Bioinspired, Size-Tunable Self-Assembly of Polymer–Lipid Bilayer Nanodiscs. Ravula, T., Ramadugu, S., Di Mauro, G., & Ramamoorthy, A. Angewandte Chemie - International Edition, 2017. abstract bibtex © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Polymer-based nanodiscs are valuable tools in biomedical research that can offer a detergent-free solubilization of membrane proteins maintaining their native lipid environment. Herein, we introduce a novel ca. 1.6 kDa SMA-based polymer with styrene:maleic acid moieties that can form nanodiscs containing a planar lipid bilayer which are useful to reconstitute membrane proteins for structural and functional studies. The physicochemical properties and the mechanism of formation of polymer-based nanodiscs are characterized by light scattering, NMR, FT-IR, and TEM. A remarkable feature is that nanodiscs of different sizes, from nanometer to sub-micrometer diameter, can be produced by varying the lipid-to-polymer ratio. The small-size nanodiscs (up to ca. 30 nm diameter) can be used for solution NMR spectroscopy studies whereas the magnetic-alignment of macro-nanodiscs (diameter of > ca. 40 nm) can be exploited for solid-state NMR studies on membrane proteins.
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title = {Bioinspired, Size-Tunable Self-Assembly of Polymer–Lipid Bilayer Nanodiscs},
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year = {2017},
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abstract = {© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Polymer-based nanodiscs are valuable tools in biomedical research that can offer a detergent-free solubilization of membrane proteins maintaining their native lipid environment. Herein, we introduce a novel ca. 1.6 kDa SMA-based polymer with styrene:maleic acid moieties that can form nanodiscs containing a planar lipid bilayer which are useful to reconstitute membrane proteins for structural and functional studies. The physicochemical properties and the mechanism of formation of polymer-based nanodiscs are characterized by light scattering, NMR, FT-IR, and TEM. A remarkable feature is that nanodiscs of different sizes, from nanometer to sub-micrometer diameter, can be produced by varying the lipid-to-polymer ratio. The small-size nanodiscs (up to ca. 30 nm diameter) can be used for solution NMR spectroscopy studies whereas the magnetic-alignment of macro-nanodiscs (diameter of > ca. 40 nm) can be exploited for solid-state NMR studies on membrane proteins.},
bibtype = {article},
author = {Ravula, T. and Ramadugu, S.K. and Di Mauro, G. and Ramamoorthy, A.},
journal = {Angewandte Chemie - International Edition},
number = {38}
}
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