOLOGY} {SOCIETY}},
title = {Differentiation of mouse {EPL} (early primitive ectoderm like) cells into endothelial progenitors},
isbn = {0-7803-7612-9},
abstract = {Embryonic stem cell (ES) based therapies have gained significant interest due to their limitless self-renewal capabilities and potential formation of all tissue types. There is specific interest in our laboratory to produce endothelial cells (EC) from ES cells. Differentiation parameters that have been adapted have focused on the role of the vascular endothelial growth factor (VEGF). Recent studies and work done in our laboratory indicate that mouse ES cells, when grown on a collagen matrix, differentiate into EC progenitors as indicated by production of Flk1(+) (VEGF-receptor) cells. We have a novel technique for culturing stable early primitive ectoderm like (EPL) cells, which are obligatory intermediates in differentiation pathways, and derived from ES cells. Mouse EPL cells were grown on a collagen matrix and Flk1 expression was monitored over an 8-day time period. An early and increased production of Flk1(+) cells when mouse ES cells are converted to EPL cells, was observed. When EPL cells were also exposed to VEGF, endothelial tube structures started to form and networks of tubes were present after 4 days of VEGF in the culture medium. These results suggest that conversion of mouse ES cells to EPL cells could constitute an important step in the directed differentiation strategy towards EC's.},
booktitle = {{SECOND} {JOINT} {EMBS}-{BMES} {CONFERENCE} 2002, {VOLS} 1-3, {CONFERENCE} {PROCEEDINGS}: {BIOENGINEERING} - {INTEGRATIVE} {METHODOLOGIES}, {NEW} {TECHNOLOGIES}},
publisher = {Engn Med \& Biol Soc; Biomed Engn Soc; Natl Sci Fdn; Natl Inst Hlth; Natl Inst Biomed Imaging \& Bioengn; Whitaker Fdn},
author = {Rao, RR and Lyons, I and Stice, SL},
year = {2002},
note = {ISSN: 1094-687X},
pages = {682--683},
}
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Recent studies and work done in our laboratory indicate that mouse ES cells, when grown on a collagen matrix, differentiate into EC progenitors as indicated by production of Flk1(+) (VEGF-receptor) cells. We have a novel technique for culturing stable early primitive ectoderm like (EPL) cells, which are obligatory intermediates in differentiation pathways, and derived from ES cells. Mouse EPL cells were grown on a collagen matrix and Flk1 expression was monitored over an 8-day time period. An early and increased production of Flk1(+) cells when mouse ES cells are converted to EPL cells, was observed. When EPL cells were also exposed to VEGF, endothelial tube structures started to form and networks of tubes were present after 4 days of VEGF in the culture medium. These results suggest that conversion of mouse ES cells to EPL cells could constitute an important step in the directed differentiation strategy towards EC's.","booktitle":"SECOND JOINT EMBS-BMES CONFERENCE 2002, VOLS 1-3, CONFERENCE PROCEEDINGS: BIOENGINEERING - INTEGRATIVE METHODOLOGIES, NEW TECHNOLOGIES","publisher":"Engn Med & Biol Soc; Biomed Engn Soc; Natl Sci Fdn; Natl Inst Hlth; Natl Inst Biomed Imaging & Bioengn; Whitaker Fdn","author":[{"propositions":[],"lastnames":["Rao"],"firstnames":["RR"],"suffixes":[]},{"propositions":[],"lastnames":["Lyons"],"firstnames":["I"],"suffixes":[]},{"propositions":[],"lastnames":["Stice"],"firstnames":["SL"],"suffixes":[]}],"year":"2002","note":"ISSN: 1094-687X","pages":"682–683","bibtex":"@inproceedings{rao_differentiation_2002,\n\tseries = {{PROCEEDINGS} {OF} {ANNUAL} {INTERNATIONAL} {CONFERENCE} {OF} {THE} {IEEE} {ENGINEERING} {IN} {MEDICINE} {AND} {BIOLOGY} {SOCIETY}},\n\ttitle = {Differentiation of mouse {EPL} (early primitive ectoderm like) cells into endothelial progenitors},\n\tisbn = {0-7803-7612-9},\n\tabstract = {Embryonic stem cell (ES) based therapies have gained significant interest due to their limitless self-renewal capabilities and potential formation of all tissue types. 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